Assessment and modifications of digestion procedures to determine trace elements in urine of hypertensive and diabetes mellitus patients

Momen, Awad Abdalla; Khalid, Mohammed Awad Ali; Elsheikh, Malik Abdalla Abdelrahman; Ali, Dafaalla Mohamed Hag

doi:10.4103/1658-600x.120847

Cited by 7 publications

(2 citation statements)

References 29 publications

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“…The crucibles were covered and kept at room temperature (~ 25°C) for three minutes (pre-digestion time), as illustrated in Figure (2). Subsequently, Te on crucibles were introduced into a microwave oven and subjected to a one-stage digestion program at 600 W [19]. After completing the digestion process, all samples were taken out of the microwave and let to cool down to the ambient temperature (~ 0.5 mL).…”

Section: Sample Digestionmentioning

confidence: 99%

RETRACTED: Occupational exposure to heavy metal dust and its hazardous effects on non-ferrous foundry workers' health

Fahim,

Hasani,

El-Khawaga

et al. 2024

Preprint

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Exposure to metal dust is a significant occupational hazard for foundry workers, which may result in DNA damage, lipid peroxidation, and organ toxicity. This study aimed to investigate exposure to potentially toxic metals and oxidative stress indices and assess the health risk of occupational exposure to metal dust among foundry workers. This study utilized a case-control study with 60 male exposed workers and 30 male controls. Environmental and biological exposures to a cocktail of metals were examined by measuring the concentration of Aluminum (Al), Zinc (Zn), Copper (Cu), Cadmium (Cd), Nickel (Ni), and Chromium (Cr) in the air of the workplace, as well as in the blood of the exposed workers. Malondialdehyde (MDA), reduced blood glutathione (GSH) and urinary 8- hydroxydeoxy guanosine (8-OH-dG) were measured as biomarkers of oxidative stress. All air measurements were below the maximum allowable limits (MAL except for Al and Ni, which were above MAL according to ACGIH and NIOSH. Here are significantly elevated Blood Al, Zn, Cu, and Pb levels in exposed workers. Moreover, MDA and 8-OHdG levels significantly increased (P < 0.0001). In contrast, the mean level of GSH was reduced considerably in exposed workers compared to the control group (P < 0.0001). The MDA acts as a marker with the highest Area Under the Curve (AUC), enabling effective differentiation between the exposed and control subjects (AUC = 0.968; Sensitivity = 90%, Specificity = 100%; P < 0.0001), MDA may be used for follow up in exposed workers as an oxidative stress marker. Our study shows a significant increase in liver and kidney parameters as ALT AST, ALP, γGT, LDH, Urea and Uric acid with (P < 0.05). At the same time, there was a significant decrease in Hemoglobin in exposed workers (P < 0.05). Workers occupationally exposed to these metals for prolonged periods possessed higher metal levels in their bodies, which is associated with increased oxidative stress, which consequently causes DNA damage.

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Section: Sample Digestionmentioning

confidence: 99%

RETRACTED: Occupational exposure to heavy metal dust and its hazardous effects on non-ferrous foundry workers' health

Fahim,

Hasani,

El-Khawaga

et al. 2024

Preprint

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“…The crystallinity of DNPs was confirmed using electron diffraction microscopy. The chemical compound contents were scanned by gas chromatography coupled with a mass spectrometer (GC/MS) 25 . The elemental analysis was performed by an inductively coupled plasma optical emission spectrometer (ICP‐OES) (720‐Agilent Technologies) 26 …”

Section: Methodsmentioning

confidence: 99%

Linseed ameliorates renal apoptosis in rat fetuses induced by single or combined exposure to diesel nanoparticles or fenitrothion by inhibiting transcriptional activation of p21/p53 and caspase‐3/9 through pro‐oxidant stimulus

Ibrahim

Abdelgaid

El-Desouky

et al. 2021

Environmental Toxicology

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Gestational exposure to environmental pollutants can induce oxidative injury and apoptosis since the fetal organs are sensitively vulnerable to these chemicals. In this work, we have investigated the renal anti‐apoptotic efficiency of linseed (LS) against the oxidative stress‐mediated upregulation of the fetal apoptosis‐related genes following the prenatal intoxication with diesel nanoparticles (DNPs) and/or fenitrothion (FNT). A fifty‐six timed‐pregnant rats were equally divided to eight groups; control, LS (20% in diet), DNPs (0.5 mg/kg by intratracheal inoculation), FNT (3.76 mg/kg by gavage), DNPs+FNT, LS + DNPs, LS + FNT, and LS + DNPs+FNT. The transmission electron microscope analysis revealed the spherical shape of diesel particles with a homogeneous nanosized range (20–92.3 nm) and the crystallinity was confirmed by electron diffraction microscopy. Administration of DNPs and/or FNT significantly increased fetal renal malondialdehyde, nitric oxide, and glutathione reductase as compared with the control group. However, they declined the level of glutathione together with the activities of glutathione peroxidase, glutathione‐S‐transferase, superoxide dismutase, and catalase. Furthermore, DNPs and/or FNT elicited many histopathological changes in fetal renal cells, markedly up‐regulated apoptosis‐related gene expressions (p53, p21 caspase‐3, and caspase‐9), and evoked DNA breaks as detected by comet assay. Interestingly, LS supplementation significantly ameliorated the disturbances in oxidant/antioxidant biomarkers, downregulated the apoptosis gene expressions, and alleviated DNA damage alongside renal cell architecture. These findings reveal that the antioxidant and anti‐apoptotic characteristics of LS are acceptable defender pointers for the renal injury especially during gestational exposure to DNPs and/or FNT.

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Evaluation of the effect of serum cystatin-C and ACE I/D and ACE G2350A polymorphisms on kidney function among hypertensive sewage workers

Taha

Shahy

Mahdy-Abdallah

et al. 2020

Environ Sci Pollut Res

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Assessment and modifications of digestion procedures to determine trace elements in urine of hypertensive and diabetes mellitus patients

Cited by 7 publications

References 29 publications

RETRACTED: Occupational exposure to heavy metal dust and its hazardous effects on non-ferrous foundry workers' health

RETRACTED: Occupational exposure to heavy metal dust and its hazardous effects on non-ferrous foundry workers' health

Linseed ameliorates renal apoptosis in rat fetuses induced by single or combined exposure to diesel nanoparticles or fenitrothion by inhibiting transcriptional activation of p21/p53 and caspase‐3/9 through pro‐oxidant stimulus

Evaluation of the effect of serum cystatin-C and ACE I/D and ACE G2350A polymorphisms on kidney function among hypertensive sewage workers

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