Assessment of a commercially available point-of-care assay for the measurement of bovine cardiac troponin I concentration

Abstract

Results of this study indicated the point-of-care assay had high precision for determination of cTnI concentrations in most evaluated prepared bovine plasma standard samples. The point-of-care assay may be useful for determination of circulating concentrations of cTnI in cattle.

Measurement of cardiac troponin I in healthy lactating dairy cows using a point of care analyzer (i-STAT-1)

“…10,14 However, since cTnI testing is expensive in a food animal context (w$35 per test), most studies focusing on cTnI quantification have included a small number of samples from healthy animals and commonly performed only descriptive analysis of their healthy animal datasets. 4,5 Using the bootstrap method allowed the generation of a high number of datasets using unrestricted random resampling of the initial dataset. 15 This technique allows a non-biased estimate determination of the confidence interval of a parameter within a population.…”

“…6 Assessment of the blood concentration of cTnI in healthy animals using the i-STAT 1 immunoassay has been reported recently in adult cows using serum samples 5 and in calves. 4 Unfortunately, no reference interval was mentioned; only the observed values were presented. The observed values in serum samples were lower than 0.02 ng/ mL in 30 cows of different breeds (Jersey and Holstein) and in different physiological statuses (20 lactating cows, 10 dry cows).…”

Prognostic Value of Cardiac Troponin I and L‐Lactate in Blood of Dairy Cows Affected by Downer Cow Syndrome

“…A blood sample collected in a lithium‐heparin tubeb was used to quantify blood cTnI c. Use of bovine whole blood with this analyzer has been validated . This portable immunoassay analyzer uses a two‐site ELISA monoclonal antibody (caprine, murine) directed toward human cTnI.…”

Assessment of Blood Concentration of Cardiac Troponin I (cTnI) in Healthy Calves and Stocker Calves Effected with Bovine Respiratory Disease Complex

“…Cardiac troponin I (cTn I) has become a common test for myocardial injury in people and is used in veterinary medicine for similar purposes [12][13][14][15][16][17][18][19][20]. Troponin I assays are increasingly available in table-top or hand-held biochemistry analyzers [21]. This presents an opportunity for field testing of large groups of cattle with rapid results that can aid in disease classification and outcome discrimination.…”

“…This presents an opportunity for field testing of large groups of cattle with rapid results that can aid in disease classification and outcome discrimination. Recently, serum cTn I has been reported for cattle having a variety of cardiogenic and non-cardiogenic disease [21][22][23][24][25][26][27].…”