Assessing locomotor activity in larval zebrafish: Influence of extrinsic and intrinsic variables

Padilla, Stephanie; Hunter, Deborah L.; Padnos, Beth; Frady, S.; MacPhail, Robert C.

doi:10.1016/j.ntt.2011.08.005

Cited by 188 publications

(139 citation statements)

References 28 publications

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“…Then, a return to the basal activity period corresponding to a recovery phase was observed (light on (2)). This pattern of response is consistent with previous results reported in the literature MacPhail et al 2009;Padilla et al 2011). Concerning Japanese medaka, the potential toxicity of PAH on larval behavior assays has been only recently analyzed and only for PAHs in mixture (Le Bihanic et al 2014b).…”

Section: Discussionsupporting

confidence: 90%

“…The assessment of the developmental neurotoxic effects of various compounds can be analyzed through their swimming capacity, stress-regulating systems, and motor responses (perception and reaction) to stimuli (Champagne et al 2010;Drapeau et al 2002;de Esch et al 2012;Linney et al 2004). Studies regarding behavioral effects in fish have been extensively reported using psychotropic substances (Ali et al 2012;Cowden et al 2012;Kokel et al 2010;Padilla et al 2011;Rihel et al 2010) but poorly investigated for other pollutants. In the present study, a significant decrease of PMR in zebrafish was observed for the highest concentration of Fluo in the absence of peat.…”

Section: Discussionmentioning

confidence: 99%

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Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

Perrichon

Bihanic

Bustamante

et al. 2014

Environ Sci Pollut Res

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Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals testing. Second, a comparative study of toxicity responses from this exposure protocol was then performed using another OECD species, the Japanese medaka. The potential toxicity of both PAHs was assessed through lethal (e.g., survival, hatching success) and sublethal endpoints (e.g., abnormalities, PMR, and EROD) measured at different developmental stages, adapted to the embryonic development time of both species. Regarding effects observed for both species, a suitable artificial sediment composition for PAH toxicity testing was set at 92.5 % dry weight (dw) silica of 0.2-0.5-mm grain size, 5 % dw kaolin clay without organic matter for zebrafish, and 2.5 % dw blond peat in more only for Japanese medaka. PAH bioavailability and toxicity were highly dependent on the fraction of organic matter in sediment and of the K ow coefficients of the tested compounds. The biological responses observed were also dependent of the species under consideration. Japanese medaka embryos appeared more robust than zebrafish embryos for understanding the toxicity of PAHs following a sediment contact test, due to the longer exposure duration and lower sensitivity of sediment physical properties.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

Perrichon

Bihanic

Bustamante

et al. 2014

Environ Sci Pollut Res

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“…Embryos and larvae were scored for malformation at 72 hpf by visual check under a dissection scope by rating scale of (Padilla et al, 2011). According to the scale, it is assessed each embryo/larva for various categories (e.g.…”

Section: Methodsmentioning

confidence: 99%

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Şi̇şman¹,

Atamanalp²,

Dane³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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In this study, toxicity of water associated fraction of 10W-40 motor oil was investigated during the early life stages of zebrafish (Danio rerio). In 1.5-hour post fertilization (hpf), the fertilized zebrafish eggs were exposed to the oil fraction (1.25, 2.5, 5.0,10.0, 20.0 and 40.0 v/v) and were followed through 24, 48, and 72 hpf. The morphological endpoints of development toxicity of the oil in zebrafish embryos were observed under a stereomicroscope. Higher concentrations caused acute lethal effects, and lower concentrations caused sublethal effects, such as incomplete gastrulation, tail curvature, weak pigmentation, head malformation, pericardial edema, vertebra column defect and scoliosis. Moreover, with the increase of the oil concentration, the malformation rate increased. The hatching rate and body length were also decreased. Finally, it was found that the water associated fraction of 10W-40 motor oil affected the normal embryonic development of zebrafish.

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“…Com o desenvolvimento de novas tecnologias capazes de realizar análises automatizadas, a determinação de medidas de comportamento de organismos aquáticos tornouse possível. Estudos tem mostrado a elevada sensibilidade do comportamento de embriões e larvas de zebrafish quando comparado aos testes de toxicidade tradicionais (MACPHAIL et al, 2009;PADILLA et al, 2011;ANDRADE et al, 2016). A locomoção de larvas de zebrafish expostas ao fungicida carbendazim, por exemplo, mostrou elevada sensibilidade ao detectar problemas em concentrações 8.750 vezes abaixo do que aquelas capazes de alterar o desenvolvimento embrionário (ANDRADE et al, 2016).…”

Section: Comportamentounclassified

“…Além disso, alterações na locomoção de zebrafish possuem elevada correlação com danos neurológicos mediados por enzimas colinérgicas (MACPHAIL et al, 2009;SELDERSLAGHS et al, 2010;PADILLA et al, 2011;CHEN et al, 2011) e custos metabólicos envolvidos em processos de detoxificação (ANDRADE et al, 2015).…”

Section: Comportamentounclassified

Avaliação ecogenotoxicológica de corante natural extraído de micro-organismo

Abe¹

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Assessing locomotor activity in larval zebrafish: Influence of extrinsic and intrinsic variables

Cited by 188 publications

References 28 publications

Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

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Avaliação ecogenotoxicológica de corante natural extraído de micro-organismo

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