Assessing the genotoxicity and carcinogenicity of 2-chloroethanol through structure activity relationships and in vitro testing approaches

Allemang, Ashley; Lester, Cathy; Roth, Thomas; Pfuhler, Stefan; Peuschel, Henrike; Kosemund, Kirstin; Mahony, Catherine; Bergeland, Trygve; O’Keeffe, Lara

doi:10.1016/j.fct.2022.113290

Cited by 4 publications

(2 citation statements)

References 55 publications

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“…This is also the case with ethylene oxide content. Reports published in the RASFF database of exceedances of ethylene oxide levels in sesame seeds resulted in increased control of this parameter [1,12].…”

Section: Resultsmentioning

confidence: 99%

A Fast Method for Determination of Ethylene Oxide Using Gas Chromatography Coupled with Mass Spectrometry GC-MS/MS

Wenio,

Bartosiewicz,

Derewiaka

et al. 2023

Applied Sciences

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The ongoing influx of information regarding the presence of ethylene oxide in food requires the implementation of a simple and quick analytical procedure. This paper propounds one such effective procedure for the analysis of ethylene oxide (EtO) bactericides by GC-MS/MS in food. The presented method is based on the derivatization of ethylene oxide to 2-chloroethanol, with both the number of extraction steps and the volume of extraction solvent having been minimized. The purification of the final extract through a syringe filter has proved to significantly reduce the amount of recovery obtained. Since the definition of residue pesticide ethylene oxide, according to the directive 868/2015, states that ethylene oxide is the sum of ethylene oxide and 2-chloroethanol expressed as ethylene oxide, the validated method allows for the determination of this definition. The presented method has turned out to be faster than other methods and it complies with the latest European guidelines for the analysis of pesticide residues outlined in the SANTE/11312/2021 document. Linearity, limits of detection and quantification, matrix effects, accuracy, and precision of the method have been assessed with respect to the above-mentioned document. Expanded uncertainty RSDr was less than or equal to 20%, while the recovery achieved oscillated within the range of 70–120%. The laboratory has been accredited for using the described procedure of the chromatographic method, the competence of which has been confirmed by numerous proficiency tests.

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Section: Resultsmentioning

confidence: 99%

A Fast Method for Determination of Ethylene Oxide Using Gas Chromatography Coupled with Mass Spectrometry GC-MS/MS

Wenio,

Bartosiewicz,

Derewiaka

et al. 2023

Applied Sciences

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“…The ToxTracker assay is typically applied as (i) a screening assay for early in vitro genotoxicity prediction or (ii) a follow‐up test to assess MoA for a compound with positive or equivocal test results from a regulatory accepted in vitro and/or in vivo genotoxicity assay (Allemang et al, 2022; Mesnage et al, 2021; Smart et al, 2022). ToxTracker fits well with the Clean Sheet approach that was proposed by the Health and Environmental Sciences Institute (HESI) Genetic Toxicology Technical Committee (GTTC) (Dearfield et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Interlaboratory validation of the ToxTracker assay: An in vitro reporter assay for mechanistic genotoxicity assessment

Hendriks,

Adriaens,

Allemang

et al. 2024

Environ and Mol Mutagen

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ToxTracker is a mammalian cell reporter assay that predicts the genotoxic properties of compounds with high accuracy. By evaluating induction of various reporter genes that play a key role in relevant cellular pathways, it provides insight into chemical mode‐of‐action (MoA), thereby supporting discrimination of direct‐acting genotoxicants and cytotoxic chemicals. A comprehensive interlaboratory validation trial was conducted, in which the principles outlined in OECD Guidance Document 34 were followed, with the primary objectives of establishing transferability and reproducibility of the assay and confirming the ability of ToxTracker to correctly classify genotoxic and non‐genotoxic compounds. Reproducibility of the assay to predict genotoxic MoA was confirmed across participating laboratories and data were evaluated in terms of concordance with in vivo genotoxicity outcomes. Seven laboratories tested a total of 64 genotoxic and non‐genotoxic chemicals that together cover a broad chemical space. The within‐laboratory reproducibility (WLR) was up to 98% (73%–98% across participants) and the overall between‐laboratory reproducibility (BLR) was 83%. This trial confirmed the accuracy of ToxTracker to predict in vivo genotoxicants with a sensitivity of 84.4% and a specificity of 91.2%. We concluded that ToxTracker is a robust in vitro assay for the accurate prediction of in vivo genotoxicity. Considering ToxTracker's robust standalone accuracy and that it can provide important information on the MoA of chemicals, it is seen as a valuable addition to the regulatory in vitro genotoxicity battery that may even have the potential to replace certain currently used in vitro battery assays.

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Indirubin mediates adverse intestinal reactions in guinea pigs by downregulating the expression of AchE through AhR

Xu,

Taha,

Chu

et al. 2024

Xenobiotica

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Assessing the genotoxicity and carcinogenicity of 2-chloroethanol through structure activity relationships and in vitro testing approaches

Cited by 4 publications

References 55 publications

A Fast Method for Determination of Ethylene Oxide Using Gas Chromatography Coupled with Mass Spectrometry GC-MS/MS

A Fast Method for Determination of Ethylene Oxide Using Gas Chromatography Coupled with Mass Spectrometry GC-MS/MS

Interlaboratory validation of the ToxTracker assay: An in vitro reporter assay for mechanistic genotoxicity assessment

Indirubin mediates adverse intestinal reactions in guinea pigs by downregulating the expression of AchE through AhR

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