Assessing the oral microbiota of healthy and alcohol-treated rats using whole-genome DNA probes from human bacteria

Jabbour, Zaher; Nascimento, Cássio do; Kotake, Bruna Gabriela dos Santos; El-Hakim, Michel; Henderson, Janet E.; Albuquerque, Rubens Ferreira de

doi:10.1016/j.archoralbio.2012.07.017

Cited by 13 publications

(13 citation statements)

References 27 publications

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“…In this case, a reduction in species richness was observed along three time-points in alcohol-fed versus pellet-fed animals, and this decrease showed a negative correlation between species richness and the cumulative time mice were alcohol-fed. Another recent work [ 45 ] also found a reduction in species richness, this time in the oral biofilms covering the teeth of rats that received 30-days of a 20% ethanol-containing diet. Besides technical limitations due to the approach used for bacteria detection and quantification in the biofilms of rat teeth (restricted to probes for only 33 bacteria of the human oral microbiome) these authors were able to demonstrate a significant association between alcohol consumption and lower levels of bacterial richness.…”

Section: Resultsmentioning

confidence: 84%

Alcohol and tobacco consumption affects bacterial richness in oral cavity mucosa biofilms

et al. 2014

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BackgroundToday there are more than 2 billion alcohol users and about 1.3 billion tobacco users worldwide. The chronic and heavy use of these two substances is at the heart of numerous diseases and may wreak havoc on the human oral microbiome. This study delves into the changes that alcohol and tobacco may cause on biofilms of the human oral microbiome. To do so, we used swabs to sample the oral biofilm of 22 subjects; including 9 control-individuals with no or very low consumption of alcohol and no consumption of tobacco, 7 who were chronic and heavy users of both substances and 6 active smokers that reported no significant alcohol consumption. DNA was extracted from swabs and the V1 region of the 16S rRNA gene was PCR amplified and sequenced using the Ion Torrent PGM platform, generating 3.7 million high quality reads. DNA sequences were clustered and OTUs were assigned using the ARB SILVA database and Qiime.ResultsWe found no differences in species diversity and evenness among the groups. However, we found a significant decrease in species richness in only smokers and in smokers/drinkers when compared to controls. We found that Neisseria abundance was significantly decreased in both groups when compared to controls. Smokers had significant increases in Prevotella and Capnocytophaga and reductions in Granulicatella, Staphylococcus, Peptostreptococcus and Gemella when compared to the two other groups. Controls showed higher abundance of Aggregibacter, whilst smokers/drinkers had lower abundances of Fusobacteria. Samples from only smokers clustered closer together than to controls and smokers/drinkers, and also had a significant reduction in inter-group dissimilarity distances, indicating a more homogenous group than controls.ConclusionsOur results indicate that the continued use of tobacco or alcohol plus tobacco significantly reduces bacterial richness, which apparently leads to a reduction in inter-group variability, turning the respective biofilms into a more homogenous microenvironment in terms of bacterial community composition, with possible consequences for human oral diseases.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-014-0250-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 84%

Alcohol and tobacco consumption affects bacterial richness in oral cavity mucosa biofilms

et al. 2014

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“…Metabolomics has been used to identify biomarkers of ethanol-induced liver disease [202,203]. Microbiomics may help us understand how ethanol modifies oral bacteria and whether certain bacteria may be more carcinogenic than others [204,205]. We expect that many new discoveries will be made in our understanding of the molecular mechanisms of ethanol-associated OESCC.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms of ethanol-associated oro-esophageal squamous cell carcinoma

et al. 2015

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Alcohol drinking is a major etiological factor of oro-esophageal squamous cell carcinoma (OESCC). Both local and systemic effects of ethanol may promote carcinogenesis, especially among chronic alcoholics. However, molecular mechanisms of ethanol-associated OESCC are still not well understood. In this review, we summarize current understandings and propose three mechanisms of ethanol-associated OESCC: (1) Disturbance of systemic metabolism of nutrients: during ethanol metabolism in the liver, systemic metabolism of retinoids, zinc, iron and methyl groups is altered. These nutrients are known to be associated with the development of OESCC. (2) Disturbance of redox metabolism in squamous epithelial cells: when ethanol is metabolized in oro-esophageal squamous epithelial cells, reactive oxygen species are generated and produce oxidative damage. Meanwhile, ethanol may also disturb fatty-acid metabolism in these cells. (3) Disturbance of signaling pathways in squamous epithelial cells: due to its physico-chemical properties, ethanol changes cell membrane fluidity and shape, and may thus impact multiple signaling pathways. Advanced molecular techniques in genomics, epigenomics, metabolomics and microbiomics will help us elucidate how ethanol promotes OESCC.

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“…Helicobacter pylori is the first conventionally recognised bacterial carcinogen 11 ; another correlation between pathogenic bacteria and oral disease is Porphyromonas gingivalis infection and periodontitis 12 . Moreover, oral microbiota alterations have been detected in alcohol 13 , betel 14 , and cigarette 15 , 16 consumers. Hence, the link between oral cancer-inducing factors and bacterial ecology should not be neglected.…”

Section: Introductionmentioning

confidence: 99%

Bacterial alterations in salivary microbiota and their association in oral cancer

Lee

Chen

Yang

et al. 2017

Sci Rep

157

175

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Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity and the fourth leading malignancy and cause of cancer-related death in the male population of Taiwan. Most cases are detected at advanced stages, resulting in poor prognosis. Therefore, improved detection of early oral health disorders is indispensable. The involvement of oral bacteria in inflammation and their association with OSCC progression provide a feasible target for diagnosis. Due to the nature of oral neoplasms, the diagnosis of epithelial precursor lesions is relatively easy compared with that of other types of cancer. However, the transition from an epithelial precursor lesion to cancer is slow and requires further and continuous follow-up. In this study, we investigated microbiota differences between normal individuals, epithelial precursor lesion patients, and cancer patients with different lifestyle habits, such as betel chewing and smoking, using next-generation sequencing. Overall, the oral microbiome compositions of five genera, Bacillus, Enterococcus, Parvimonas, Peptostreptococcus, and Slackia, revealed significant differences between epithelial precursor lesion and cancer patients and correlated with their classification into two clusters. These composition changes might have the potential to constitute a biomarker to help in monitoring the oral carcinogenesis transition from epithelial precursor lesion to cancer.

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Assessing the oral microbiota of healthy and alcohol-treated rats using whole-genome DNA probes from human bacteria

Abstract: Whole-genome DNA probes prepared from human oral bacteria can cross-react with rats' oral bacterial species. Alcohol consumption is associated with lower levels and diversity of bacterial species in the oral cavity of rats.

Cited by 13 publications

References 27 publications

Alcohol and tobacco consumption affects bacterial richness in oral cavity mucosa biofilms

Alcohol and tobacco consumption affects bacterial richness in oral cavity mucosa biofilms

Molecular mechanisms of ethanol-associated oro-esophageal squamous cell carcinoma

Bacterial alterations in salivary microbiota and their association in oral cancer

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