Assessing the suitability of capillary electrophoresis‐mass spectrometry for biomarker discovery in plasma‐based metabolomics

Abstract

The actual utility of capillary electrophoresis‐mass spectrometry (CE‐MS) for biomarker discovery using metabolomics still needs to be assessed. Therefore, a simulated comparative metabolic profiling study for biomarker discovery by CE‐MS was performed, using pooled human plasma samples with spiked biomarkers. Two studies have been carried out in this work. Focus of study I was on comparing two sets of plasma samples, in which one set (class I) was spiked with five isotope‐labeled compounds, whereas another set (class II) was spiked with six different isotope‐labeled compounds. In study II, focus was also on comparing two sets of plasma samples, however, the isotope‐labeled compounds were spiked to both class I and class II samples but with concentrations which differ by a factor two between both classes (with one compound absent in each class). The aim was to determine whether CEMS‐based metabolomics could reveal the spiked biomarkers as the main classifiers, applying two different data analysis software tools (MetaboAnalyst and Matlab). Unsupervised analysis of the recorded metabolic profiles revealed a clear distinction between class I and class II plasma samples in both studies. This classification was mainly attributed to the spiked isotope‐labeled compounds, thereby emphasizing the utility of CE‐MS for biomarker discovery.

The Nanomaterial Metabolite Corona Determined Using a Quantitative Metabolomics Approach: A Pilot Study

“…The employed method was adapted from the following works. [ 57,58 ] In short, 10% acetic acid (pH 2.2) was used as the BGE and separation was conducted on a bare fused silica capillary (70 cm in length and 50 µm i.d.). Samples were injected hydrodynamically at 50 mbar for 20 s, corresponding to ≈1.2% (≈17 nL) of the total capillary volume.…”

Metabolomics meets lipidomics: Assessing the small molecule component of metabolism

“…Major improvements in TA B L E 1 Technological platforms used to resolve polar and lipid metabolite diversity. The most common and successful applications are described [31][32][33][34][35][36] Flow [37] Complex lipids: Glycerolipids, glycerophospholipids, sphingolipids [38][39][40] Surface [41][42][43] Fatty acids Complex lipids: Glycerolipids, glycerophospholipids, sphingolipids [44][45][46][47][48] Gas [49][50][51] Fatty acids, oxysterols, oxylipins, steroids, aldehydes [24,[52][53][54][55][56] Liquid [57][58][59][60][61] Fatty acids, oxysterols, oxylipins, steroids, acyl carnitines [56,[62][63][64] Complex lipids: Glycerolipids, glycerophospholipids, sphingolipids [65,66] Capillary electrophoresis mass spectrometry (CE-MS) [67][68][69][70]…”

Capillary electrophoretic profiling of in‐bone tryptic digests of proteins as a potential tool for the detection of inflammatory states in oral surgery

“…CE is a powerful separation method with wide applicability in the analysis of proteins [12,13] and peptides [14,15], including their complex mixtures in proteomic, peptidomic, and metabolomic studies [16][17][18][19][20]. CE-UV and CE-MS profiling of complex protein mixtures, such as proteins in biological fluids, various human, animal, and plant tissues, and food extracts, enables differentiation of the origin and character of these samples, e.g.…”