Assessing the effects of gut bacteria manipulation on the development of the oriental fruit fly, Bactrocera dorsalis (Diptera; Tephritidae)

Khaeso, Kanjana; Andongma, Awawing A.; Akami, Mazarin; Souliyanonh, Biangkham; Zhu, Jian; Krutmuang, Patcharin; Niu, Changying

doi:10.1007/s13199-017-0493-4

Cited by 45 publications

(69 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These experiments contained two groups, namely, control (CK) and 1 μg/g azadirachtin treatment (Tr). The effects of azadirachtin on larval survival, developmental duration, and pupal weight were determined in accordance with a previous report and were briefly summarized below 37 . The stock solution of 10,000 μg/mL azadirachtin was prepared with acetone and stored at 4 °C.…”

Section: Methodsmentioning

confidence: 97%

Integrated LC–MS and GC–MS-based untargeted metabolomics studies of the effect of azadirachtin on Bactrocera dorsalis larvae

You

Qin

Zhang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Azadirachtin exhibits excellent bioactivities against several hundred arthropods. However, current knowlege of its biochemical effect on B. dorsalis larvae is not deep enough. In this study, integrated LC-MS and GC-MS-based untargeted metabolomics were used to analyze the changes of endogenous metabolites and the biochemical effects of azadirachtin on B. dorsalis larvae. Azadirachtin has excellent bioactivities against B. dorsalis larvae in this study, leading to a longer developmental duration, lower survival rate, and low pupa weight. The effect of azadirachtin was investigated on a total of 22 and 13 differentially abundant metabolites in the LC-MS and GC-MS-based metabolomics results, are selected respectively. Pathway analysis indicated that 14 differentially enriched metabolic pathways, including seven influential pathways, are worthy of attention. Further integrated key metabolic pathway analysis showed that histidine metabolism, d-glutamine and d-glutamate metabolism, biotin metabolism, ascorbate and aldarate metabolism, pentose and glucuronate interconversions, and alanine, aspartate and glutamate metabolism in B. dorsalis larvae are significantly relevant pathways affected by azadirachtin. Although extrapolating the bioactivity results in this study to the practical project of B. dorsalis pest management in the field has limitations, it was found that azadirachtin has a significant effect on the primary metabolism of B. dorsalis larvae.Bactrocera dorsalis is a destructive polyphagous and invasive insect pest of tropical and subtropical fruits and vegetables; this oriental fruit fly has been found to attack many types of commercial fruits and a wide variety of agricultural products 1 . Azadirachtin exhibits excellent bioactivities against agricultural, forestry, medical, and veterinary arthropods 2-4 . However, studies on the effects of azadirachtin on B. dorsalis are scarce. Azadirachtin is the main active ingredient in neem. It was reported that neem derivatives are ineffective when used as toxic bait for tephritid fruit flies 5 . Several studies reported that neem seed kernel extracts and azadirachtin deters oviposition of B. dorsalis adults 6,7 . Neem leaf dust significantly reduced the longevity and fertility of B. dorsalis adults by blocking ovarian development 8 . Neem extract could effectively reduce the fecundity, fertility, and post-embryonic development of freshly emerged B. dorsalis flies 9 . However, we found no previous studies in the literature on the activity of azadirachtin against the larvae of B. dorsalis.The biological effects of azadirachtin include impacts on egg-laying behavior, feeding behavior, growth and metamorphosis, reproduction, activity, and histopathology 10 . The mode of action of azadirachtin against lepidopteran insects can be explained, in part, by effects on digestive enzymes, NADPH cytochrome reductase, and cholinesterase 11 . The physiological effects of azadirachtin include direct inhibition of cell division and protein synthesis 12 . The indirect effects of blocki...

show abstract

Section: Methodsmentioning

confidence: 97%

Integrated LC–MS and GC–MS-based untargeted metabolomics studies of the effect of azadirachtin on Bactrocera dorsalis larvae

You

Qin

Zhang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Other bacterial OTUs like Listeria (Listeriaceae), Pseudomonas aeruginosa (Schr€ oter) Migula (Pseudomonadaceae), Enterococcus sp., (Enterococcaceae), and Lactobacillus sp. (Streptococcaceae) were also identified from B. dorsalis but they were less abundant (Gujjar et al, 2017;Khaeso et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Stathopoulou

Asimakis

Khan

et al. 2019

Entomologia Exp Applicata

View full text Add to dashboard Cite

The role of symbiotic microbes in insects, especially the beneficial character of this interaction for insects, has received much attention in recent years as it has been related to important aspects of the host insects' biology such as development, reproduction, survival, and fitness. Among insect hosts, tephritid fruit flies are well known to form beneficial associations with their symbionts. To control these destructive agricultural pests, environmentally friendly approaches, like the sterile insect technique as a component of integrated pest management strategies, remain most effective. In this study, changes in the bacterial profile of mass‐reared oriental fruit flies, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), were examined in both larval and adult stages and also after irradiation by employing a 16S rRNA gene‐based Illumina sequencing approach. Proteobacteria was the prevalent bacterial phylum in non‐irradiated adults and larvae. Alphaproteobacteria was the most abundant class in larvae but almost absent in adults, which was dominated by Gammaproteobacteria. Firmicutes were present in both developmental stages but at lower relative abundance. At genus level, Acetobacter prevailed in the larval stage and members of the Enterobacteriaceae family in adults. Irradiated samples exhibited higher diversity and richness indices compared to the non‐irradiated oriental fruit flies, whereas no significant changes were observed between the two developmental stages of the non‐irradiated samples. Lactobacillus, members of the Orbacecae family, and Morganella were detected but to a lesser degree upon irradiation, whereas the relative abundance of Lactococcus and Orbus increased. The bacterial profile of larvae appeared to be different compared to that of adult B. dorsalis flies. The subsequent application of irradiation at the pupal stage led to the development of different microbiota between treated and untreated samples, affecting diversity and operational taxonomic unit composition. Irradiated samples of oriental fruit flies were characterized by higher species diversity and richness.

show abstract

“…The gut microbiota provides nutrients to the host, such as amino acids, essential vitamins, carbon, and nitrogen compounds [20,21]. Enterobacteriaceae species in the digestive tract of fruit flies are involved in nitrogen fixation [22], temporal host range expansion [23], reproductive success [21], detoxification [24], protection against pathogens and promoting host immunity [25]. The addition of the gut bacterial species K. oxytoca to the postirradiation diet resulted in improved sterile male competitiveness in Mediterranean fruit flies, and colonization of this strain in the host gut functions to suppress the proliferation of pathogenic strain Pseudomonas [26].…”

mentioning

confidence: 99%

Gut microbiota promotes host resistance to low-temperature stress by stimulating its arginine and proline metabolism pathway in adult Bactrocera dorsalis

et al. 2020

View full text Add to dashboard Cite

Gut symbiotic bacteria have a substantial impact on host physiology and ecology. However, the contribution of gut microbes to host fitness during long-term low-temperature stress is still unclear. This study examined the role of gut microbiota in host low-temperature stress resistance at molecular and biochemical levels in the oriental fruit fly Bactrocera dorsalis. The results showed that after the gut bacteria of flies were removed via antibiotic treatment, the median survival time was significantly decreased to approximately 68% of that in conventional flies following exposure to a temperature stress of 10˚C. Furthermore, we found that Klebsiella michiganensis BD177 is a key symbiotic bacterium, whose recolonization in antibiotic treated (ABX) flies significantly extended the median survival time to 160% of that in the ABX control, and restored their lifespan to the level of conventional flies. Notably, the relative levels of proline and arginine metabolites were significantly downregulated by 34and 10-fold, respectively, in ABX flies compared with those in the hemolymph of conventional flies after exposure to a temperature stress of 10˚C whereas recolonization of ABX flies by K. michiganensis BD177 significantly upregulated the levels of proline and arginine by 13-and 10-fold, respectively, compared with those found in the hemolymph of ABX flies. qPCR analysis also confirmed that K. michiganensis-recolonized flies significantly stimulated the expression of transcripts from the arginine and proline metabolism pathway compared with the ABX controls, and RNAi mediated silencing of two key genes Pro-C and ASS significantly reduced the survival time of conventional flies, postexposure low-temperature stress. We show that microinjection of L-arginine and L-proline into ABX flies significantly increased their survival time following exposure to temperature stress of 10˚C. Transmission electron microscopy (TEM) analysis further revealed that low-temperature stress caused severe destruction in cristae structures and thus resulted in abnormal circular shapes of mitochondria in ABX flies gut, while the recolonization of live K. michiganensis helped the ABX flies to maintain mitochondrial functionality to a normal status, which is important for the arginine and proline induction. Our results suggest that gut microbiota plays a vital role PLOS PATHOGENS PLOS Pathogens | https://doi.

show abstract

Assessing the effects of gut bacteria manipulation on the development of the oriental fruit fly, Bactrocera dorsalis (Diptera; Tephritidae)

Cited by 45 publications

References 37 publications

Integrated LC–MS and GC–MS-based untargeted metabolomics studies of the effect of azadirachtin on Bactrocera dorsalis larvae

Integrated LC–MS and GC–MS-based untargeted metabolomics studies of the effect of azadirachtin on Bactrocera dorsalis larvae

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Gut microbiota promotes host resistance to low-temperature stress by stimulating its arginine and proline metabolism pathway in adult Bactrocera dorsalis

Contact Info

Product

Resources

About