Assessing the relevance of a multiplexed methodology for proteomic biomarker measurement in the invertebrate species Gammarus fossarum: A physiological and ecotoxicological study

A “Population Dynamics” Perspective on the Delayed Life-History Effects of Environmental Contaminations: An Illustration with a Preliminary Study of Cadmium Transgenerational Effects over Three Generations in the Crustacean Gammarus

“…Methyl farnesoate is a hormone implied in molting, the control of oogenesis, and possibly in testicular development in crustaceans. In support of this hypothesis, we also showed in G. fossarum using a targeted proteomics approach that chronic exposure to 2 µg/L of Cd could inhibit the production of an enzyme specifically involved in the metabolism of this hormone [39]. However, all of these results, which suggest the possibility of an endocrine disruption action of Cd in the amphipods, have only been obtained in sexually mature individuals.…”

“…F0 adult gammarids were exposed during one reproductive cycle to two environmentally relevant concentrations of Cd (0.3 and 3 µg Cd/L). These concentrations were fixed in the range of concentrations used in previous studies [35,[37][38][39] seeking to induce only sublethal effects (reproductive disorders) in F0 spawners. Thereafter, control, 0.3 µg Cd/L, and 3 µg Cd/L parental exposure conditions are indicated by using the abbreviations C-F0, 0.3Cd-F0, and 3Cd-F0, respectively.…”

“…We favored exposure in large volume containers (12 L buckets) to manage such a large number of F0 spawners at the expense of setting up exposure replicates; only replicates of biological measurements were implemented ( Figure S1). We opted for this dose-response design with pseudo-replication at the individual level only, because we had a very good experience of the exposure protocols used, as well as previous knowledge of the expected effects of these cadmium levels in terms of feeding inhibition, disruption of the molting cycle, vitellogenesis, spermatogenesis, and sperm genotoxicity in this species [35,[37][38][39]. However, the production of F1 neonates was lower than expected.…”

In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging

“…G. fossarum organisms were collected from a watercress population and acclimatized to laboratory conditions . Female gammarids at an advanced reproductive stage of vitellogenesis were directly plunge frozen in liquid N 2 and stored at −80°C until cryo‐sectioning .…”

Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host

“…Gammarids were collected from the Bourbre River in France, acclimatized, and maintained in the laboratory, as previously described 32 , fully compliant with all ethical regulations applicable to animal testing and research in France, i.e., ethical approval is not needed for arthropod studies (decret number 2013-118, Art. R. 214-88, 1).…”