2022
DOI: 10.1016/j.omtm.2022.11.003
|View full text |Cite
|
Sign up to set email alerts
|

Assessing production variability in empty and filled adeno-associated viruses by single molecule mass analyses

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
37
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 35 publications
(39 citation statements)
references
References 43 publications
2
37
0
Order By: Relevance
“…The longer ssDNA construct most likely represents an elongation of the ssDNA genome with part of the plasmid backbone to the maximum loading capacity of a rAAV particle. Similar findings were also observed by another study using charge detection mass spectrometry as well as mass photometry . This effect might be due to the fact that shorter ssDNA has not had the optimal loading length for rAAV particles and therefore can result in unwanted elongation with the production plasmid backbone.…”
Section: Resultssupporting
confidence: 85%
“…The longer ssDNA construct most likely represents an elongation of the ssDNA genome with part of the plasmid backbone to the maximum loading capacity of a rAAV particle. Similar findings were also observed by another study using charge detection mass spectrometry as well as mass photometry . This effect might be due to the fact that shorter ssDNA has not had the optimal loading length for rAAV particles and therefore can result in unwanted elongation with the production plasmid backbone.…”
Section: Resultssupporting
confidence: 85%
“…Overall, Orbitrap-based CDMS is particularly well-adapted for a thorough characterization of AAVs, which fall in a size regime where only a few analytical techniques are available. As such, CDMS is being rapidly adopted by several academic and industrial laboratories, ,, with the technique even considered to be one of the gold standard methods to quantify capsid content …”
Section: Mass Spectrometry Of Biomolecular Assembliesmentioning
confidence: 99%
“…As Orbitrap signals in a native MS experiment are commonly averaged prior to analysis, the resulting peaks in the mass spectrum will broaden, leading to the loss of mass resolving power and obfuscating individual charge peaks. With applications of native MS moving away from the historical norms of purified recombinant proteins and toward progressively more complicated mixtures (e.g., plasma glycoproteins, 25 membrane-embedded proteins, 26 proteins with multiple ligands, 27 vaccines and gene-delivery vectors 28 ), there is a growing need to tackle the charge inferencing problem. One way to avoid problematic signal convolution may come from measuring each ion individually.…”
Section: Assembliesmentioning
confidence: 99%
“…CDMS can be used to determine the masses of large, heterogeneous samples that cannot be analyzed via traditional MS techniques. CDMS simultaneously measures the charge and the mass‐to‐charge ratio of single ions, therefore alleviating the frequent problem of overlapping charge states in heterogeneous samples that can prevent accurate deconvolution of m/z spectra (Jarrold, 2022), especially for macromolecules/assemblies such as the SARS‐Cov‐2 spike glycoprotein complex (Miller, Barnes, et al, 2021), intact viral particles (e.g., adeno‐associated virus as gene‐therapy vectors) (Ebberink et al, 2022; Miller, Bond, et al, 2021), exosomes (Brown et al, 2020), and lipoproteins (Lutomski et al, 2018). However, intact mass measurement alone provides limited information.…”
Section: Interrogating the Heterogeneity Of Ptms On Endogenous Proteinsmentioning
confidence: 99%