Assessment and comparison of probiotic potential of four <i>Lactobacillus</i> species isolated from feces samples of Iranian infants

Halimi, Shahnaz; Mirsalehian, Akbar

doi:10.1111/1348-0421.12352

Cited by 21 publications

(7 citation statements)

References 28 publications

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“…This study reveals that most isolates are more resistant to acidic condition than the control strain, which agrees with the previous report from Malaysia ( Shokryazdan et al, 2014 ). In addition, the results show that these 18 strains have stronger bacteriostatic effect on Staphylococcus aureus than Escherichia coli which is in agreement with previous reports ( Shokryazdan et al, 2014 ; Halimi and Mirsalehian, 2016 ). Antimicrobial activity is another criterion for selecting probiotic bacteria.…”

Section: Discussionsupporting

confidence: 93%

Novel exopolysaccharide derived from probiotic Lactobacillus pantheris TCP102 strain with immune-enhancing and anticancer activities

Sheng

Pan

et al. 2022

Front. Microbiol.

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Probiotics are gaining attention due to their functions of regulating the intestinal barrier and promoting human health. The production of exopolysaccharide (EPS) is one of the important factors for probiotics to exert beneficial properties. This study aimed to screen exopolysaccharides-producing lactic acid bacteria (LAB) and evaluate the probiotic potential. we obtained three exopolysaccharide fractions (EPS1, EPS2, and EPS3) from Lactobacillus pantheris TCP102 and purified by a combination of ion-exchange chromatography and gel permeation chromatography. The structures of the fractions were characterized by FT-IR, UV, HPLC, and scanning electron microscopy (SEM) analysis. The Mw of EPS1, EPS2, and EPS3 were approximately 20.3, 23.0, and 19.3 kDa, and were mainly composed of galactose, glucose, and mannose, with approximate molar ratios of 2.86:1:1.48, 1.26:1:1, 1.58:1.80:1, respectively. Furthermore, SEM analysis demonstrated that the three polysaccharide fractions differ in microstructure and surface morphology. Additionally, preliminary results for immune-enhancing and anticancer activities reveal that these EPSs significantly induced the production of nitric oxide (NO), TNF-α, and IL-6 in Ana-1 cells and peritoneal macrophage cells. Meanwhile, the EPSs also significantly suppressed the proliferation of HCT-116, BCG-803, and particularly A-2780 cells. The results suggest that the three novel EPSs isolated from Lactobacillus pantheris TCP102 can be regarded as potential application value in functional food and natural antitumor drugs.

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Section: Discussionsupporting

confidence: 93%

Novel exopolysaccharide derived from probiotic Lactobacillus pantheris TCP102 strain with immune-enhancing and anticancer activities

Sheng

Pan

et al. 2022

Front. Microbiol.

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“…Another study reported that Limosilactobacillus fermentum CRRL1446, with feruloyl esterase activity, showed resistance to 0.3% oxgall ( w/v ), and its enzymatic activity was increased upon exposure to bile 24 . Halimi and Mirsalehian 25 reported that all 15 Lactobacillus gasseri strains isolated from the feces of infants were highly sensitive to bile salts, with survival rates within 10%. Therefore, the good viability of the 11 Lactobacillus strains in this study indicates that they could survive under host gastrointestinal conditions.…”

Section: Resultsmentioning

confidence: 99%

Broad‐spectrum antimicrobial activity of cinnamoyl esterase‐producing Lactobacilli and their application in fermented rice bran

et al. 2021

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BACKGROUND Cinnamoyl esterase (CE) can release antioxidant phenolic acids from its non‐digestible ester‐linked form. Fermentation using CE‐producing lactic acid bacteria (LAB) can be useful in the food industry because of its ability to produce bioactive compounds and antibacterial metabolites. The purpose of this study was to confirm the food applicability of LAB with CE‐producing ability and broad‐spectrum antibacterial activity. RESULTS Among the 219 bacterial strains identified in infant feces, five Lactobacillus gasseri and six Limosilactobacillus fermentum with a high CE activity were isolated. The survival rate of all selected LABs was > 95% at pH 2.5 for 3 h and > 70% when treated with 0.3% bile salt for 4 h. Moreover, cell‐free supernatants of all strains strongly inhibited five food‐borne bacterial pathogens (Listeria monocytogenes, Salmonella enterica, Escherichia coli O157:H7, Bacillus cereus, and Staphylococcus aureus) and three toxin‐producing fungal pathogens (Aspergillus niger, Penicillium sp., and Fusarium oxysporum). To improve phenolic acid content and rice bran preservation, Limosilactobacillus fermentum J2 with the strongest CE activity and Lactobacillus gasseri N2 with the strongest antibacterial activity were used in rice bran fermentation, respectively. FRB‐J2 (fermented rice bran with Limosilactobacillus fermentum J2) and FRB‐N2 (fermented rice bran with Lactobacillus gasseri N2) significantly increased caffeic acid and ferulic acid (P < 0.01). FRB‐J2 and FRB‐N2 artificially inoculated with F. oxysporum showed no visible fungal growth during the test period (21 days). CONCLUSION Fermentation by Limosilactobacillus fermentum J2 and Lactobacillus gasseri N2 can help extend the shelf life of rice bran‐based products and produce bioactive compounds. © 2021 Society of Chemical Industry.

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“…Species-speci c primer sets [Integrated DNA Technologies (IDT), Science Park RD, Singapore] of Lpla-3 (5'-ATTCATAGTCTAGTTGGAGGT-3') and Lpla-2 (5'-CCTGAACTGAGAGAATTTBA-3'), with their respective sequence originated from Halimi and Mirsalehian [20], were used to quantify changes of L. plantarum gene transcript level and their proportion within gut ingesta from different sections (i.e. stomach, ileum, caecum and colon) at 0, 1, 4, 8 and 16 h after administration of microencapsulated L. plantarum LAB12.…”

Section: Primersmentioning

confidence: 99%

Chemoprevention by Microencapsulated Lactobacillus plantarum LAB12 against Orthotopic Colorectal Cancer Mice is Associated with Apoptosis and Anti-angiogenesis

Fareez

Lim

Ramasamy

2022

Preprint

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The prevalence of colorectal cancer (CRC) has been widely linked to gut dysbiosis, a compositional imbalance of gut microbiota due to unhealthy lifestyles and dietary habits. Consumption of microencapsulated probiotic may potentially restore the compositional balance of gut microbiota in favour of prevention against CRC. In this regard, the present study was undertaken to determine the fate of microencapsulated Lactobacillus plantarum LAB12 in the gastrointestinal tract (GIT) and thereafter evaluate the chemopreventive effect of microencapsulated L. plantarum LAB12 in vivo. The targeted release of L. plantarum LAB12 from Alg-based microcapsules at different gut sections were examined by means of confocal microscopy and qPCR. Microcapsules loaded with L. plantarum LAB12 remained intact in the stomach. Free L. plantarum LAB12 were present in abundance (> 7 log CFU) only in the intestines. Subsequently, the chemopreventive properties of microencapsulated L. plantarum LAB12 were validated against NU/NU nude mice bearing orthotopic transplanted CT-26 CRC. Orthotopic mice pre-supplemented with microencapsulated L. plantarum LAB12 were presented with significantly (p < 0.05) reduced tumour volume (-98.87%) and weight (-89.27%) when compared to control. The chemopreventive effect could be attributed to apoptosis and anti-angiogenesis mediated, at least in part, through upregulation of p53 (+ 45.4%) and caspase-3 (+ 82.4%), and downregulation of COX-2 (-57.9%), VEGF (-66.8%) and PECAM-1 (-64.1%). Altogether, this study strongly implied the possibility of having L. plantarum LAB12-loaded microcapsules safely incorporated into a wide variety of food and nutraceutical products for prevention against CRC.

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Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Cited by 21 publications

References 28 publications

Novel exopolysaccharide derived from probiotic Lactobacillus pantheris TCP102 strain with immune-enhancing and anticancer activities

Novel exopolysaccharide derived from probiotic Lactobacillus pantheris TCP102 strain with immune-enhancing and anticancer activities

Broad‐spectrum antimicrobial activity of cinnamoyl esterase‐producing Lactobacilli and their application in fermented rice bran

Chemoprevention by Microencapsulated Lactobacillus plantarum LAB12 against Orthotopic Colorectal Cancer Mice is Associated with Apoptosis and Anti-angiogenesis

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