Assessing the Taxonomic Validity of Austrodiplostomum spp. (Digenea: Diplostomidae) through Nuclear and Mitochondrial Data

Sereno-Uribe, Ana L.; Andrade-Gómez, Leopoldo; Núñez, Margarita Ostrowski de; León, Gerardo Pérez‐Ponce de; García-Varela, Martìn

doi:10.1645/18-51

Cited by 15 publications

(9 citation statements)

References 27 publications

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“…The value of concatenating the nuclear rRNA genes with the mitochondrial rRNA genes can potentially increase species-level taxonomic resolution. The use of genetic markers from two different loci has been suggested and utilized by various studies, but they have rarely been combined to improve phylogenetic resolution for molecular systematics [ 15 , 31 , 52 , 57 – 59 ]. Our findings, therefore, demonstrate the potential of combining both the nuclear and mitochondrial DNA as an alternative genetic marker for trematode molecular systematics.…”

Section: Discussionmentioning

confidence: 99%

The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes

Chan

Saralamba

et al. 2022

BMC Genomics

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Background Genetic markers like the nuclear ribosomal RNA (rRNA) genes, internal transcribed spacer regions, mitochondrial protein-coding genes, and genomes have been utilized for molecular identification of parasitic trematodes. However, challenges such as the design of broadly applicable primers for the vast number of species within Digenea and the genetic markers’ ability to provide sufficient species-level resolution limited their utility. This study presented novel and broadly applicable primers using the mitochondrial 12S and 16S rRNA genes for Digenea and aimed to show their suitability as alternative genetic markers for molecular identification of orders Plagiorchiida, Echinostomida, and Strigeida. Results Our results revealed that the mitochondrial 12S and 16S rRNA genes are suitable for trematode molecular identification, with sufficient resolution to discriminate closely related species and achieve accurate species identification through phylogenetic placements. Moreover, the robustness of our newly designed primers to amplify medically important parasitic trematodes encompassing three orders was demonstrated through successful amplification. The convenience and applicability of the newly designed primers and adequate genetic variation of the mitochondrial rRNA genes can be useful as complementary markers for trematode molecular-based studies. Conclusions We demonstrated that the mitochondrial rRNA genes could be alternative genetic markers robust for trematode molecular identification and potentially helpful for DNA barcoding where our primers can be widely applied across the major Digenea orders. Furthermore, the potential of the mitochondrial rRNA genes for molecular systematics can be explored, enhancing their appeal for trematode molecular-based studies. The novelty of utilizing the mitochondrial rRNA genes and the designed primers in this study can potentially open avenues for species identification, discovery, and systematics in the future.

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Section: Discussionmentioning

confidence: 99%

The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes

Chan

Saralamba

et al. 2022

BMC Genomics

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“…Our results, along with other recent molecular phylogenetic studies (e.g. Blasco-Costa and Locke, 2017; Hernández-Mena et al ., 2017; Locke et al ., 2018; Sereno-Uribe et al ., 2019; Achatz et al ., 2020, 2021 a , 2021 b , 2021 c , 2022, in press; Queiroz et al ., 2020; Locke et al ., 2021), strongly suggest that the most recently accepted subfamilies of the Diplostomidae cannot be considered valid. Achatz et al .…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogenetic analysis of Neodiplostomum and Fibricola (Digenea, Diplostomidae) does not support host-based systematics

et al. 2022

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Fibricola and Neodiplostomum are diplostomid genera with very similar morphology that are currently separated based on their definitive hosts. Fibricola spp. are normally found in mammals, while Neodiplostomum spp. typically parasitize birds. Previously, no DNA sequence data was available for any member of Fibricola. We generated nuclear ribosomal and mtDNA sequences of Fibricola cratera (type-species), Fibricola lucidum and 6 species of Neodiplostomum. DNA sequences were used to examine phylogenetic interrelationships among Fibricola and Neodiplostomum and re-evaluate their systematics. Molecular phylogenies and morphological study suggest that Fibricola should be considered a junior synonym of Neodiplostomum. Therefore, we synonymize the two genera and transfer all members of Fibricola into Neodiplostomum. Specimens morphologically identified as Neodiplostomum cratera belonged to 3 distinct phylogenetic clades based on mitochondrial data. One of those clades also included sequences of specimens identified morphologically as Neodiplostomum lucidum. Further study is necessary to resolve the situation regarding the morphology of N. cratera. Our results demonstrated that some DNA sequences of N. americanum available in GenBank originate from misidentified Neodiplostomum banghami. Molecular phylogentic data revealed at least 2 independent host-switching events between avian and mammalian hosts in the evolutionary history of Neodiplostomum; however, the directionality of these host-switching events remains unclear.

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“…COI sequences are required to test this hypothesis, and to determine if the geographic pattern mirrors that of other trematodes parasitizing migratory fish-eating birds (e.g. Briosio-Aguilar et al ., 2018; Sereno-Uribe et al ., 2019 a ).…”

Section: Discussionmentioning

confidence: 99%

“…However, we were unsuccessful in achieving the amplification, which is noteworthy because the same set of primers and protocols were followed by us when studying other diplostomids (e.g. García-Varela et al ., 2016; Sereno-Uribe et al ., 2019 a , 2019 b ). To report the genetic variation of a mitochondrial gene among lineages uncovered by ITS, albeit not useful for comparison with other Posthodiplostomum isolates, a subsample was sequenced using the forward primer JB3, 5′-TTTTTTGGGCATCCTGAGGTTTAT-3′ and the reverse primer JB4, 5′-TAAAGAACATAATGAAATTG-3′ (Bowles and McManus, 1993).…”

Section: Methodsmentioning

confidence: 99%

Assessing the genetic diversity of the metacercariae of Posthodiplostomum minimum (Trematoda: Diplostomidae) in Middle American freshwater fishes: one species or more?

et al. 2021

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Trematode taxonomy is mainly based on the morphological traits of adults. The identification of metacercariae is challenging because such traits are not developed in larval forms, and they even may show some level of morphological variability. Studies testing the potential correspondence between morphological differences and genetic variation of parasites are still lacking. The metacercariae of Posthodiplostomum minimum are probably the diplostomids more widely distributed in North and Middle American freshwater fish, and their intraspecific morphological variability has been attributed to the effect exerted by the host. Here, we tested the hypothesis whether they represent a single species, or a species complex by assessing the genetic divergence and phylogenetic relationships of metacercariae sampled from several host species in a wide geographical range across Middle America. The internal transcribed spacers (ITS1-5.8S-ITS2), and the mitochondrial COI gene were sequenced for 124 and 55 metacercariae, respectively. Phylogenetic analysis inferred from ITS sequences uncovered six well-supported monophyletic lineages. The six lineages show no correspondence to any Posthodiplostomum species for which sequences are available thus far in GenBank. Lineages exhibit some degree of host specificity; Lineages I, II, IV and V are primarily parasites of cyprinodontiforms of the families Poeciliidae, Goodeidae, Profundulidae and Fundulidae. In poeciliids there are at least four candidate species of Posthodiplostomum, some of them occurring in sympatry; instead, Lineages II and VI are exclusively parasites of cichlids. This study contributes to our understanding of the diversity of larval forms of diplostomids and provides an opportunity to further study their life cycles.

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Assessing the Taxonomic Validity of Austrodiplostomum spp. (Digenea: Diplostomidae) through Nuclear and Mitochondrial Data

Cited by 15 publications

References 27 publications

The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes

The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes

Molecular phylogenetic analysis of Neodiplostomum and Fibricola (Digenea, Diplostomidae) does not support host-based systematics

Assessing the genetic diversity of the metacercariae of Posthodiplostomum minimum (Trematoda: Diplostomidae) in Middle American freshwater fishes: one species or more?

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