Assessing the response of human primary macrophages to defined fibrous architectures fabricated by melt electrowriting

Mondadori, Carlotta; Chandrakar, Amit; Lopa, Silvia; Wieringa, Paul; Talò, Giuseppe; Perego, Silvia; Lombardi, Giovanni; Colombini, Alessandra; Moretti, Matteo; Moroni, Lorenzo

doi:10.1016/j.bioactmat.2022.07.014

Cited by 13 publications

(12 citation statements)

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“…Mondadori et al developed polycaprolactone (PCL) films and 3D scaffolds with varying pore geometries (square, triangle and rhombus grids) to evaluate the ability of these biomaterials to modulate macrophage polarization. 82 The expression of CXCL9 was not found to be associated with the different 3D structures. Among the 3D scaffolds, those with a rhombus grid structure stimulated macrophages to produce the highest levels of CCL5/17/22/24, CCR2/5, CXCL2, IL-1β/1Ra/6/10/13/18, TNF-α and soluble CD163.…”

Section: Effects Of Biomaterials Properties On the Immune Response Of...mentioning

confidence: 90%

Potential effects of biomaterials on macrophage function and their signalling pathways

Zhu,

Wang,

Zhu

et al. 2023

Biomater. Sci.

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Section: Effects Of Biomaterials Properties On the Immune Response Of...mentioning

confidence: 90%

Potential effects of biomaterials on macrophage function and their signalling pathways

Zhu,

Wang,

Zhu

et al. 2023

Biomater. Sci.

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“…In parallel, after 2 days, ASCs were plated on the polycarbonate membrane of trans-wells (Merck, Darmstadt, Germany) at a density of 0.7×10 5 cells/trans-well and left 3 days in appropriate expansion medium. At day 5, macrophages were either maintained in the M0 state in the presence of 20 ng/mL M-CSF or polarized into the M1 phenotype adding to the culture medium IFN-γ (100 ng/ml, Peprotech) and LPS (100 ng/ml, Sigma-Aldrich) ( Mondadori et al, 2023 ). In parallel, at day 5, ASCs were primed with 1 ng/ml of IL-1β.…”

Section: Methodsmentioning

confidence: 99%

Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells

Colombini

Libonati

Cangelosi

et al. 2022

Front. Bioeng. Biotechnol.

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Inflammatory processes contribute to osteoarthritis (OA) severity and progression. Mesenchymal stem cells, particularly those derived from adipose tissue (ASCs), are able to sense and control the inflammatory environment. This immunomodulatory potential can be boosted by different priming strategies based on inflammatory stimulation. The aim of the present study is to investigate the transcriptional modulation of a huge panel of genes and functionally verify the predicted immunomodulatory ability of ASCs after interleukin one beta (IL-1β) priming. ASCs were isolated from adipose tissue obtained from three donors and expanded. After stimulation with 1 ng/ml of IL-1β for 48 h, cells were collected for gene array and functional tests. Pooled cells from three donors were used for RNA extraction and gene array analysis. Gene Ontology (GO) enrichment analysis and Gene Set Enrichment Analysis (GSEA) were performed to assess the involvement of the modulated genes after priming in specific biological processes and pathways. Functional co-culture tests of ASCs with T cells and macrophages were performed to assess the ability of primed ASCs to modulate immune cell phenotype. Among the overall genes analyzed in the gene array, about the 18% were up- or down-regulated in ASCs after IL-1β priming. GO enrichment analysis of up- or down-regulated genes in ASCs after IL-1β priming allowed identifying specific pathways involved in the modulation of inflammation and extracellular matrix remodeling. The main processes enriched according to the GSEA are related to the inflammatory response and cell proliferative processes. Functional tests on immune cells showed that primed and non-primed ASCs induced a decrease in the CD3+ T lymphocytes survival rate and an anti-inflammatory macrophage polarization. In conclusion, IL-1β priming represents a tailored strategy to enhance the ability of ASCs to direct macrophages towards an anti-inflammatory phenotype and, consequently, improve the efficacy of ASCs in counteracting the OA inflammatory component.

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“…Indeed, cells tend to behave similar to those cultured in 2D conditions since fibers are too large for them to recognize the differences in fiber deposition. 43 3.1.3. Macrofiber Scaffolds Promoted More New Bone Formation In Vivo.…”

Section: Macrofiber Scaffolds With Different Fiber Arrangements Showe...mentioning

confidence: 99%

Macro- or Microfiber Scaffolds with Different Fiber Arrangements: Determining the Best Design for Osteogenesis

Yang,

Cai,

et al. 2024

Ind. Eng. Chem. Res.

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Bone tissue engineering scaffolds with varied 3D structures have been investigated extensively. The arrangements and diameters of scaffold fibers are usually considered during the scaffold manufacturing processes. There is still controversy over the optimal angle of arrangement and fiber diameter of scaffolds for bone tissue engineering. To highlight the problem, we herein designed macrofiber (diameter: 551.3 ± 22.11 μm) and microfiber (diameter: 10.8 ± 0.85 μm) scaffolds with fibers arranged at 90, 60, 30, and 15°. Physicochemical characterization revealed that macrofiber scaffolds had better mechanical properties and hydrophilicity than microfiber ones. Although the different fiber arrangements of the macrofiber scaffold showed no significant differences in alkaline phosphatase expression and calcium nodule deposition, the macrofiber scaffold enhanced new bone formation obviously in the skull defect model. The microfiber scaffolds with deposition angles of 90 and 60° efficiently promoted cell osteogenic differentiation by regulating the YAP pathway and enhancing the expression of Runx2 and OCN. However, microfiber scaffolds seemed to be a barrier to new bone formation when applied for bone tissue repair of skull defects. Poor mechanical properties severely limited the biological application of microfiber scaffolds. The macrostructures guaranteed sufficient mechanical strength for osteogenic space maintenance. For clinical application, macrofiber scaffolds with high mechanical strength might be a promising therapy for repairing large bone tissue defects.

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Assessing the response of human primary macrophages to defined fibrous architectures fabricated by melt electrowriting

Cited by 13 publications

References 65 publications

Potential effects of biomaterials on macrophage function and their signalling pathways

Potential effects of biomaterials on macrophage function and their signalling pathways

Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells

Macro- or Microfiber Scaffolds with Different Fiber Arrangements: Determining the Best Design for Osteogenesis

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