Assessing the impact of the biological control agent<i>Bacillus thuringiensis</i>on the indigenous microbial community within the pepper plant phyllosphere

Zhang, Baoguo; Bai, Zhihui; Hoefel, Daniel; TANG, Ling; Yang, Zhiguang; Zhuang, Guoqiang; Yang, Jianghai; Zhang, Hongxun

doi:10.1111/j.1574-6968.2008.01178.x

Cited by 50 publications

(38 citation statements)

References 29 publications

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“…Previous studies, using plating and low-throughput molecular techniques, reported that the introduction of a biocontrol agent or a pathogen to the system had a marked impact on the plant microbiome (Teixidó et al, 1998;Zhang et al, 2008;BuddrusSchiemann et al, 2010;Chowdhury et al, 2013;Yin et al, 2013). Erlacher et al (2014) demonstrated shifts in the microbiota of lettuce as a result of introducing a pathogen (Rhizoctonia solani) and/or a biocontrol agent.…”

Section: The Role Of the Microbiome In Fruit Health And Disease à A Nmentioning

confidence: 99%

“…Numerous reports have been published on various strategies and approaches that can be used to enhance the efficacy and reliability of postharvest biocontrol agents. These include combining biocontrol agents with use of salts and organic acids (Droby et al, 1997;Karabulut et al, 2001), glucose analogs (El Ghaouth et al, 2000), food additives Karabulut et al, 2003;Teixidó et al, 2001), and various physical treatments (Karabulut et al, 2002;Porat et al, 2002;Zhang et al, 2008). In most cases, enhanced efficacy was demonstrated using these approaches, however, each commodity-pathogen system has its own unique features and so specific protocols will need to commercially evaluated.…”

Section: Performance and Consistencymentioning

confidence: 99%

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The science, development, and commercialization of postharvest biocontrol products

Droby

Wisniewski

Teixidó

et al. 2016

Postharvest Biology and Technology

292

149

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A B S T R A C TPostharvest biological control agents as a viable alternative to the use of synthetic chemicals have been the focus of considerable research for the last 30 years by many scientists and several commercial companies worldwide. Several antagonists of postharvest pathogens have been identified and tested in laboratory, semi-commercial, and commercial settings and were developed into commercial products. The discovery and development of these antagonists into a product followed a paradigm in which a single antagonist isolated from one commodity was also expected to be effective on other commodities that vary in their genetic background, physiology, postharvest handling, and susceptibility to pathogens. In most cases, product development was successfully achieved but their full commercial potential was not realized. The low success rate of postharvest biocontrol products has been attributed to several problems, including difficulties in mass production and formulation of the antagonist, the physiological status of the harvested commodity and its susceptibility to specific pathogens. All these factors played a major role in the reduced and inconsistent performance of the biocontrol product when used under commercial conditions. Although many studies have been conducted on the mode of action of postharvest microbial antagonists, our understanding is still very incomplete. In this regard, a systems approach, that takes into account all the components of the biocontrol system, may represent the best approach to investigating the network of interactions that exist. Very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types, their function, the factors that influence the composition of the microbiota after harvest and during storage, and the distribution of individual taxa. In light of the progress made in recent years in metagenomic technologies, this technology should be used to characterize the composition of microbial communities on fruit and vegetables. Information on the dynamics and diversity of microbiota may be useful to developing a new paradigm in postharvest biocontrol that is based on constructing synthetic microbial communities that provide superior control of pathogens.

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Section: The Role Of the Microbiome In Fruit Health And Disease à A Nmentioning

confidence: 99%

Section: Performance and Consistencymentioning

confidence: 99%

The science, development, and commercialization of postharvest biocontrol products

Droby

Wisniewski

Teixidó

et al. 2016

Postharvest Biology and Technology

292

149

View full text Add to dashboard Cite

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“…Biocontrol agents have been used to protect plants against foliar diseases in several crops (Yuen and Schoneweis, 2007;Alvindia and Natsuaki, 2008;Perello et al, 2007). However, many of them are poor competitors for leaf surface nutrients compared with indigenous phyllosphere microbes (Zhang et al, 2008a;Brandl, 2003, Lindow andLeveau, 2002).…”

Section: Introductionmentioning

confidence: 99%

Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

Gu¹,

Bai²,

Jin³

et al. 2010

Journal of Environmental Sciences

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A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater (MGW) and potato wastewater (PW) as culture medium. The maximum yield of 6.5 × 10 9 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28°C, pH 6.0-7.5 using the mixture of MGW and PW (1:9).

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“…In recent years, various culture-independent methods such as phospholipid fatty acid analysis (PLFA) and 16S rRNA gene directed Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) have been useful for detecting pollutant-induced changes in the composition of natural microbial communities (Morimoto et al, 2005;Chinalia and Killham, 2006;Ratcliff et al, 2006). PLFA results are interpreted based on the assumption that phospholipids make up a relatively constant proportion of the cell biomass and that variation in fatty acids among taxonomic groups can be used as biomarkers and the PLFA pattern of an environmental sample therefore reflects the microbial community composition (White et al, 1996;Zhang et al, 2008). PCR-DGGE separates 16S rRNA gene fragments based on differences to their resistance to chemical denaturation, generating a genetic profile of the individual bacterial species within a community (Burr et al, 2006;Zhang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…PLFA results are interpreted based on the assumption that phospholipids make up a relatively constant proportion of the cell biomass and that variation in fatty acids among taxonomic groups can be used as biomarkers and the PLFA pattern of an environmental sample therefore reflects the microbial community composition (White et al, 1996;Zhang et al, 2008). PCR-DGGE separates 16S rRNA gene fragments based on differences to their resistance to chemical denaturation, generating a genetic profile of the individual bacterial species within a community (Burr et al, 2006;Zhang et al, 2008). Applying such culture-independent methods to ecotoxicology studies may provide new insights into the pesticide-induced changes that occur to indigenous microbial communities of plants.…”

Section: Introductionmentioning

confidence: 99%

The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere

Zhang

Bai

Hoefel

et al. 2009

Science of The Total Environment

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Although pesticides have been extensively used for controlling insects and disease pathogens of plants, little is known regarding the impacts of applying these pesticides on the microbial community in the plant phyllosphere. Here, we report the effects of cypermethrin pesticide application upon the microbial community of the pepper plant phyllosphere. Assessments were made using culture-independent techniques including phospholipid fatty acid analysis (PLFA) and 16S rRNA gene directed Polymerase Chain Reaction with Denaturing Gradient Gel Electrophoresis (PCR-DGGE). During the 21 day greenhouse study, PLFA results indicated that both total and bacterial biomass increased after application of the pesticide. PLFA profiles also indicated that Gram-negative bacteria became predominant. DGGE analysis confirmed a significant change in bacterial community structure within the phyllosphere following the pesticide application where different dendrogram clusters were observed between control and treated samples. Phylogenetic analysis also suggested a change in bacterial phyla following treatment, where bands sequenced within control cultures were predominantly of the Firmicutes phylum, but those bands sequenced in the treated samples were predominantly members of the Bacteroidetes and γ-Proteobacteria phyla. In conclusion, this study revealed an increase in bacterial abundance and a shift in community composition within the pepper plant phyllosphere following the pesticide application, and highlighted the effective use of PLFA and PCR-DGGE for studying the effect of pesticides upon indigenous phyllosphere microbes. IntroductionThe indigenous microbial communities that reside within the phyllosphere are highly diverse and include many different species of bacteria, filamentous fungi, yeasts and algae (Lindow and Brandl, 2003). The community composition of phyllosphere microbes are affected by many different factors including plant species, fluctuations in ambient temperature, changes to relative humidity, nutrient availability upon the plant surface and direct solar UV radiation (Lindow and Leveau, 2002). Phyllosphere microbes often have a direct positive influence in altering plant surface properties, where

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Assessing the impact of the biological control agentBacillus thuringiensison the indigenous microbial community within the pepper plant phyllosphere

Cited by 50 publications

References 29 publications

The science, development, and commercialization of postharvest biocontrol products

The science, development, and commercialization of postharvest biocontrol products

Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere

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