Assessment by flow cytometry of peripheral blood leukocyte enzymatic activities in HIV patients

Prin-Mathieu, Christine; Baty, Vincent; Fauré, G; Schumacher, Hélène; Kolopp‐Sarda, Marie‐Nathalie; May, Thierry; Canton, P.; Béné, Marie‐Christine

doi:10.1016/s0022-1759(01)00348-9

Cited by 7 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Elastase and cathepsin G play an important role in such inflammatory diseases as arthritis, as demonstrated by the fact that neutrophil elastase Ϫ/Ϫ or cathepsin G Ϫ/Ϫ mice are resistant to arthritis induction (1). In another study, we have also demonstrated alterations of enzymatic activities in human immunodeficiency virus-infected patients, who display lowered collagenase and cathepsin D activities in monocytes and increased elastase and cathepsin D activities in PMNs compared to controls (30).…”

mentioning

confidence: 62%

Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide

Prin-Mathieu¹,

Roux

Fauré³

et al. 2002

Clin Vaccine Immunol

View full text Add to dashboard Cite

Leukocytes are recruited from peripheral blood into milk as part of the inflammatory response to mastitis. However, excessive accumulation of inflammatory cells alters the quality of milk and the proteases produced by polymorphonuclear neutrophils (PMNs) and macrophages may lead to mammary tissue damage. To investigate PMN recruitment and the kinetics of their intracytoplasmic enzymes in inflammation, we generated mastitis in six cows by intramammary infusion of lipopolysaccharide (LPS). Clinical signs of acute mastitis were observed in all of the cows, and normal status was resumed by 316 h. Intracytoplasmic elastase, collagenase, and cathepsin activities were measured within live cells by flow cytometry in peripheral blood leukocytes and milk PMNs before and during the inflammatory process (at 10 time points between 4 and 316 h). The proportion of immature PMNs was appreciated by CD33 surface labeling measured in flow cytometry. Leukopenia was observed in the peripheral blood 4 h postinfusion, concomitant to an increase in somatic cell counts in milk. CD33؉ PMNs were preferentially recruited from the peripheral blood to milk. Enzymatic activities were detected in PMNs, lymphocytes, and monocytes at levels depending on the cell type, sample nature, and time of collection. Milk PMNs had lower enzymatic activities than peripheral blood PMNs. This study showed that milk PMNs recruited during LPS-induced experimental mastitis have an immature phenotype and significantly lower enzymatic activities than peripheral blood PMNs. This suggests that CD33, an adhesion molecule, may be involved in the egress from blood to milk and that the enzymatic contents of PMNs are partly used during this process.

show abstract

mentioning

confidence: 62%

Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide

Prin-Mathieu¹,

Roux

Fauré³

et al. 2002

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…Cathepsins are located in the acid endosomal/lysosomal (low pH) compartment of dendritic cells and play a pivotal role in antigen processing (Riese, 2000). Previous studies have shown that cathepsins are associated with viral entry, dissemination and enhanced viral replication (El Messaoudi, 1999, 2000; Prin-Mathieu, 2001; Tscherene, 2006). Polymorphonuclear cells isolated from HIV-1 positive patients have significantly higher cathepsin D activity than cells from normal controls (Prin-Mathieu, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that cathepsins are associated with viral entry, dissemination and enhanced viral replication (El Messaoudi, 1999, 2000; Prin-Mathieu, 2001; Tscherene, 2006). Polymorphonuclear cells isolated from HIV-1 positive patients have significantly higher cathepsin D activity than cells from normal controls (Prin-Mathieu, 2001). Vaginal secretions contain cathepsin D and exposure of lymphocyte cultures to these secretions increased HIV-1 replication in these cells (El Messaoudi 1999, 2000).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analyses of the Effects of Drugs of Abuse on Monocyte-Derived Mature Dendritic Cells

Reynolds

Mahajan

Aalinkeel

et al. 2009

Immunological Investigations

View full text Add to dashboard Cite

Drug abuse has become a global health concern. Understanding how drug abuse modulates the immune system and how the immune system responds to pathogens associated with drug abuse, such hepatitis C virus (HCV) and human immunodeficiency virus (HIV-1), can be assessed by an integrated approach comparing proteomic analyses and quantitation of gene expression. Twodimensional (2D) difference gel electrophoresis was used to determine the molecular mechanisms underlying the proteomic changes that alter normal biological processes when monocyte-derived mature dendritic cells were treated with cocaine or methamphetamine. Both drugs differentially regulated the expression of several functional classes of proteins including those that modulate apoptosis, protein folding, protein kinase activity, and metabolism and proteins that function as intracellular signal transduction molecules. Proteomic data were validated using a combination of quantitative, real-time PCR and Western blot analyses. These studies will help to identify the molecular mechanisms, including the expression of several functionally important classes of proteins that have emerged as potential mediators of pathogenesis. These proteins may predispose immunocompetent cells, including dendritic cells, to infection with viruses such as HCV and HIV-1, which are associated with drug abuse. KeywordsCocaine; Methamphetamine; Monocyte-derived mature dendritic cells; Difference gel electrophoresis (DIGE); High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

show abstract

“…RGES (Arginine-Glycine-Glutamicacid-Serine-Rho110) includes a part of fibronectin, which is a substrate of PMN elastase. When the bond between the leaving group and the dye is cleaved by the PMN elastase, fluorescence is released (Prin-Mathieu et al, 2001). The assay procedure was the same as DCFH, PMA oxidative burst test.…”

Section: Determination Of Pmn Elastase Activity By Flow Cytometric Rges-elastase Testmentioning

confidence: 99%

“…CellProbe reagents consist of synthetic nonfluorescent enzymatic substrates that are characterized by their low molecular weight and high polarity. This allows them to diffuse through the cytoplasmic membrane of living cells without previous permeabilization (Prin-Mathieu et al, 2001).…”

mentioning

confidence: 99%

Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst

Barlak¹,

Uçar²,

Cakıroglu³

2015

Turk J Biol

View full text Add to dashboard Cite

show abstract

Assessment by flow cytometry of peripheral blood leukocyte enzymatic activities in HIV patients

Cited by 7 publications

References 13 publications

Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide

Enzymatic Activities of Bovine Peripheral Blood Leukocytes and Milk Polymorphonuclear Neutrophils during Intramammary Inflammation Caused by Lipopolysaccharide

Proteomic Analyses of the Effects of Drugs of Abuse on Monocyte-Derived Mature Dendritic Cells

Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst

Contact Info

Product

Resources

About