Assessing off-target cytotoxicity of the field lampricide 3-trifluoromethyl-4-nitrophenol using novel lake sturgeon cell lines

Vo, Nguyen T. K.; Moore, Levi C.; Spiteri, Katelin W.; Hanner, Robert; Wilkie, Michael P.; DeWitte‐Orr, Stephanie J.

doi:10.1016/j.ecoenv.2018.07.017

Cited by 9 publications

(2 citation statements)

References 53 publications

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“…This distinction is not always clear, as has been noted for fin cell lines as well (Mauger et al, ). In the fish skin invitrome, epithelioid cell lines have been described from lake sturgeon and mudloach (Vo et al, ; Xu, Sivaramasamy, Jin, Li, & Xiang, ) and fibroblastoid ones, from rainbow trout and Japanese flounder (Ossum, Hoffmann, Vijayan, Holt, & Bols, ; Wang, Zhang, Wang, Wang, & Wang, ). Other skin cell lines from goldfish (Lee, Caldwell, & Gibbons, ), Leopard wrasse (Ma, Sun, Zeng, & Lu, ), red‐spotted grouper (Lei et al, ) and Ussuri catfish (Ou, Lei, He, Zhou, & Zhang, ) have been described as showing both morphologies, with the proportions of cells in cultures having a particular shape changing in complex ways.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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A walleye dermal fibroblastoid cell line, WE‐skin11f, was established and characterized. WE‐skin11f was immunocytochemically positive for two known dermal fibroblast protein markers: vimentin and collagen I. At passage 26, WE‐skin11f cultures contained both diploid and aneuploid populations. Ascorbic acid was required to produce extracellular collagen I fibres. Both of the skin fibroblastoid cell lines, WE‐skin11f and rainbow trout‐derived RTHDF, were not as good as the walleye caudal fin fibroblastoid cell line, WE‐cfin11f, at forming abundant dense extracellular collagen matrices. The thermobiology of WE‐skin11f was similar to that of other walleye cell lines with 26°C showing best temperature for growth and 4°C showing no growth but 100% viability. The transcript levels of b2m and mhIa genes of the major histocompatibility class I receptor in WE‐skin11f were largely similar at all temperatures examined (4, 14, 20 and 26°C). Cortisol had a variety of effects on WE‐skin11f cells: growth inhibition, morphological change from fibroblastoid to epithelioid, and enhancement of barrier function. Treatment of WE‐skin11f cells with the physiologically relevant concentration of 100 ng/ml cortisol inhibited collagen I synthesis and matrix formation. Thus, WE‐skin11f cell line could be useful in fish dermatology, endocrinology, and immunology research.

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Section: Introductionmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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“…The two cell morphologies have been existed in previous established fish skin cell lines. For example, the skin cells derived from lake sturgeon (Vo et al, 2018), mud Loach (Xu et al, 2016) and Ussuri catfish (Ou et al, 2014) displayed epithelial‐like shape, while the skin cells from Japanese flounder (Wang et al, 2017), leopard wrasse (Ma et al, 2013) and rainbow trout (Kim et al, 2018) were fibroblast‐like. There was also fish skin cell line that had cell shape changes during subculture (Lei et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Development and characterization of a skin cell line from Chinese perch (Siniperca chuatsi) and its application in aquatic animal viruses

Meng

Wang

et al. 2022

Journal of Fish Diseases

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Chinese perch (Siniperca chuatsi), an important fish for the aquaculture industry of China, is often affected by viral diseases. A stable and sensitive cell line can play an important role in virus identification and isolation, functional gene identification, virus pathogenic mechanism and antiviral immunity study. In the present study, a new cell line (S. chuatsi skin cell, SCSC) derived from the skin of S. chuatsi was established. The SCSC mainly consisted of fibroblastic‐like cells, which grew well in M199 medium supplemented with 10% foetal bovine serum at 25°C. Chromosome analysis revealed that the SCSC (44%) has a diploid chromosome number of 2n = 48. The SCSC can be transfected and expressed exogenous gene efficiently. It also showed high sensitivity to several aquatic animal viruses from different families including Rhabdoviridae, Iridoviridae and Reoviridae. In addition, RT‐PCR showed that S. chuatsi rhabdovirus (SCRV) started genome replication as early as 3 h post infection in the cells, which also induced the up‐regulation of a variety of immune‐related genes including these related to interleukin family, pattern recognition receptors, JAK–STAT pathway and interferon regulatory factors. In summary, current study provided a new tool in research of fish viruses and its interaction with host.

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Gene delivery into Siberian sturgeon cell lines by commercial transfection reagents

Lee

Nam

et al. 2019

In Vitro Cell.Dev.Biol.-Animal

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Assessing off-target cytotoxicity of the field lampricide 3-trifluoromethyl-4-nitrophenol using novel lake sturgeon cell lines

Cited by 9 publications

References 53 publications

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Development and characterization of a skin cell line from Chinese perch (Siniperca chuatsi) and its application in aquatic animal viruses

Gene delivery into Siberian sturgeon cell lines by commercial transfection reagents

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