The ability of a 2-stage (filtration + UV/Ag-TiO 2 /O 3) ballast water treatment process to control non-indigenous species introduction has been assessed. The removal efficiencies of turbidity and phytoplankton were monitored during the filtration pretreatment phase, and the degradation of phytoplankton (Dunaliella salina and Phacodactylum tricornutum) chlorophyll a (chl-a) by UV/Ag-TiO 2 /O 3 were investigated. Results showed that 50 μm screen filtration could remove a small part of phytoplankton, and reduce the turbidity. Compared to individual unit processes with ozone or UV/Ag-TiO 2 , the removal of chl-a by the combined UV/Ag-TiO 2 /O 3 process was enhanced, and the effluent exhibited a persistence toxicity within 24 h. The results indicate that a combination of filtration and UV/Ag-TiO 2 /O 3 advanced oxidation maybe a promising method for ballast water treatment.