Abstract:This study assesses the potential for the lipid production by the oleaginous yeast Cystobasidium oligophagum JRC1 using dairy industry waste cheese whey as a substrate. Cheese whey was used either untreated (UCW) or deproteinized (DCW) at different concentrations (25-100%) to serve as the carbon and energy source. Both UCW and DCW supported high biomass and lipid productivities. The biomass productivity of 0.076 ± 0.0004 and 0.124 ± 0.0021 g/L h, lipid productivity of 0.0335 ± 0.0004 and 0.0272 ± 0.0008 g/L h,… Show more
“…Cystobasidium oligophagum has been characterized to e ciently convert WP into lipid. The biomass and the lipid content were 43.1% and 6.38 g/L, respectively (Vyas et al, 2019). In another study, the biomass and lipid production of Cryptococcus curvatus from WP achieved 10.77 g/L and 63.4%, respectively (Carota et al, 2017).…”
Section: Discussionmentioning
confidence: 92%
“…Generally, very few yeasts possess β-galactosidase and galactose metabolism pathway, thus resulting in the impossibility to utilize lactose by most yeasts (Domingues et al 2010). In previous studies, only two oleaginous yeasts have been reported able to accumulate lipid from WP with a content of more than 40% (Carota et al, 2017;Vyas et al, 2019). Cystobasidium oligophagum has been characterized to e ciently convert WP into lipid.…”
Section: Discussionmentioning
confidence: 99%
“…It should be noted that this strain can even convert xylose into lipid, which is quite challenging for many other yeasts (Cho et al, 2018). Besides, it can utilize the sucrose, commonly used as a conventional carbon source, and galactose-based sugars in an e cient manner, revealing the existence of a developed galactosidase system (Carota et al, 2017;Vyas et al, 2019).…”
Section: Analysis On the Utilization Of Different Sugarsmentioning
BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess
“…Cystobasidium oligophagum has been characterized to e ciently convert WP into lipid. The biomass and the lipid content were 43.1% and 6.38 g/L, respectively (Vyas et al, 2019). In another study, the biomass and lipid production of Cryptococcus curvatus from WP achieved 10.77 g/L and 63.4%, respectively (Carota et al, 2017).…”
Section: Discussionmentioning
confidence: 92%
“…Generally, very few yeasts possess β-galactosidase and galactose metabolism pathway, thus resulting in the impossibility to utilize lactose by most yeasts (Domingues et al 2010). In previous studies, only two oleaginous yeasts have been reported able to accumulate lipid from WP with a content of more than 40% (Carota et al, 2017;Vyas et al, 2019). Cystobasidium oligophagum has been characterized to e ciently convert WP into lipid.…”
Section: Discussionmentioning
confidence: 99%
“…It should be noted that this strain can even convert xylose into lipid, which is quite challenging for many other yeasts (Cho et al, 2018). Besides, it can utilize the sucrose, commonly used as a conventional carbon source, and galactose-based sugars in an e cient manner, revealing the existence of a developed galactosidase system (Carota et al, 2017;Vyas et al, 2019).…”
Section: Analysis On the Utilization Of Different Sugarsmentioning
BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess
“…It should be noted that this strain can even convert xylose into lipid, which is quite challenging for many other yeasts [ 8 ]. Besides, it can utilize the sucrose, commonly used as a conventional carbon source, and galactose-based sugars in an efficient manner, revealing the existence of a developed galactosidase system [ 6 , 30 ]. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Actually, A. namibiae was the first oleaginous microorganism reported to produce lipid using SM as the feedstock. In previous studies, only two oleaginous yeasts have been reported able to accumulate lipid from WP with a content of more than 40% [ 6 , 30 ]. Cystobasidium oligophagum has been characterized to efficiently convert WP into lipid.…”
Background
Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts.
Results
In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key α-galactosidases, and GalG was key β-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner.
Conclusions
This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess.
Graphic abstract
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
