Assessing the impact of curcumin on dual‐species biofilms formed by <i>Streptococcus mutans</i> and <i>Candida albicans</i>

Li, Xinlong; Yin, Luoping; Ramage, Gordon; Li, Bingchun; Tao, Ye; Zhi, Qinghui; Lin, Huancai; Zhou, Yan

doi:10.1002/mbo3.937

Cited by 55 publications

(43 citation statements)

References 35 publications

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“…In turn, Alalwan et al [ 48 ] observed its anti-adhesive effects against C. albicans biofilm formation at the sub-inhibitory concentration of MIC/2. The antibiofilm properties of curcumin have also been reported for various bacterial species, including A. baumannii [ 42 ], E. faecalis [ 39 ], E. coli , P. aeruginosa , P. mirabilis , S. marcescens [ 24 ], S. epidermidis [ 49 ], and S. mutans [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

2020

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Curcumin, a principal bioactive substance of turmeric (Curcuma longa L.), is reported as a strong antioxidant, anti-inflammatory, antibacterial, antifungal, and antiviral agent. However, its antimicrobial properties require further detailed investigations into clinical and multidrug-resistant (MDR) isolates. In this work, we tested curcumin’s efficacy against over 100 strains of pathogens belonging to 19 species. This activity was determined by the broth microdilution method and by calculating the minimum inhibitory concentration (MIC). Our findings confirmed a much greater sensitivity of Gram-positive than Gram-negative bacteria. This study exhibited a significantly larger variation in the curcumin activity than previous works and suggested that numerous clinical strains of widespread pathogens have a poor sensitivity to curcumin. Similarly, the MICs of the MDR types of Staphylococcus aureus, S. haemolyticus, Escherichia coli, and Proteus mirabilis were high (≥2000 µg/mL). However, curcumin was effective against some species and strains: Streptococcus pyogenes (median MIC = 31.25 µg/mL), methicillin-sensitive S. aureus (250 µg/mL), Acinetobacter lwoffii (250 µg/mL), and individual strains of Enterococcus faecalis and Pseudomonas aeruginosa (62.5 µg/mL). The sensitivity of species was not associated with its affiliation to the genus, and it could differ a lot (e.g., S. pyogenes, S. agalactiae and A. lwoffii, A. baumannii). Hence, curcumin can be considered as a promising antibacterial agent, but with a very selective activity.

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Section: Discussionmentioning

confidence: 99%

Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

2020

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“…The resulting samples were mounted on aluminum cylinders containing carbon strips and evaporated under high vacuum (Denton Vacuum Desk V; Jeol Ltd., Moorestown, NJ, United States) for gold coating. The topographical characteristics of the biofilms were analyzed by electron microscopy (JSM-6610 Series Scanning Electron Microscope; Jeol Ltd., United States) at the School of Dentistry of UNESP at Araraquara, SP, Brazil (Martinez et al, 2010;Costa-Orlandi et al, 2014;Li et al, 2019).…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

A Two-Way Road: Antagonistic Interaction Between Dual-Species Biofilms Formed by Candida albicans/Candida parapsilosis and Trichophyton rubrum

et al. 2020

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Dermatomycoses include superficial fungal infections of the skin and its appendages. Trichophyton rubrum, Candida albicans, and Candida parapsilosis are some of the most prevalent species that cause dermatomycoses. Several studies show a variable predominance of Candida spp. in relation to dermatophytes, especially in onychomycosis and the possibility of isolating both from the same site. The ability of dermatophytes to form biofilms recently been explored and there is currently no evidence on the involvement of these filamentous fungi in multi-species biofilms. Thus, this study aims to investigate the probable dual-species interaction between T. rubrum and C. albicans and T. rubrum and C. parapsilosis biofilms, considering variable formation conditions, as well as the susceptibility of these dual-species biofilms against terbinafine and efinaconazole. Three conditions of formation of dual-species biofilms were tested: (a) the suspensions of T. rubrum and Candida albicans or C. parapsilosis placed together; (b) suspensions of C. albicans and C. parapsilosis added the preadhesion of T. rubrum biofilms; (c) after the maturation of T. rubrum sessile cells. In the first and second conditions, the quantification of metabolic activities, biomass, and polysaccharide materials of mixed biofilms tended to resemble Candida monospecies biofilms. In the third condition, the profiles were modified after the addition of Candida, suggesting that T. rubrum biofilms served as substrate for the development of Candida biofilms. Scanning electron microscopy showed Candida predominance, however, numerous blastoconidia were noted, most evident in the conditions under which Candida was added after the pre-adhesion and maturation of T. rubrum biofilms. Despite the predominance of Candida, the presence of T. rubrum appears to inhibit C. albicans filamentation and C. parapsilosis development, confirming an antagonistic interaction. Fungal burden assays performed when the biofilms were formed together confirmed Candida predominance, as well as susceptibility to antifungals. Further studies will be needed to identify the components of the Candida and T. rubrum biofilm supernatants responsible for inhibiting dermatophyte growth and C. albicans filamentation.

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“…The inhibitory effects of chelerythrine on the biofilm formation were observed by field emission scanning electron microscopy (FESEM; Nova Nano SEM-450, FEI, Hillsboro, OR, USA) and confocal laser scanning microscopy (CLSM; Zeiss LSM 880 with Airyscan), as described previously [21]. Approximately 1 × 10 6 CFU/mL of mono-species and dual-species were cultured on the glass coverslips in individual wells of a 24-well microtiter plate, and treated with chelerythrine at 0, 1/8, 1/4, and 1/2 MIC at 37 • C for 24 h. For FESEM analysis, followed by treatment, the glass coverslips with biofilms were kept in sterile water containing 2.5% glutaraldehyde (v/v; Sigma-Aldrich, USA) at −4 • C for 2 h. Then, biofilms were rinsed three times with 10 mM PBS (pH 7.0) and dehydrated in water-alcohol solutions at C T E D various alcohol concentrations of 30%, 50%, 70%, 90%, and 100% for 10 min each.…”

Section: Biofilm Inhibition Assaymentioning

confidence: 99%

RETRACTED: Efficacy of Chelerythrine against Mono- and Dual-Species Biofilms of Candida albicans and Staphylococcus aureus and Its Properties of Inducing Hypha-to-Yeast Transition of C. albicans

Qian

Zhang

Wang

et al. 2020

JoF

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Candida albicans and Staphylococcus aureus specifically often resulted in biofilm-associated diseases, ranging from superficial mucosal to life-threatening systemic infections. Recent studies reported that chelerythrine displayed antimicrobial activities against a few microorganisms, but its effects on mono- and dual-species biofilms of C. albicans and S. aureus have never been reported. The purpose of this study was to evaluate the efficacy of chelerythrine against mono- and dual-species biofilms, and explore its effect on the hyphal growth and the hypha-to-yeast transition of C. albicans. The results showed that minimum inhibitory concentrations (MICs) and minimum biofilm inhibitory concentration (MBIC90S) of chelerythrine against planktonic cells of mono-species were 4 and 2 μg/mL, while the MIC and MBIC90 were 6 and 3 μg/mL for dual-species. Meanwhile, the decrease in three matrix component levels and tolerance to antibiotics of biofilms formed by mono- and dual-species exposed to chelerythrine were confirmed by a confocal laser scanning microscope, in conjugation with five fluorescent dyes and a gatifloxacin diffusion assay. Moreover, C. albicans and S. aureus mono-species showed a 96.4, and 92.3% reduction, respectively, in 24-h preformed biofilm biomass in the presence of 128 µg/mL of chelerythrine. Similarly, preformed (24 h) dual-species biofilm biomass also displayed a significant reduction (90.7%) when treated with 192 μg/mL chelerythrine. Chelerythrine inhibited hyphae formation of C. albicans at 4 μg/mL, and C. albicans in hypha-form can be converted into yeast-form at 8 μg/mL of chelerythrine. Therefore, chelerythrine shows promise as a potential antimicrobial and antibiofilm agent for clinical effective treatments of mono- and mixed-species and/or biofilm-associated infections.

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Assessing the impact of curcumin on dual‐species biofilms formed by Streptococcus mutans and Candida albicans

Cited by 55 publications

References 35 publications

Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

Curcumin, a Natural Antimicrobial Agent with Strain-Specific Activity

A Two-Way Road: Antagonistic Interaction Between Dual-Species Biofilms Formed by Candida albicans/Candida parapsilosis and Trichophyton rubrum

RETRACTED: Efficacy of Chelerythrine against Mono- and Dual-Species Biofilms of Candida albicans and Staphylococcus aureus and Its Properties of Inducing Hypha-to-Yeast Transition of C. albicans

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