2020
DOI: 10.3791/61436
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Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Abstract: Increasing evidence supports the hypothesis that neuro-immune interactions impact nervous system function in both homeostatic and pathologic conditions. A well-studied function of major histocompatibility complex class I (MHCI) is the presentation of cell-derived peptides to the adaptive immune system, particularly in response to infection. More recently it has been shown that the expression of MHCI molecules on neurons can modulate activity-dependent changes in the synaptic connectivity during normal developm… Show more

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Cited by 3 publications
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“…Primary cortical neurons were isolated from embryos collected from timed-pregnant C57BL/6 mice on embryonic day 18 as previously described (Funk and Lotz, 2020). Briefly, cerebral cortices were dissected from embryonic brains, treated with papain (20 U/mL) and DNase 1 (2.5 U/mL) for 30 minutes, and gently dissociated by trituration in Hibernate E medium (Life Technologies, cat#A1247601).…”
Section: In Vitro Cd8 + T Cell Neuron Co-culturesmentioning
confidence: 99%
“…Primary cortical neurons were isolated from embryos collected from timed-pregnant C57BL/6 mice on embryonic day 18 as previously described (Funk and Lotz, 2020). Briefly, cerebral cortices were dissected from embryonic brains, treated with papain (20 U/mL) and DNase 1 (2.5 U/mL) for 30 minutes, and gently dissociated by trituration in Hibernate E medium (Life Technologies, cat#A1247601).…”
Section: In Vitro Cd8 + T Cell Neuron Co-culturesmentioning
confidence: 99%