Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems

Shin, Min Kyoung; Yoon, Seung Hyun; Kim, Myung Hwui; Lyoo, Young S.; Suh, Seung-Won; Yoo, Han Sang

doi:10.4142/jvs.2015.16.1.25

Cited by 11 publications

(11 citation statements)

References 19 publications

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“…Serological tests mainly include the serum neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (ELISA), agar diffusion test, and complement fixation test. Specifically, ELISA tests have been a powerful approach to detect the infectious diseases-associated pathogens in serological tests because its several advantages, including good sensitivity and specificity ( Sattler et al, 2014 ; Shin et al, 2015 ). Given that feces may carry some infectious pathogens, stool testing for donor pigs is crucial to reduce the infectious risk of porcine FMT directly.…”

Section: Donor Selectionmentioning

confidence: 99%

Standardized Preparation for Fecal Microbiota Transplantation in Pigs

Chen

Tang

et al. 2018

Front. Microbiol.

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The intestine of pigs harbors a mass of microorganisms which are essential for intestinal homeostasis and host health. Intestinal microbial disorders induce enteric inflammation and metabolic dysfunction, thereby causing adverse effects on the growth and health of pigs. In the human medicine, fecal microbiota transplantation (FMT), which engrafts the fecal microbiota from a healthy donor into a patient recipient, has shown efficacy in intestinal microbiota restoration. In addition, it has been used widely in therapy for human gastrointestinal diseases, including Clostridium difficile infection, inflammatory bowel diseases, and irritable bowel syndrome. Given that pigs share many similarities with humans, in terms of anatomy, nutritional physiology, and intestinal microbial compositions, FMT may also be used to restore the normal intestinal microbiota of pigs. However, feasible procedures for performing FMT in pigs remains unclear. Here, we summarize a standardized preparation for FMT in pigs by combining the standard methodology for human FMT with pig production. The key issues include the donor selection, fecal material preparation, fecal material transfer, stool bank establishment, and the safety for porcine FMT. Optimal donors should be selected to ensure the efficacy of porcine FMT and reduce the risks of transmitting infectious diseases to recipients during FMT. Preparing for fresh fecal material is highly recommended. Alternatively, frozen fecal suspension can also be prepared as an optimal choice because it is convenient and has similar efficacy. Oral administration of fecal suspension could be an optimal method for porcine fecal material transfer. Furthermore, the dilution ratio of fecal materials and the frequency of fecal material transfer could be adjusted according to practical situations in the pig industry. To meet the potential large-scale requirement in the pig industry, it is important to establish a stool bank to make porcine FMT readily available. Future studies should also focus on providing more robust safety data on FMT to improve the safety and tolerability of the recipient pigs. This standardized preparation for porcine FMT can facilitate the development of microbial targeted therapies and improve the intestinal health of pigs.

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Section: Donor Selectionmentioning

confidence: 99%

Standardized Preparation for Fecal Microbiota Transplantation in Pigs

Chen

Tang

et al. 2018

Front. Microbiol.

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“…Porcine circovirus type 2 (PCV2) is an essential pathogen of porcine circovirus-associated diseases (PCVADs) and caused great economic loss in pig breeding [123]. The main approach to control and prevents PCV2 infection is vaccination in pig industry [4]. At present, commercial inactivated vaccines based on PCV2 have been shown to be effective to decrease mortality and increase growth parameters in commercial pig herds.…”

Section: Introductionmentioning

confidence: 99%

Enhanced protective immune response to PCV2 adenovirus vaccine by fusion expression of Cap protein with InvC in pigs

et al. 2019

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The major immunogenic protein capsid (Cap) of porcine circovirus type 2 (PCV2) is critical to induce neutralizing antibodies and protective immune response against PCV2 infection. This study was conducted to investigate the immune response of recombinant adenovirus expressing PCV2b Cap and C-terminal domain of Yersinia pseudotuberculosis invasin (Cap-InvC) fusion protein in pigs. The recombinant adenovirus rAd-Cap-InvC, rAd-Cap and rAd were generated and used to immunize pigs. The phosphate-buffered saline was used as negative control. The specific antibodies levels in rAd-Cap-InvC and ZJ/C-strain vaccine groups were higher than that of rAd-Cap group ( p < 0.05), and the neutralization antibody titer in rAd-Cap-InvC group was significantly higher than those of other groups during 21–42 days post-immunization (DPI). Moreover, lymphocyte proliferative level, interferon-γ and interleukin-13 levels in rAd-Cap-InvC group were increased compared to rAd-Cap group ( p < 0.05). After virulent challenge, viruses were not detected from the blood samples in rAd-Cap-InvC and ZJ/C-strain vaccine groups after 49 DPI. And the respiratory symptom, rectal temperature, lung lesion and lymph node lesion were minimal and similar in the ZJ/C-strain and rAd-Cap-InVC groups. In conclusion, our results demonstrated that rAd-Cap-InvC was more efficiently to stimulate the production of antibody and protect pigs from PCV2 infection. We inferred that InvC is a good candidate gene for further development and application of PCV2 genetic engineering vaccine.

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“…First evidence of their existence was their detection in a porcine kidney cell line (PK15) leading to the name porcine circovirus type 1 (Tischer et al, 1974). More than two decades later a second porcine circovirus, PCV2, was detected and made responsible for a couple of syndromes including post weaning multisystemic wasting syndrome, porcine dermatitis and nephropathy syndrome and PCV2-associated reproductive failure which were summarized later under the so-called porcine circovirus associated disease (PCVAD) (Limsaranrom et al, 2015;Meng, 2012;Pearodwong et al, 2015;Shin et al, 2015). Currently four genotypes namely PCV2a, PCV2b, PCV2c and PCV2d-mPCV2b are recognized (Franzo et al, 2015).…”

mentioning

confidence: 99%

Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs

Herbst

Willems

2017

Research in Veterinary Science

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During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768bp. The nucleotide sequence was highly similar (98% nucleotide identity) to the PCV2a reference sequence.

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Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems

Cited by 11 publications

References 19 publications

Standardized Preparation for Fecal Microbiota Transplantation in Pigs

Standardized Preparation for Fecal Microbiota Transplantation in Pigs

Enhanced protective immune response to PCV2 adenovirus vaccine by fusion expression of Cap protein with InvC in pigs

Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs

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