Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum

Ni, Ke; Zhao, Jing-Yun; Bao-cheng, Zhu; Su, Rongguo; Pan, Yi; Jun-kui, MA; Guo, Ziyi; Tao, Yong; Liu, Xiaorong; Zhong, Jin

doi:10.1016/j.biortech.2018.05.097

Cited by 179 publications

(150 citation statements)

References 13 publications

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“…The results from the current study indicate that alfalfa silage had increased pH and ammonia N concentrations and decreased lactic acid concentrations compared to orchardgrass silage and silage mixtures of orchardgrass and alfalfa showed suitable ensiling characteristics and high associative effects indices. In accordance with previous studies [41,42], silage mixtures could be a feasible way to preserve nutrition and improve fermentation quality through the role of dominated Lactobacillus, compared with the sole silage crops.…”

Section: Ensiling Characteristics Of Orchardgrass and Alfalfa Silage supporting

confidence: 88%

Combining Orchardgrass and Alfalfa: Effects of Forage Ratios on In Vitro Rumen Degradation and Fermentation Characteristics of Silage Compared with Hay

Xue

Wang

et al. 2019

Animals

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Simple Summary: Forages are an essential portion of ruminant rations to maintain rumen function. Exploring how orchardgrass and alfalfa interact in the rumen is necessary to better understand their feed use potential as both hay and silage. This study evaluated in vitro rumen degradation, fermentation characteristics, and methane production responses to different forage ratios of alfalfa and orchardgrass. The results indicate that dry matter and organic matter degradability and methane production were greater for mixed silages compared to mixed hays. A forage ratio of 50:50 for orchardgrass and alfalfa favor the growth of rumen microorganisms without compromising nutrient digestion and rumen fermentation.Abstract: This study aimed to investigate the effects of different forage ratios of orchardgrass (Dactylis glomerata) and alfalfa (Medicago sativa) on in vitro rumen degradation and fermentation characteristics. Orchardgrass and alfalfa were harvested separately and prepared as hay and silage mixtures at ratios of 100:0, 75:25, 50:50, 25:75, and 0:100 (w/w on a dry matter basis) and anaerobically incubated for 48 h with rumen fluid obtained from lactating dairy cows. Fermented residues and cultured fluids were used to determine nutrient degradability, fermentation parameters, and associative effect indices. Increasing the proportion of alfalfa in hay and silage mixtures quadratically increased in vitro organic matter disappearance (IVOMD, up +5.14%) and marginally decreased in vitro neutral detergent fiber disappearance (NDFD, down −1.79%). Meanwhile, increasing the proportion of alfalfa accelerated the rumen fermentation process (e.g., gas production) and remarkably enhanced the growth of rumen microbes as indicated by microbial protein production (MCP, 13.4% increase). Increments of rumen degradability and methane production were more pronounced in silage mixtures than hay mixtures. In combination, a forage ratio of 50:50 for orchardgrass and alfalfa is recommended for both hay and silage in order to improve the feed use potential in ruminants.

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Section: Ensiling Characteristics Of Orchardgrass and Alfalfa Silage supporting

confidence: 88%

Combining Orchardgrass and Alfalfa: Effects of Forage Ratios on In Vitro Rumen Degradation and Fermentation Characteristics of Silage Compared with Hay

Xue

Wang

et al. 2019

Animals

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“…The clear separation between bacterial communities of the ensiling material and alfalfa silage indicated a shift after ensiling, consistent with previous studies (Ni et al, 2017b;Yang et al, 2019). Divisions between the plots representing silage with and without inoculants indicated that the distribution of the bacterial community was shifted by L. plantarum inoculations, which could explain the differences in fermentation quality (Ni et al, 2018). The distribution of the bacterial communities among the three replications within LPII was more similar compared with those within LPI for both periods.…”

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Sisupporting

confidence: 88%

“…The 24-h wilting may have exacerbated the lack of WSC (17.56 g/kg DM) in this study. The WSC of the ensiling material was lower than 50 g/kg DM, which was insu cient for adequate fermentation during ensiling (Ni et al, 2018). The BC was still high after wilting in this study.…”

Section: Characteristics Of Wilted Alfalfamentioning

confidence: 56%

“…Acceleration of the LA fermentation with L. plantarum inoculation was also shown at the genus level. The major bacteria involved in LA fermentation of silage belong to the genera Lactobacillus, Pedicoccus, Weissella, and Leuconostoc (Ni et al, 2018;Pang et al, 2011). Low abundance of these genera was exhibited in the wilted alfalfa before ensiling, and aerobic microorganisms dominated the bacterial community.…”

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Simentioning

confidence: 99%

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Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

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BackgroundThe objective of this study was to investigate the mechanism of Lactobacillus plantarum (L. plantarum) involved in improving fermentation quality of naturally ensiled alfalfa under poor conditions. ResultsHigh-moisture wilted alfalfa was ensiled without inoculants (CK) or with inoculation of two L. plantarum additives (LPI and LPII). The pH and fermentation products of silage were investigated, and the bacterial community compositions were analyzed. The L. plantarum inoculants significantly enhanced the lactic acid fermentation in terms of promotions in pH decline, lactic acid accumulation, and Lactobacillus abundance for both periods. At 90 d, silage in CK exhibited a high pH, a loss in dry matter, and a high concentration of ammoniacal nitrogen. The inoculations of L. plantarum significantly inhibited the growth of Clostridia, and reduced ammoniacal nitrogen concentration in silage (P < 0.05). Conclusions Inoculation with L. plantarum improved the fermentation quality of alfalfa silage and inhibited the growth of spoilage microorganisms, and further delayed decomposition of alfalfa silage under adverse ensiling conditions.

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“…Alfalfa and stylo samples before ensiling were separated from the silage samples, which suggested that microbial community changed during ensiling process. The variation of microbial community might explain the difference in silage quality (Ni et al , ). Distinctions among bacterial communities in silages mixed with two ratios of NCL were also very clear.…”

Section: Resultsmentioning

confidence: 99%

Effects of mixing Neolamarckia cadamba leaves on fermentation quality, microbial community of high moisture alfalfa and stylo silage

Wang

Xing

et al. 2019

Microbial Biotechnology

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Summary Neolamarckia cadamba is not only a fodder of high nutritional value, but also a source of natural antimicrobial agent. The silage quality of high moisture alfalfa and stylo with or without N. cadamba leaves (NCL) was investigated, and microbial community after ensiling was analysed. Results showed that the silage samples with NCL have lower pH (4.32 versus 4.88, 4.26 versus 4.71 in alfalfa and stylo silage, respectively), ammonia‐N content (67.5 versus 146, 42.2 versus 95.1 g kg−1 total N) and higher lactic acid (13.3 versus 10.4, 17.3 versus 13.6 g kg−1 dry matter), true protein N (592 versus 287, 815 versus 589 g kg−1 total N). The addition of NCL also influenced the bacterial community distribution. The relative abundance of Clostridium and Enterobacter decreased, whereas Lactobacillus abundance increased when NCL was added. In conclusion, NCL could inhibit undesirable microorganisms in high moisture alfalfa and stylo silage. Mixing with NCL could be a feasible way to improve the quality of silage.

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Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum

Cited by 179 publications

References 13 publications

Combining Orchardgrass and Alfalfa: Effects of Forage Ratios on In Vitro Rumen Degradation and Fermentation Characteristics of Silage Compared with Hay

Combining Orchardgrass and Alfalfa: Effects of Forage Ratios on In Vitro Rumen Degradation and Fermentation Characteristics of Silage Compared with Hay

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Effects of mixing Neolamarckia cadamba leaves on fermentation quality, microbial community of high moisture alfalfa and stylo silage

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