Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

Castañeda-Cardona, Christian C.; Morillo-Coronado, Yacenia; Morillo, Ana Cruz

doi:10.4067/s0718-58392020000400608

Cited by 6 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The population II obtained values ranging from 0.50 (GT) to 0.58 (CT), with an average value of 0.54. These data are similar to the ones of previous studies of other allogamous species, such as Chenopodium quinoa (Abd El-Moneim et al, 2021), and Dioscorea alata (Castañeda-Cardona et al, 2020).…”

Section: Resultssupporting

confidence: 92%

Genetic diversity pattern of Passiflora spp. in Boyacá, Colombia

Coronado,

Camargo,

Coronado

2023

Pesq. agropec. bras.

View full text Add to dashboard Cite

The objective of this work was to characterize the genetic diversity, using ISSR markers, of 70 genotypes of five species of Passiflora spp. in Boyacá, Colombia. For molecular characterization, samples of young leaves were collected from 11 municipalities of the Boyacá department. Genetic similarity was used to cluster the genotypes by the UPGMA method, and genetic structure was evaluated by the Bayesian model. Eight ISSR primers produced 138 loci. The formed cluster consists of two populations, with most individuals of the same species but from different geographic origins. The percentage of polymorphic loci is higher than 80%. The average value of heterozygosity is between 0.29 and 0.36 for population I and II, respectively, and the values of polymorphic information content are low. A moderate genetic differentiation (0.16) and high gene flow (3.35) are observed.

show abstract

Section: Resultssupporting

confidence: 92%

Genetic diversity pattern of Passiflora spp. in Boyacá, Colombia

Coronado,

Camargo,

Coronado

2023

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…groups might be used for conservation and breeding of this species. Similar results have been reported in other studies of genetic diversity in other fruit species using ISSR markers (Morillo et al, 2017;Castañeda-Cardona et al, 2020;Martínez et al, 2020).…”

Section: Discussionsupporting

confidence: 91%

Analysis of the genetic diversity of Dragon fruit based on ISSR markers in Colombia

2022

View full text Add to dashboard Cite

Selenicereus megalanthus H. is a tropical fruit belonging to the family Cactaceae, is rich in essential nutrients, antioxidants and bioactive components. It presents wide variability in different characteristics and a great demand in the market; however, genetic studies in Colombia are scarce. The main of this study was to characterize the genetic diversity of 76 yellow pitahaya genotypes with eight ISSR markers. Genetic parameters expected average heterozygosity (He), percentage of polymorphic loci, genetic distances and Fst were estimated with TFPGA. The analysis of the population genetic structure was carried out with the STRUCTURE 2.3.4. As a result, 225 alleles were generated and the number of polymorphic loci ranged 85 (CT, AG) to 90 (GT). High genetic diversity was found, with an average value of heterozygosity was 0.34 with a genetic differentiation coefficient (Fst) of 0.26, indicating that there was a great genetic diversity, similar values than those reported in other studies of pitahaya genetic diversity in Colombia. The 76 genotypes were grouped into K=3 according to geographic location, however, in some groups a mixture of individuals from different origins was observed. The analysis of molecular variance (AMOVA) showed higher variation (75%) within groups than among groups (25%). These results provide information that can be used to develop conservation strategies for dragon fruit and breeding programs to obtain more productive pitahaya genotypes with superior quality, high yield and with resistance to biotic and abiotic factors.

show abstract

“…High polymorphism (83.62%) was also observed in studies on Dioscorea spp. (Castañeda-Cardona et al 2020) in assessment of the genetic diversity of 21 local strains of yam from China using ISSR primers (93.5%) (Wu et al 2014), and 92% of the loci were polymorphic in a study of the genetic diversity of D. alata L. using SSR primers (Massawe and Temu 2023).…”

Section: Discussionmentioning

confidence: 99%

“…SSR markers have been used for genetic studies of yam germplasm, as they are considered informative and show high discriminatory power (Loko et al 2017;Girma et al 2017;Chen et al 2022;Kalu et al 2022). The ISSR markers also allowed detection of a high level of polymorphism for the Dioscorea alata L. species (83.62%) (Castañeda-Cardona et al 2020) and for 21 local strains of Dioscorea spp. (93.5%) from China (Wu et al 2014) Therefore, considering the social and economic importance of the yam crop and the need for more information regarding the genetic resources of yam generated by farmers, the aim of this study was to evaluate the genetic diversity of genotypes of Dioscorea sp.…”

mentioning

confidence: 99%

Genetic diversity of yam (Dioscorea sp.) through morphological and molecular markers

Santos,

Gois,

Prata

et al. 2024

Preprint

View full text Add to dashboard Cite

The aim of the present study was to evaluate the genetic diversity of genotypes of Dioscorea sp. originating from states of the Brazilian Northeast region (Sergipe, Bahia, Alagoas, and Paraíba) through morphological and molecular characterization. For this evaluation, 52 yam genotypes were collected from market locations and directly from rural growers. Morphological characterization made use of 31 morphological descriptors for the yam crop. The environmental coefficient of variation (CVe) of most of the descriptors evaluated was greater than the genotypic coefficient of variation (CVg), and heritability was high only for stem color (86.86%). The highest significant positive correlation was between the presence of the tuber and the presence of roots on the surface of the tuber (r = 1; p < 0.001). The genotypes were clustered in seven different groups based on the morphological descriptors evaluated. For molecular characterization, SSR (9) and ISSR (16) primers were used. The primers used were considered informative, showing high polymorphic information content; and 100% of the bands were polymorphic. Genetic diversity was estimated by the following parameters: number of alleles (2.00), effective number of alleles (1.30), expected heterozygosity (0.211), and the Shannon index (0.351). The 52 genotypes were clustered in seven groups according to the Jaccard distance. The genetic structure of the population in K = 2 reveals shared alleles among the genotypes of different geographic origins. Morphological and molecular characterization reveals the high genetic diversity among the 52 genotypes of Dioscorea sp. and low diversity among the collection sites.

show abstract

Assessing the genetic diversity of Dioscorea alata and related species from Colombia through inter-simple sequence repeat (ISSR) markers

Cited by 6 publications

References 23 publications

Genetic diversity pattern of Passiflora spp. in Boyacá, Colombia

Genetic diversity pattern of Passiflora spp. in Boyacá, Colombia

Analysis of the genetic diversity of Dragon fruit based on ISSR markers in Colombia

Genetic diversity of yam (Dioscorea sp.) through morphological and molecular markers

Contact Info

Product

Resources

About