Assessment and Detection of Gene Flow

Wei, Wei; Kwit, Charles; Millwood, Reginald J.; Moon, Hong S.; Stewart, C. Neal

doi:10.1002/9781118352670.ch2

Cited by 3 publications

(4 citation statements)

References 97 publications

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“…rapid growth and early flowering) may enhance hybrids fitness ( Mercer et al . 2007 ; Wei et al . 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Performance of hybrid progeny formed between genetically modified herbicide-tolerant soybean and its wild ancestor

Guan¹,

Peng-fei²,

Wei³

et al. 2015

AoB PLANTS

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Gene flow may occur between genetically modified (GM) crops and wild relatives and the fate of escaped transgenes depends on the performance of hybrids and the fitness of the transgene. Hybrids were formed by hand-crossing a GM strain of glyphosate-tolerant soybean and its non-GM counterpart with wild soybean and were assessed in this study. The hundred-seed weight of hybrids was significantly higher than that of wild soybean. However, no overall difference in plant growth was found between GM and non-GM hybrids. The results suggest that the herbicide-resistant transgene may not pose fitness costs and could persist in nature.

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“…rapid growth and early flowering) may enhance hybrids fitness ( Mercer et al . 2007 ; Wei et al . 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Performance of hybrid progeny formed between genetically modified herbicide-tolerant soybean and its wild ancestor

Guan¹,

Peng-fei²,

Wei³

et al. 2015

AoB PLANTS

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“…Eventually, Speed OPIOM could be exploited for assessing gene flow from RSFP-labeled transgenic crops to wild relatives and for ecological monitoring of transgenic plants in the environment, which is an ever important factor in biotechnology risk assessment. 49 4. METHODS 4.1.…”

Section: Discussionmentioning

confidence: 99%

“…Speed OPIOM could therefore be used to monitor gene expression associated with pollutants (soil or air), the presence of pathogens, water, or nitrogen content. Eventually, Speed OPIOM could be exploited for assessing gene flow from RSFP-labeled transgenic crops to wild relatives and for ecological monitoring of transgenic plants in the environment, which is an ever important factor in biotechnology risk assessment …”

Section: Concluding Remarksmentioning

confidence: 99%

Dynamic Contrast for Plant Phenotyping

et al. 2020

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Noninvasiveness, minimal handling, and immediate response are favorable features of fluorescence readout for high-throughput phenotyping of labeled plants.Yet, remote fluorescence imaging may suffer from an autofluorescent background and artificial or natural ambient light. In this work, the latter limitations are overcome by adopting reversibly photoswitchable fluorescent proteins (RSFPs) as labels and Speed OPIOM (out-of-phase imaging after optical modulation), a fluorescence imaging protocol exploiting dynamic contrast. Speed OPIOM can efficiently distinguish the RSFP signal from autofluorescence and other spectrally interfering fluorescent reporters like GFP. It can quantitatively assess gene expressions, even when they are weak. It is as quantitative, sensitive, and robust in dark and bright light conditions. Eventually, it can be used to nondestructively record abiotic stress responses like water or iron limitations in real time at the level of individual plants and even of specific organs. Such Speed OPIOM validation could find numerous applications to identify plant lines in selection programs, design plants as environmental sensors, or ecologically monitor transgenic plants in the environment.

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“…This method is quicker and less laborious for determining pollen flow than analyzing progeny from recipient plants (e.g. antibiotic screening, PCR, FP screening) [ 8 ]. Drawing upon this previous body of work, it is logical to conceptualize a method to determine bioconfinement efficacy using FP tagging utilizing an improved fluorescent protein for plants.…”

Section: Introductionmentioning

confidence: 99%

An orange fluorescent protein tagging system for real-time pollen tracking

et al. 2013

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BackgroundMonitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification.ResultsWe report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged Nicotiana hybrid that could be used for the incidence of gene flow. Nicotiana tabacum ‘TN 90’ and Nicotiana glauca were successfully transformed via Agrobacterium tumefaciens to express the orange fluorescent protein gene, tdTomato-ER, in pollen and a green fluorescent protein gene, mgfp5-er, was expressed in vegetative structures of the plant. Hybrids were created that utilized the fluorescent proteins as a research tool for monitoring pollen movement and gene flow. Manual greenhouse crosses were used to assess hybrid sexual compatibility with N. tabacum, resulting in seed formation from hybrid pollination in 2% of crosses, which yielded non-viable seed. Pollen transfer to the hybrid formed seed in 19% of crosses and 10 out of 12 viable progeny showed GFP expression.ConclusionThe orange fluorescent protein is visible when expressed in the pollen of N. glauca, N. tabacum, and the Nicotiana hybrid, although hybrid pollen did not appear as bright as the parent lines. The hybrid plants, which show limited ability to outcross, could provide bioconfinement with the benefit of detectable pollen using this system. Fluorescent protein-tagging could be a valuable tool for breeding and in vivo ecological monitoring.

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Assessment and Detection of Gene Flow

Cited by 3 publications

References 97 publications

Performance of hybrid progeny formed between genetically modified herbicide-tolerant soybean and its wild ancestor

Performance of hybrid progeny formed between genetically modified herbicide-tolerant soybean and its wild ancestor

Dynamic Contrast for Plant Phenotyping

An orange fluorescent protein tagging system for real-time pollen tracking

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