Assessing the stability of polyphenol content in red rooibos herbal tea using traditional methods and high-resolution mass spectrometry: Implications for studying dietary interventions in preclinical rodent studies

Mahamoud, Reem; Bowman, David T.; Ward, Wendy E.; Mangal, Vaughn

doi:10.1016/j.foodchem.2024.139068

Food Chemistry

2024

DOI: 10.1016/j.foodchem.2024.139068

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Assessing the stability of polyphenol content in red rooibos herbal tea using traditional methods and high-resolution mass spectrometry: Implications for studying dietary interventions in preclinical rodent studies

Reem Mahamoud,

David T. Bowman,

Wendy E. Ward

et al.

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2024

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Cited by 2 publications

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In vitro Antioxidant and Antitumor Activity of Rooibos (Aspalathus linearis) Extract on Colon, Prostate, and Liver Cancer Cells

Malibary

2024

Curr Res Nutr Food Sci

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Rooibos tea (Aspalathus linearis) is recognized for its antioxidant properties, due to distinctive compounds such as aspalathin. This study examined the antioxidant potential of rooibos extract on three cancer cell lines (PC-3, HCT-116, and HepG2) to assess its overall antioxidant potential. Additionally, the inhibitory effect of aqueous rooibos extract at a concentration of 1,000 µg/ml was assessed on the three cancer cell lines. The cytotoxic effects of aqueous rooibos extract and cisplatin were compared for the cancer cell lines, with HepG2 cells showing the highest sensitivity to rooibos extract (IC50 = 1,399.41 ± 62.73 µg/ml) and PC-3 cells displaying the highest resistance (IC50 = 2,431.51 ± 98.71 µg/ml). Cisplatin consistently exhibited lower IC50 values across all cell lines, indicating a stronger inhibitory effect on cancer cell proliferation, notably for HCT-116 (IC50 = 2.53 ± 0.21 µg/ml) and HepG2 cells (IC50 = 3.67 ± 0.25 µg/ml). However, the total antioxidant capacity of rooibos extract (68.91 ± 4.07 mg GAE/g) is comparable to that of the ascorbic acid standard (74.19 ± 3.97 mg GAE/g), showing no statistically significant difference. Moreover, rooibos extract is a potent antioxidant, with lower IC50 values in the DPPH (3.62 ± 0.10 µg/ml), ABTS (2.13 ± 0.13 µg/ml), and H2O2 assays (4.97 ± 0.01 µg/ml) compared to ascorbic acid and EDTA. However, in the metal chelating activity assay, rooibos extract showed a similar performance (IC50 20.89 ± 0.09 µg/ml) to EDTA (IC50 21.16 ± 1.67 µg/ml), suggesting comparable efficacy in this measure. These results demonstrate the strong antioxidant and anticancer potential of rooibos extract, suggesting it as a promising complement in cancer treatment.

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In vitro Antioxidant and Antitumor Activity of Rooibos (Aspalathus linearis) Extract on Colon, Prostate, and Liver Cancer Cells

Malibary

2024

Curr Res Nutr Food Sci

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Characteristic Polyphenols in 15 Varieties of Chinese Jujubes Based on Metabolomics

Shao,

Li,

Chen

et al. 2024

Metabolites

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Background: Jujube is a homologous herb of medicine and food, and polyphenols are key in determining the functional effects of jujubes. Methods: In this study, characteristic polyphenols in 15 varieties of Chinese jujubes were investigated based on untargeted metabolomics. Results: The results showed that a total of 79 characteristic polyphenols were identified in the 15 varieties of Chinese jujube, and 55 characteristic polyphenols such as syringetin, spinosin and kaempferol were reported for the first time. Scopoletin (63.94% in LZYZ), pectolinarin (22.63% in HZ) and taxifolin (19.69% in HZ) contributed greatly and presented significant (p < 0.05) differences in the 15 varieties of Chinese jujubes. HZ was characterized by pectolinarin, erianin and wogonoside, while XSHZ, NYDZ and RQHZ, with similar polyphenol profiles, were characterized by (+)-catechin, combretastatin A4 and tectorigenin. JSBZ, HMDZ, TZ, JCJZ and HPZ had similar polyphenol profiles of galangin, isoferulic acid and hydroxysafflor yellow A. Conclusions: Metabolomics is critical in grasping the full polyphenol contents of jujubes, and the differences in the polyphenol profiles and characteristic individual polyphenols of the 15 varieties of Chinese jujubes were well analyzed by principal component analysis (PCA).

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Assessing the stability of polyphenol content in red rooibos herbal tea using traditional methods and high-resolution mass spectrometry: Implications for studying dietary interventions in preclinical rodent studies

Cited by 2 publications

References 57 publications

In vitro Antioxidant and Antitumor Activity of Rooibos (Aspalathus linearis) Extract on Colon, Prostate, and Liver Cancer Cells

In vitro Antioxidant and Antitumor Activity of Rooibos (Aspalathus linearis) Extract on Colon, Prostate, and Liver Cancer Cells

Characteristic Polyphenols in 15 Varieties of Chinese Jujubes Based on Metabolomics

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