Assessing the activity of antibodies conjugated to upconversion nanoparticles for immunolabeling

Cao, Yueying; Wu, Jiehua; Zheng, Xianlin; Lü, Yanling; Piper, James A.; Lu, Yiqing; Packer, Nicolle H.

doi:10.1016/j.aca.2022.339863

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…Furthermore, proper surface modification of nanoparticles with targeting ligands, 92 , 93 antibodies, 94 or oligonucleotides 95 can potentially improve tissue accumulation. It is worth noting that membranes derived from target cells can be coated on the surface of biomaterials, providing further improvements in delivery efficiency.…”

Section: Discussionmentioning

confidence: 99%

Nano-Proteolysis Targeting Chimeras (Nano-PROTACs) in Cancer Therapy

Song,

Dong,

et al. 2024

IJN

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Section: Discussionmentioning

confidence: 99%

Nano-Proteolysis Targeting Chimeras (Nano-PROTACs) in Cancer Therapy

Song,

Dong,

et al. 2024

IJN

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“…Using this conjugation method (Figure 1B), the active sites of receptors are exposed to the target antigen to maximize binding (Pollok et al., 2019). Due to their affinity with amines, common direct attachment of antibody to signal tags in LFAs use the primary amine functional group on the antibody with 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide hydrochloride (EDC) and/or N ‐hydroxysulfosuccinimide (Sulfo‐NHS) as coupling reagents (Cao et al., 2022). Hence, NHS is a major amine‐coupling reagent that is used in the labeling of antibodies (Hansen et al., 2022).…”

Section: Competitive Immobilizationmentioning

confidence: 99%

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

Li,

Jallow,

Nidiaye

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxins are dangerous human and animal health‐threatening secondary fungal metabolites that can be found in various food and agricultural products. Several countries have established regulations to restrict their presence in food and agricultural products destined for human and animal consumption. Consequently, the need to develop highly sensitive and smart detection systems was recognized worldwide. Lateral flow assay possesses the advantages of easy operation, rapidity, stability, accuracy, and specificity, and it plays an important role in the detection of mycotoxins. Nevertheless, strategies to comprehensively improve the sensitivity of lateral flow assay to mycotoxins in food have rarely been highlighted and discussed. In this article, a comprehensive overview was presented on the application of lateral flow assay in mycotoxin detection in food samples by highlighting the principle of lateral flow assay, presenting a detailed discussion on various analytical performance‐improvement strategies, such as the development of high‐affinity recognition reagents, immunogen immobilization methods, and signal amplification. Additionally, a detailed discussion on the various signal analyzers and interpretation approaches was provided. Finally, current hurdles and future perspectives on the application of lateral flow assay in the detection of mycotoxins were discussed.

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“…Lanthanide-doped up conversion nanoparticles (UCNPs) have emerged as a new group of luminescence bio labels, well known due to their photostability. In this work, the authors determine the most efficient number of antibodies to polysaccharide coated nanoparticles defining an optimum amount of 12 to 16 antibodies per nanoparticle to preserve an adequate binding capacity and good performance over different applications such as ELISA, flow cytometry and cellular imaging (42).…”

Section: Antibodiesmentioning

confidence: 99%