Assessing the biological reactivity of organic compounds on volcanic ash: implications for human health hazard

Tomašek, Ines; Damby, David E.; Andronico, Daniele; Baxter, Peter; Boonen, Imke; Claeys, Philippe; Denison, Michael S.; Horwell, Claire J.; Kervyn, Matthieu; Kueppers, Ulrich; Romanías, Manolis N.; Elskens, Marc

doi:10.1007/s00445-021-01453-4

Cited by 20 publications

(10 citation statements)

References 57 publications

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“…The origin of the carbonaceous matter is unknown, but can be due to burning vegetation or entrainment of organic material during the eruption. Observation of carbonaceous matter in this respirable volcanic ash further substantiates that people are rarely exposed to volcanic PM alone (Tomašek, Damby, Andronico, et al., 2021 ).…”

Section: Discussionsupporting

confidence: 68%

Spatial Distribution and Physicochemical Properties of Respirable Volcanic Ash From the 16–17 August 2006 Tungurahua Eruption (Ecuador), and Alveolar Epithelium Response In‐Vitro

et al. 2022

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Tungurahua volcano (Ecuador) intermittently emitted ash between 1999 and 2016, enduringly affecting the surrounding rural area and its population, but its health impact remains poorly documented. We aim to assess the respiratory health hazard posed by the 16-17 August 2006 most intense eruptive phase of Tungurahua. We mapped the spatial distribution of the health-relevant ash size fractions produced by the eruption in the area impacted by ash fallout. We quantified the mineralogy, composition, surface texture, and morphology of a respirable ash sample isolated by aerodynamic separation. We then assessed the cytotoxicity and pro-inflammatory potential of this respirable ash towards lung tissues in-vitro using A549 alveolar epithelial cells, by electron microscopy and biochemical assays. The eruption produced a high amount of inhalable and respirable ash (12.0-0.04 kg/m2 of sub-10 µm and 5.3-0.02 kg/m2 of sub-4 µm ash deposited). Their abundance and proportion vary greatly across the deposit within the first 20 km from the volcano. The respirable ash is characteristic of an andesitic magma and no crystalline silica is detected. Morphological features and surface textures are complex and highly variable, with few fibres observed. In-vitro experiments show that respirable volcanic ash is internalized by A549 cells and processed in the endosomal pathway, causing little cell damage, but resulting in changes in cell Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site.morphology and membrane texture. The ash triggers a weak pro-inflammatory response. These data provide the first understanding of the respirable ash hazard near Tungurahua and the extent to which it varies spatially in a fallout deposit. Key Points► The 16-17 August 2006 Tungurahua eruption produced a high amount of inhalable (12.0-0.04 kg/m2 of sub-10 µm deposited on the ground) and respirable ash (5.3-0.02 kg/m2 of sub-4 µm ash deposited on the ground). The abundance and proportion of inhalable and respirable ash vary greatly across the deposit within the first 20 km from the volcano ► The respirable ash is characteristic of an andesitic magma and no crystalline silica is detected. Morphological features and surface textures are complex and highly variable, with few fibres observed ► In-vitro experiments show that respirable volcanic ash is internalized by A549 cells and processed in the endosomal pathway, causing little cell damage, but resulting in changes in cell morphology and membrane texture. The ash triggers a weak pro-inflammatory response Plain Language SummaryMuch is left to understand on the health hazard related to inhaling volcanic ash. This is due to the high variability in ash properties from one volcanic eruption to another. In our new study, we focus on a key volcanic environment, the region of Tungurahua volcano in the Eastern Andean Cordillera of Ecuador, where populations were affected by an ex...

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Section: Discussionsupporting

confidence: 68%

Spatial Distribution and Physicochemical Properties of Respirable Volcanic Ash From the 16–17 August 2006 Tungurahua Eruption (Ecuador), and Alveolar Epithelium Response In‐Vitro

et al. 2022

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“…Although AOPs are not chemical- or stressor-specific, TCDD has been widely used as a compound of reference in toxicology and risk assessment 33–36 . Therefore, knowing other MIE/AOP that it triggers is of great significance, especially if they can be applicable to humans.…”

Section: Resultsmentioning

confidence: 99%

Proteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways

Lizano-Fallas

Amor

Cristóbal

2022

Preprint

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Understanding the biological impact of chemicals is hindered by the high number and diversity of compounds in the market. To simplify the chemical risk assessment, the adverse outcome pathway (AOP) method has arisen as a framework to predict the impact of chemical exposure on human and environmental health. The development of this predictive tool requires knowledge of the molecular interaction between chemicals and protein targets. Those molecular initiating events connect alterations of cellular function with physiological impairment. This strategy aims to focus on the complex biological interaction to predict the impact on health. The high-throughput identification of all chemical targets can be obtained by a proteomics-based thermal shift assay, however, selecting the priority target candidate is a biased process strongly dependent on expert knowledge and literature. Here, we unravel new molecular initiating event from a tested chemical combining the target deconvolution by the proteome integral solubility alteration (PISA) assay, and the target selection by an analytical hierarchy process (AHP) approach. In the proof-of-concept study, we identified by PISA assay 8 protein targets for 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) from the soluble proteome from hepatic cells containing 2824 proteins. The definition of the AHP approach facilitates the selection of heat shock protein beta-1 (Hspb1) as the most suitable protein for developing AOPs. Our results demonstrated that the process of target identification is independent from a chemical characterization, and that the process of data curation and target selection is less sensitive to lack of toxicological information. We anticipate that this innovative integration of methods could decipher the chemical-protein interactions from new chemicals including the new alternative chemicals designed for chemical replacement and that would discover new molecular initiating events to support more sustainable methodologies to gain time and resources in chemicals assessment.SYNOPSISOur combined methodologies can determine the most suitable target to develop adverse outcome pathways from the proteome-wide protein target identification.

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“…Observation of carbonaceous matter in this respirable volcanic ash further substantiates that people are rarely exposed to volcanic PM alone. 67…”

Section: Discussionmentioning

confidence: 99%

Spatial distribution and physicochemical properties of respirable volcanic ash from the 16-17 August 2006 Tungurahua eruption (Ecuador), and alveolar epithelium responsein-vitro

Eychenne

Gurioli

Damby

et al. 2022

Preprint

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Background: Tungurahua volcano (Ecuador) intermittently emitted ash between 1999 and 2016, enduringly affecting the surrounding rural area and its population, but its health impact remains poorly documented. Objectives: We aim at assessing the respiratory health hazard posed by the 16-17 August 2006 most intense eruptive phase of Tungurahua. Methods: Based on detailed field surveys and grain size analyses, we mapped the spatial distribution of the health-relevant ash size fractions produced by the eruption in the area impacted by ash fallout. We used Scanning Electron Microscopy and Raman Spectroscopy to quantify the mineralogy, composition, surface texture and morphology of a respirable ash sample isolated by aerodynamic separation. The cytotoxicity and pro-inflammatory potential of this respirable ash towards lung tissues was assessed in-vitro using A549 alveolar epithelial cells, by Electron Microscopy and biochemical assays (LDH assay, RT-qPCR, multiplex immunoassays). Results: The eruption produced a high amount of inhalable and respirable ash (12.0-0.04 kg/m2 of sub-10 um and 5.3-0.02 kg/m2 of sub-4 um ash deposited). Their abundance and proportion vary greatly across the deposit within the first 20 km from the volcano. The respirable ash is characteristic of an andesitic magma and no crystalline silica is detected. Morphological features and surface textures are complex and highly variable, with few fibres observed. In-vitro experiments show that respirable volcanic ash are internalized by A549 cells and processed in the endosomal pathway, causing little cell damage, but some changes in cell morphology and membrane texture. The ash trigger a weak pro-inflammatory response. Discussion: These data provide the first understanding of the respirable ash hazard near Tungurahua, and the extent to which it varies spatially in a fallout deposit. Given the long exposure duration of the surrounding population, the chronic effects of this inhalable, weakly bio-reactive ash on health could be further investigated.

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Assessing the biological reactivity of organic compounds on volcanic ash: implications for human health hazard

Cited by 20 publications

References 57 publications

Spatial Distribution and Physicochemical Properties of Respirable Volcanic Ash From the 16–17 August 2006 Tungurahua Eruption (Ecuador), and Alveolar Epithelium Response In‐Vitro

Spatial Distribution and Physicochemical Properties of Respirable Volcanic Ash From the 16–17 August 2006 Tungurahua Eruption (Ecuador), and Alveolar Epithelium Response In‐Vitro

Proteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways

Spatial distribution and physicochemical properties of respirable volcanic ash from the 16-17 August 2006 Tungurahua eruption (Ecuador), and alveolar epithelium responsein-vitro

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