Assessing the correlation between the degree of disc degeneration on the Pfirrmann scale and the metabolites identified in HR-MAS NMR spectroscopy

Radek, Maciej; Pacholczyk‐Sienicka, Barbara; Jankowski, Stefan; Albrecht, Łukasz; Grodzka, Magdalena; Depta, Adam; Radek, A

doi:10.1016/j.mri.2015.12.005

Cited by 22 publications

(19 citation statements)

References 15 publications

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“…MRI, with its high sensitivity to the tissue water content, has become an accurate tool for assessing IDD (8). Although the five-level Pfirrmann grading system is commonly used in clinical practice, there is a lack of information about biochemical changes that occur in the disc (9). At present, numerous quantitative MRI diagnostic methods have already been used to quantitatively explore the process of IDD, which include in vivo 23 Na MRI, quantitative high-resolution magic angle spinning nuclear MR spectroscopy, proton T2 imaging and T1rho imaging (10).…”

Section: Introductionmentioning

confidence: 99%

Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy

et al. 2020

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Lower back pain (LBP) is one of the most common musculoskeletal complaints worldwide. Intervertebral disc degeneration (IDD) is considered to be a significant contributor to LBP; however, the mechanisms underlying IDD remain to be fully elucidated. One of the major features of IDD is the decreased content of type II collagen and proteoglycans in the nucleus pulposus (NP). The present study aimed to investigate the biochemical mechanisms of IDD at the microscopic level using Raman spectroscopy. Raman spectroscopy, based on inelastic scattering of light, is an emerging optical technique that may measure the chemical composition of complex biological samples, including biofluids, cells and tissues. In the present study, 30 NP tissue samples from 30 patients who were diagnosed with lumbar disc herniation and received spinal fusion surgery to relieve LBP were obtained and analyzed. Routine pre-operative 3.0T, T2-weighed MRI was used to classify the cases according to Pfirrmann grades and the T2 signal intensity value of the NP was measured. Subsequently, all NP samples were scanned and analyzed using a Laser MicroRaman Spectrometer at room temperature. The Raman spectral results demonstrated that the relative content of proteoglycans, expressed as the relative intensity ratio of two peaks (I 1064 /I 1004), was significantly inversely correlated with the Pfirrmann grade (ρ=-0.6462; P<0.0001), whereas the content of collagen (amide I) was significantly positively correlated with the Pfirrmann grade (ρ=0.5141; P<0.01). In conclusion, the higher relative intensity of the ratio of two peaks (I 1670 /I 1640 ; Amide I) represented a higher fractional content of disordered collagen, which suggested that the defective collagen structure may lead to NP abnormalities.

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Section: Introductionmentioning

confidence: 99%

Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy

et al. 2020

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“…A previous study demonstrated that the loss of signal intensity in the NP area is significantly associated with the morphological features and biochemical contents of degenerative human IVDs[46]. Furthermore, the expression of catabolic factors, such as proinflammatory cytokines and matrix-degrading enzymes, was upregulated in the degenerated human IVD evaluated by MRI[47–51]. We, therefore, defined an MRI classification of more than grade IV as ‘advanced stage of degeneration.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of DNA methylation profile identifies differentially methylated loci associated with human intervertebral disc degeneration

et al. 2019

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BackgroundEnvironmental and endogenous factors under genetic predisposition are considered to initiate the human intervertebral disc (IVD) degeneration. DNA methylation is an essential mechanism to ensure cell-specific gene expression for normal development and tissue stability. Aberrant epigenetic alterations play a pivotal role in several diseases, including osteoarthritis. However, epigenetic alternations, including DNA methylation, in IVD degeneration have not been evaluated. The purpose of this study was to comprehensively compare the genome-wide DNA methylation profiles of human IVD tissues, specifically nucleus pulpous (NP) tissues, with early and advanced stages of disc degeneration.MethodsHuman NP tissues were used in this study. The samples were divided into two groups: early stage degeneration (n = 8, Pfirrmann’s MRI grade: I-III) and advanced stage degeneration (n = 8, grade: IV). Genomic DNA was processed for genome-wide DNA methylation profiling using the Infinium MethylationEPIC BeadChip array. Extraction of raw methylation data, clustering and scatter plot of each group values of each sample were performed using a methylation module in GenomeStudio software. The identification of differentially methylated loci (DMLs) and the Gene Ontology (GO) analysis were performed using R software with the ChAMP package.ResultsUnsupervised hierarchical clustering revealed that early and advanced stage degenerated IVD samples segregated into two main clusters by their DNA methylome. A total of 220 DMLs were identified between early and advanced disc degeneration stages. Among these, four loci were hypomethylated and 216 loci were hypermethylated in the advanced disc degeneration stage. The GO enrichment analysis of genes containing DMLs identified two significant GO terms for biological processes, hemophilic cell adhesion and cell-cell adhesion.ConclusionsWe conducted a genome-wide DNA methylation profile comparative study and observed significant differences in DNA methylation profiles between early and advanced stages of human IVD degeneration. These results implicate DNA methylation in the process of human IVD degeneration.

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“…Isoleucine, valine and leucine are branched chain amino acids. Branched chain amino acids are one of the disc components [ 21 ]. Leucine intake was associated with long-term lean body mass retention [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…One of the useful biomarkers related to cartilage turnover, that might predict progressive knee osteoarthritis, is serum hyaluronic acid which leaks into the blood from degenerated cartilage [ 20 ]. Amino acids are chemical constituents of the intervertebral disc [ 21 ]; however, there are only a few reports about useful biomarkers for prediction of cervical intervertebral degeneration.…”

Section: Introductionmentioning

confidence: 99%

A study of the factors associated with cervical spinal disc degeneration, with a focus on bone metabolism and amino acids, in the Japanese population: a cross sectional study

Wada

Tanaka

Kumagai

et al. 2018

BMC Musculoskelet Disord

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BackgroundThe physical and biochemical factors responsible for cervical disc degeneration, and resulting in various spinal disorders, remain unclear. This study aimed to evaluate the correlation between cervical spinal canal stenosis and degeneration of intervertebral discs, and to analyze the factors related to disc degeneration in the Japanese population.MethodsThree hundred and forty-four Japanese general residents underwent investigations, including magnetic resonance imaging of the cervical spine, in our health check project. We measured anteroposterior diameters at the levels of the cervical spinal disc in mid sagittal plane magnetic resonance imaging and evaluated disc degeneration. Spearman correlation coefficient was used to evaluate whether the diameters were correlated with disc degenerative scores. Stepwise multiple linear regression analysis was conducted with the score of disc degeneration as the dependent variable; and age, physical measurement values, bone mineral density of the forearm, and the value of serum bone metabolic markers and amino acids as the independent variables for each sex.ResultsAs the age increased, the anteroposterior diameters decreased in both sexes. The minimum anteroposterior diameters were correlated with the disc degenerative scores (Spearman r = − 0.59, p < 0.001 in men, Spearman r = − 0.53, p < 0.001 in women). In multiple linear regression analysis, age, cross-linked N-telopeptide of type 1 collagen and isoleucine were significantly correlated with the cervical disc degenerative score in men (R2 = 0.47), and age and lysine were significantly correlated with the degenerative score in women (R2 = 0.50).ConclusionThe factors responsible for cervical disc degeneration differed between men and women. Whether modifying these significant factors is possible, or whether this intervention would contribute to prevention of disc degeneration requires future studies.

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Assessing the correlation between the degree of disc degeneration on the Pfirrmann scale and the metabolites identified in HR-MAS NMR spectroscopy

Cited by 22 publications

References 15 publications

Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy

Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy

Genome-wide analysis of DNA methylation profile identifies differentially methylated loci associated with human intervertebral disc degeneration

A study of the factors associated with cervical spinal disc degeneration, with a focus on bone metabolism and amino acids, in the Japanese population: a cross sectional study

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