Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders

Buch, ArchanaC; Patro, Namrata; Naik, MuktaD; Vimal, Shruti; Chandanwale, ShirishS

doi:10.4103/mjdrdypu.mjdrdypu_46_20

Cited by 3 publications

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“…Along with the times and technology, hematology examinations, including platelet counts, have now progressed from the use of manual methods to automated methods and tools called Hematology analyzers. A hematology analyzer is an automatic tool for complete blood count (CBC) that has high speed and accuracy and has more parameters that can be examined, such as leukocyte type count, erythrocyte index, and platelet index [4], [5]. The use of automated tools will have a direct impact on the accuracy of the results and the efficiency of the examination time [6].…”

Section: Introductionmentioning

confidence: 99%

Compatibility Between Platelet Histogram With Ip Message and Platelet Morphology in Thrombocytopenia Patients

Dewa Ayu Puja Satya Dinarta,

Anik Handayati,

Syamsul Arifin

2023

International Journal of Advanced Health Science and Technology

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Thrombocytopenia is a condition characterized by low levels of platelets in the blood. The platelet count can be checked using a Hematology analyzer, which is an automatic tool for checking a Complete Blood Count (CBC). The principle of this tool is to detect a signal that is comparable to the cell size that has been set on the tool. The results of the CBC examination are accompanied by a histogram and IP Message if there are abnormal cells, so a confirmatory test using a peripheral blood smear is necessary. The purpose of this study was to determine the suitability of the results of the platelet histogram with the IP Message on the platelet morphology of peripheral blood smears of thrombocytopenia patients. This research is a quantitative descriptive study of 54 samples taken using the Saturation Sampling technique at Grati Pasuruan Hospital using the Sysmex XN-1000 from April 1–30, 2023. The variables in this study are histogram results accompanied by IP Messages and platelet morphology on peripheral blood smears. The results of this study are that the histograms that often appear are abnormal heights on the PU Flag and the IP Messages that often appear are “PLT Clumps?”, with a suitable level of platelet morphology on peripheral blood smear examination reaching 90.7%, so it can be concluded that there is a compatibility between the results of the platelet histogram accompanied by the IP Message on the platelet morphology of the peripheral blood smear of thrombocytopenia patients.

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Section: Introductionmentioning

confidence: 99%

Compatibility Between Platelet Histogram With Ip Message and Platelet Morphology in Thrombocytopenia Patients

Dewa Ayu Puja Satya Dinarta,

Anik Handayati,

Syamsul Arifin

2023

International Journal of Advanced Health Science and Technology

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The dilution evaluation as a corrective measure for interference in the white blood cell scattergram in Beckman Coulter DxH 900

Freitas,

Reis,

Oliveira

et al. 2024

Clinical Laboratory Analysis

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BackgroundThe Beckman Coulter DxH 900 is a haematological analyser capable of counting and sizing blood cells, and obtaining a complete blood cell count (CBC). This analyses different parameters of red blood cells (RBC), platelets and white blood cells/leukocytes. Some automated CBC counters present limitations due to specimen characteristics, abnormal cells or both factors. In the presence of abnormalities, the DxH 900 has a flagging system, warning the laboratory technician that something needs to be verified. In the present work, we evaluated samples from oncologic patients, presenting a population erroneously perceived as being lymphocytes. The most common explanations for this situation are RBC resistant to lysis or serum hyperbilirubinaemia.MethodsIn an attempt to solve and understand what the cause of this problem might be, we diluted our samples (1:3) and analysed the serum total bilirubin. To identify cells' abnormalities, the samples were also analysed by manual DLC counts. During the study, we also checked the different flags presented by the equipment.ResultsThe results evidenced that the major interference was due to RBC lysis resistance, corresponding to 94.7% of the cases, while hyperbilirubinaemia was only present in 73.4%. Besides, we determined that some samples with normal bilirubin levels also presented interference, suggesting that hyperbilirubinaemia was not the main cause of the error. The most recurrent flag observed was “High event rate”.ConclusionThe dilution solved all of the observed interferences. The results between diluted and manual counts showed a strong correlation, leading us to introduce dilution in our laboratory routine.

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The Correlation of Red Cell Distribution Width With Peripheral Blood Smear: A Study From a Tertiary Care Hospital in Peshawar

Abbas,

Shah,

Iftikhar

2024

Cureus

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Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders

Cited by 3 publications

References 1 publication

Compatibility Between Platelet Histogram With Ip Message and Platelet Morphology in Thrombocytopenia Patients

Compatibility Between Platelet Histogram With Ip Message and Platelet Morphology in Thrombocytopenia Patients

The dilution evaluation as a corrective measure for interference in the white blood cell scattergram in Beckman Coulter DxH 900

The Correlation of Red Cell Distribution Width With Peripheral Blood Smear: A Study From a Tertiary Care Hospital in Peshawar

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