2014
DOI: 10.3389/fmicb.2014.00522
|View full text |Cite
|
Sign up to set email alerts
|

Assessing impacts of unconventional natural gas extraction on microbial communities in headwater stream ecosystems in Northwestern Pennsylvania

Abstract: Hydraulic fracturing and horizontal drilling have increased dramatically in Pennsylvania Marcellus shale formations, however the potential for major environmental impacts are still incompletely understood. High-throughput sequencing of the 16S rRNA gene was performed to characterize the microbial community structure of water, sediment, bryophyte, and biofilm samples from 26 headwater stream sites in northwestern Pennsylvania with different histories of fracking activity within Marcellus shale formations. Furth… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

2
42
1

Year Published

2015
2015
2022
2022

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 42 publications
(45 citation statements)
references
References 75 publications
(109 reference statements)
2
42
1
Order By: Relevance
“…Interestingly, Trexler et al . () also observed that WPS‐2 seems to co‐occur with methanotrophs in aquatic mosses and speculated that WPS‐2 may be using derivatives of methanotrophy such as carbon dioxide, formaldehyde, or formate. However, we found only two instances of the methane oxidation marker gene ( pmoA ) in our shotgun metagenomes (out of more than 926 million reads), therefore it seems unlikely that the highly abundant members of the WPS‐2 phylum found in our samples associate with methanotrophs.…”
Section: Resultsmentioning
confidence: 92%
See 2 more Smart Citations
“…Interestingly, Trexler et al . () also observed that WPS‐2 seems to co‐occur with methanotrophs in aquatic mosses and speculated that WPS‐2 may be using derivatives of methanotrophy such as carbon dioxide, formaldehyde, or formate. However, we found only two instances of the methane oxidation marker gene ( pmoA ) in our shotgun metagenomes (out of more than 926 million reads), therefore it seems unlikely that the highly abundant members of the WPS‐2 phylum found in our samples associate with methanotrophs.…”
Section: Resultsmentioning
confidence: 92%
“…Given how little we know about this phylum, we can only speculate about the ecology of this group from a few previous studies in which members of this phylum have been detected. Representatives from WPS‐2 have previously been found in acidic and cold environments, including alpine bog vegetation (Bragina et al ., ), mineral deposits from a low‐temperature acidic spring (Grasby et al ., ), and acidic natural gas extraction shale (Trexler et al ., ). Interestingly, Trexler et al .…”
Section: Resultsmentioning
confidence: 97%
See 1 more Smart Citation
“…All sequenced reads were quality filtered with Usearch7 [69] and analyzed with the Qiime 1.7.0 platform. [68,70] Relative abundances of Operational Taxonomic Units (OTU's) were computed at the genus-level.…”
Section: Field Methodsmentioning
confidence: 99%
“…[70] Analyzing a subset of our streams (6/24) for microbial communities in stream water revealed that specific microbial taxa within the Deltaproteobacteria shared a positive correlation to dissolved total mercury (FTHg) and to the number of well pads within a watershed (Table 3). Geobacter, which are known to methylate mercury in diverse environments, were positively correlated to dissolved THg.…”
Section: Mediamentioning
confidence: 99%