Assessing nociceptive sensitivity in mouse models of inflammatory and neuropathic trigeminal pain

Krzyzanowska, Agnieszka; Pittolo, Silvia; Cabrerizo, Marina; Sánchez-López, Jorge; Krishnasamy, Senthil; Venero, César; Avendaño, Carlos

doi:10.1016/j.jneumeth.2011.07.006

Cited by 51 publications

(60 citation statements)

References 35 publications

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“…A similar report in mice also found a loosely tied infraorbital nerve (ie CCI-ION model) provided only insignificant hypersensitivity contralaterally. 32 These mice models are in contrast to the original and many subsequent reports of bilateral hypersensitivity with the CCI-ION model in rats, which causes more demyelination and nerve injury after the entire nerve is tied. 33 The anti-nociceptive effectiveness for the mechanical sensitivity relief is also consistent with that described in the literature.…”

Section: Discussionmentioning

confidence: 97%

Efficacy of Herpes Simplex Virus Vector Encoding the Human Preproenkephalin Gene for Treatment of Facial Pain in Mice

Ma¹,

Wang²,

Yoder³

et al. 2016

J Oral Facial Pain Headache

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Aims To determine whether herpes simplex virus–based vectors can efficiently transduce mouse trigeminal ganglion (TG) neurons and attenuate preexisting nerve injury–induced whisker pad mechanical hypersensitivity in a trigeminal inflammatory compression (TIC) neuropathic pain model. Methods Tissue transduction efficiencies of replication-conditional and replication-defective vectors to mouse whisker pads after topical administration and subcutaneous injection were assessed using quantitative real-time PCR (qPCR). Tissue tropism and transgene expression were assessed using qPCR and reverse-transcriptase qPCR following topical application of the vectors. Whisker pad mechanical sensitivities of TIC-injured mice were determined using graduated von Frey fibers before and after application of human preproenkephalin expressing replication-conditional vector (KHPE). Data were analyzed using one-way analysis of variance (ANOVA) and post hoc tests. Results Transduction of target TGs was 8- to 50-fold greater after topical application than subcutaneous injection and ≥ 100-fold greater for replication-conditional than replication-defective vectors. Mean KHPE loads remained constant in TGs (4.5–9.8 × 104 copies/TG) over 3 weeks but were below quantifiable levels (10 copies/tissue) within 2 weeks of application in other nontarget cephalic tissues examined. Transgene expression in TGs was maximal during 2 weeks after topical application (100–200 cDNA copies/mL) and was below quantifiable levels (1 cDNA copy/mL) in all nontarget tissues. Topical KHPE administration reduced TIC-related mechanical hypersensitivity on whisker pads 4-fold (P < .05) for at least 1 week. Conclusion Topically administered KHPE produced a significant antinociceptive effect in the TIC mouse model of chronic facial neuropathic pain. This is the first report in which a gene therapeutic approach reduced trigeminal pain–related behaviors in an established pain state in mice.

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Section: Discussionmentioning

confidence: 97%

Efficacy of Herpes Simplex Virus Vector Encoding the Human Preproenkephalin Gene for Treatment of Facial Pain in Mice

Ma¹,

Wang²,

Yoder³

et al. 2016

J Oral Facial Pain Headache

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“…g . withdrawal responses or grooming) [55][28,56,57], in which the unlearned behaviours are elicited by mechanical stimulations using von Frey filaments [28] or thermal stimulation [30]. Such non-operant assessments may be relatively easy to execute; however, they evaluate innate behaviours that do not reveal cerebral processing of nociception.…”

Section: Discussionmentioning

confidence: 99%

Orofacial Neuropathic Pain Leads to a Hyporesponsive Barrel Cortex with Enhanced Structural Synaptic Plasticity

et al. 2016

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Chronic pain is a long-lasting debilitating condition that is particularly difficult to treat due to the lack of identified underlying mechanisms. Although several key contributing processes have been described at the level of the spinal cord, very few studies have investigated the supraspinal mechanisms underlying chronic pain. Using a combination of approaches (cortical intrinsic imaging, immunohistochemical and behavioural analysis), our study aimed to decipher the nature of functional and structural changes in a mouse model of orofacial neuropathic pain, focusing on cortical areas involved in various pain components. Our results show that chronic neuropathic orofacial pain is associated with decreased haemodynamic responsiveness to whisker stimulation in the barrel field cortex. This reduced functional activation is likely due to the increased basal neuronal activity (measured indirectly using cFos and phospho-ERK immunoreactivity) observed in several cortical areas, including the contralateral barrel field, motor and cingulate cortices. In the same animals, immunohistochemical analysis of markers for active pre- or postsynaptic elements (Piccolo and phospho-Cofilin, respectively) revealed an increased immunofluorescence in deep cortical layers of the contralateral barrel field, motor and cingulate cortices. These results suggest that long-lasting orofacial neuropathic pain is associated with exacerbated neuronal activity and synaptic plasticity at the cortical level.

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“…Hyperalgesia assessment in rodent orofacial pain models has been performed using various techniques such as mechanical stimulation with Semmes-Weinstein monofilaments [27, 28], dowel gnawing [29], thermal assessment [30, 31], facial expression [32], air puff stimulation [28], bite force assessment [33], face grooming patterns after formalin injection [34, 35] and anesthetic-induced hindpaw motor behavior [36]. These techniques required anesthetizing the animal [36], confinement or restraint of the animal [28, 29, 33], shaving the face of the rodent [30, 31], had a short duration of hyperalgesia [34, 35] or required human intervention [27, 32].…”

Section: 0 Discussionmentioning

confidence: 99%

“…These techniques required anesthetizing the animal [36], confinement or restraint of the animal [28, 29, 33], shaving the face of the rodent [30, 31], had a short duration of hyperalgesia [34, 35] or required human intervention [27, 32]. Human intervention, particularly human males, has been shown to have the potential to introduce experimenter bias [37].…”

Section: 0 Discussionmentioning

confidence: 99%

Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment

Widmer

Morris-Wiman

2015

Physiology & Behavior

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Introduction Assessment of oral motor behavior in a mouse is challenging due to the lack of currently available techniques that are non-invasive and allow long-term assessment in a home cage environment. The purpose of this study was to evaluate incising behavior using mouse chow attached to a three-dimensional force transducer that was mounted on the existing home cage. In addition, a persistent hyperalgesia condition was introduced to evaluate the sensitivity of the technique to identify incising behavioral changes. Methods Incising activity of CD-1 male and female mice (n=48) was evaluated over a 24 hour recording session during four baseline and six longitudinal hyperalgesia assessment sessions using custom written software. A pre-clinical persistent pain model was used to induce hyperalgesia in the masseter muscle by repetitive acidic saline injections. Sex and age differences were evaluated for multiple incising variables during both light and dark cycles during baseline and hyperalgesia conditions. Results Significant sex differences were found for multiple incising variables but not for age. Discrete incising frequencies were identified in the range of 4.6-10.4 Hz and were reproducibly found in both female and male mice. A significant shift to lower incising frequencies was observed after repetitive acidic saline injections compared to neutral saline injections. This shift to lower frequencies of incising returned to baseline levels after approximately four weeks but was statistically longer in female compared to male mice. Significant differences were also found for chow intake (reduced) and weight change during the hyperalgesia condition. No significant differences were found for total number of incisions or number of incising episodes per day or incising force. Conclusions The findings from this study support the use of recording three dimensional incising forces as a sensitive measure of incising behavior. This novel technique allowed the identification of specific incising variables that were differentially affected in female and male mice during a persistent hyperalgesia. The data were collected in the home cage environment with minimal bias such as experimenter interaction. Similar to other dental pain studies, mice were able to maintain normal incising activity levels per day (total incisions, total number of incising episodes) even in the presence of hyperalgesia.

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Assessing nociceptive sensitivity in mouse models of inflammatory and neuropathic trigeminal pain

Cited by 51 publications

References 35 publications

Efficacy of Herpes Simplex Virus Vector Encoding the Human Preproenkephalin Gene for Treatment of Facial Pain in Mice

Efficacy of Herpes Simplex Virus Vector Encoding the Human Preproenkephalin Gene for Treatment of Facial Pain in Mice

Orofacial Neuropathic Pain Leads to a Hyporesponsive Barrel Cortex with Enhanced Structural Synaptic Plasticity

Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment

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