Tile drainage effluent from agriculture fields is beneficial to production agriculture; however, nitrate and phosphate transport from production fields to surface water resources is an environmental concern. The David M. Barton Agriculture Research Center (Cape Girardeau County, Missouri, USA) has a 40 ha controlled subsurface tile drainage/irrigation technology with associated denitrification bioreactor. Nitratebearing effluents from the controlled subsurface tile drainage/irrigation technology under a corn (Zea mays L.)-soybean (Glycine max L) rotation is sufficient to be an environmental concern. Nitrate-bearing effluent passage through the denitrification bioreactor typically promotes sufficient nitrate reduction (denitrification) that the bioreactor effluent water is less than 10 mg NO 3-N/L. Phosphorus, ammonium-N, and sulfate-S concentrations are not appreciably influenced by denitrification bioreactor passage.