Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses

Mirando, Adam C.; Abdi, Khadar H.; Wo, Peibin; Lounsbury, Karen M.

doi:10.1016/j.ymeth.2016.11.007

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Further studies are warranted to determine the connection between human colon cancer and plasma metabolomic profiles of the citrate cycle pathway in the context of obesity. Second, arginine biosynthesis and aminoacy l- tRNA biosynthesis are two pathways, which are involved in endothelial cell migration and angiogenesis in tumorigenesis [ 38 , 39 ]. However, there are scant data examining plasma metabolomic profiling in this regard.…”

Section: Discussionmentioning

confidence: 99%

Azoxymethane Alters the Plasma Metabolome to a Greater Extent in Mice Fed a High-Fat Diet Compared to an AIN-93 Diet

et al. 2021

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Consumption of a high-fat diet (HFD) links obesity to colon cancer in humans. Our data show that a HFD (45% energy fat versus 16% energy fat in an AIN-93 diet (AIN)) promotes azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) formation in a mouse cancer model. However, the underlying metabolic basis remains to be determined. In the present study, we hypothesize that AOM treatment results in different plasma metabolomic responses in diet-induced obese mice. An untargeted metabolomic analysis was performed on the plasma samples by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). We found that 53 of 144 identified metabolites were different between the 4 groups of mice (AIN, AIN + AOM, HFD, HFD + AOM), and sparse partial least-squares discriminant analysis showed a separation between the HFD and HFD + AOM groups but not the AIN and AIN + AOM groups. Moreover, the concentrations of dihydrocholesterol and cholesterol were inversely associated with AOM-induced colonic ACF formation. Functional pathway analyses indicated that diets and AOM-induced colonic ACF modulated five metabolic pathways. Collectively, in addition to differential plasma metabolomic responses, AOM treatment decreases dihydrocholesterol and cholesterol levels and alters the composition of plasma metabolome to a greater extent in mice fed a HFD compared to the AIN.

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Section: Discussionmentioning

confidence: 99%

Azoxymethane Alters the Plasma Metabolome to a Greater Extent in Mice Fed a High-Fat Diet Compared to an AIN-93 Diet

et al. 2021

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“…TARS is an angiogenesis‐regulating factor as it stimulates the migration of vascular endothelial cells and promotes the formation of blood vessel. 35 Previous studies reported that TARS overexpression was positively correlated with the stage of ovarian cancer 36 and pancreatic cancer cell migration. 37 Our data confirmed elevated TARS expression in GC tissues and paralleled to expression level of VEGFR, an important angiogenic signaling molecule.…”

Section: Discussionmentioning

confidence: 98%

Contribution of upregulated aminoacyl‐tRNA biosynthesis to metabolic dysregulation in gastric cancer

Gao

Guo

et al. 2021

J of Gastro and Hepatol

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Background and Aim: Metabolic reprogramming is characterized by dysregulated levels of metabolites and metabolic enzymes. Integrated metabolomic and transcriptomic data analysis can help to elucidate changes in the levels of metabolites and metabolic enzymes, screen the core metabolic pathways, and develop novel therapeutic strategies for cancer. Methods: Here, the metabolome of gastric cancer tissues was determined using liquid chromatography-mass spectrometry. The transcriptome data from The Cancer Genome Atlas dataset were integrated with the liquid chromatography-mass spectrometry data to identify the common dysregulated gastric cancer-specific metabolic pathways. Additionally, the protein expression and clinical significance of key metabolic enzymes were examined using a gastric cancer tissue array. Results: Metabolomic analysis of 16 gastric cancer tissues revealed that among the 15 dysregulated metabolomic pathways, the aminoacyl-tRNA biosynthesis pathway in the gastric tissues was markedly upregulated relative to that in the adjacent noncancerous tissues, which was consistent with the results of transcriptome analysis. Bioinformatic analysis revealed that among the key regulators in the aminoacyl-tRNA biosynthesis pathway, the expression levels of threonyl-tRNA synthetase (TARS) and phenylalanyl-tRNA synthetase (FARSB) were correlated with tumor grade and poor survival, respectively. Additionally, gastric tissue array data analysis indicated that TARS and FARSB were upregulated in gastric cancer tissues and were correlated with poor prognosis and tumor metastasis. Conclusions: This study demonstrated that the aminoacyl-tRNA biosynthesis pathway is upregulated in gastric cancer and both TARS and FARSB play key roles in the progression of gastric cancer. Additionally, a novel therapeutic strategy for gastric cancer was proposed that involves targeting the aminoacyl-tRNA biosynthesis pathway.

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“…Moreover, polymyositis/dermatomyositis (PM/DM), which is also related to the ThrRS mutation, is usually complicated by interstitial lung disease that seriously impacts the patient's life 32 . Furthermore, ThrRS also engages in angiogenesis 33 . The zebrafish mutant presents disorganized vessels with abnormal branching of the intersegmental vessels and an aberrant brain vascular network 34 .…”

Section: Introductionmentioning

confidence: 99%

“…32 Furthermore, ThrRS also engages in angiogenesis. 33 The zebrafish mutant presents disorganized vessels with abnormal branching of the intersegmental vessels and an aberrant brain vascular network. 34 Its overexpression is correlated with progression of human ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Threonyl‐tRNA synthetase gene, a potential target for RNAi‐based control of three rice planthoppers

Zhang

et al. 2022

Pest Management Science

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Background RNA interference (RNAi) has potential as a new strategy for pest control. However, the current overemphasis on the control of a single pest increased control costs. The aim of this study was to find a green method of controlling several pests without affecting the natural enemies with a single target gene. One possible RNAi target is the threonyl‐tRNA synthetase (ThrRS), which is conserved and plays a significant role in protein biosynthesis. Results In this study, one threonyl‐tRNA synthetase gene (NlthrS) was identified from the brown planthopper (Nilaparvata lugens). Spatio‐temporal expression pattern analysis showed that NlthrS was highly expressed in the ovary, late embryogenesis, nymphs and female adults. In addition, RNAi‐mediated knockdown of NlthrS caused 85.6% nymph mortality, 100% female infertility, molting disorder, extended nymph duration and shortened adult longevity. Target‐specific results were obtained when dsNlthrS was used to interfere with the whiteback planthopper (Sogatella furcifera), small brown planthopper (Laodelphax striatellus), zig‐zag winged leafhopper (Inazuma dorsalis) and their natural enemy (green mirid bug, Cyrtorhinus lividipennis). In addition, dsNlthrS could cause high mortalities of three species of planthoppers (85.6–100%), while only dsNlthrS‐1 led to the death (97.3%) of I. dorsalis that was not affected by dsNlthrS‐2. Furthermore, neither dsNlthrS‐1 nor dsNlthrS‐2 could influence the survival of C. lividipennis. Conclusion The results reveal the biological functions of ThrRS in N. lugens in addtion to its protein synthesis, deepening our understanding of tRNA synthase in insects and providing a new method for the control of several rice pests via one dsRNA design. © 2022 Society of Chemical Industry.

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Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses

Cited by 6 publications

References 21 publications

Azoxymethane Alters the Plasma Metabolome to a Greater Extent in Mice Fed a High-Fat Diet Compared to an AIN-93 Diet

Azoxymethane Alters the Plasma Metabolome to a Greater Extent in Mice Fed a High-Fat Diet Compared to an AIN-93 Diet

Contribution of upregulated aminoacyl‐tRNA biosynthesis to metabolic dysregulation in gastric cancer

Threonyl‐tRNA synthetase gene, a potential target for RNAi‐based control of three rice planthoppers

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