Assessment of a Thermostable Xylanase from Paenibacillus sp. ASCD2 for Application in Prebleaching of Eucalyptus Kraft Pulp

Chapla, Digantkumar; Patel, Harshvadan; Madamwar, Datta; Shah, Amita

doi:10.1007/s12649-012-9112-z

Cited by 19 publications

(8 citation statements)

References 21 publications

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“…Use of xylanase can significantly reduce the amount of chlorine used for bleaching of pulp (Viikari et al 1986 ; Paice et al 1988 ; Clarke et al 1991 ; Jeffries et al 1992 ) Biobleaching of eucalyptus kraft pulp using xylanase from B. pumilus ASH5 resulted in reduction of chlorine and chlorine dioxide consumption by 20 and 10 %, respectively, besides improved brightness and whiteness of pulp (Battan et al 2007 ). Chapla et al ( 2012 ) reported similar result using cellulose free thermostable xylanase from Paenibacillus sp. ASCD2 in prebleaching of eucalyptus kraft pulp.…”

Section: Application Of Xylanasesmentioning

confidence: 61%

“…Xylanases are produced by fungi, bacteria, yeast, marine algae, protozoa, snails, crustaceans, insect, seeds, etc. (Polizeli et al 2005 ) Bacterial genera, such as Bacillus , Cellulomonas , Micrococcus , Staphylococcus , Paenibacillus , Arthrobacter , Microbacterium , Pseudoxanthomonas, and Rhodothermus have been reported to produce xylanases (Subramanian and Prema 2000 ; Beg et al 2001 ; Gupta et al 2001 ; Chapla et al 2012 ). Among the actinomycetes group, Streptomyces, Actinomadura , Nonomuraea , etc.…”

Section: Xylanolytic Bacteria and Their Xylanasesmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial xylanases: biology to biotechnology

et al. 2016

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In this review, a comprehensive discussion exclusively on bacterial xylanases; their gene organization; different factors and conditions affecting enzyme yield and activity; and their commercial application have been deliberated in the light of recent research findings and extensive information mining. Improved understanding of biological properties and genetics of bacterial xylanase will enable exploitation of these enzymes for many more ingenious biotechnological and industrial applications.

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Section: Application Of Xylanasesmentioning

confidence: 61%

Section: Xylanolytic Bacteria and Their Xylanasesmentioning

confidence: 99%

Bacterial xylanases: biology to biotechnology

et al. 2016

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“…Xylanase producing bacterial strains were isolated from different kind of areas, such as Antarctic environments [18], marines [17], soda lakes [30] and thermal springs [16]. Microbial production of xylanases from bacteria, such as Bacillus, Cellulomonas, Micrococcus, Staphylococcus, Paenibacillus, have been reported [9,31,32,33]. Because thermostability is a desired characteristic, thermostable xylanases have a great importance among industrial enzymes.…”

Section: Xylanase Activitymentioning

confidence: 99%

Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey

Çolak

İnan

Tokgöz³

et al. 2018

Sakarya University Journal of Science

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The aim of this study was screening of xylanase and glucose isomerase producing thermophilic bacteria isolated from some hot springs located in Aegean Region of Turkey. Total sixty eight thermophilic isolates (Anoxybacillus, Brevibacillus, Geobacillus, Aneurinibacillus, Thermus, Paenibacillus and Proteobacter) were collected previously from these fields and identified based on 16S rDNA gene sequences. Isolates were screened by plate assay for determining the xylanase and glucose isomerase production abilities seperately in order to find new strains for industrial processes. After an incubation period of two days for xylanase and 5-6 days for glucose isomerase at 50-60 °C, positive isolates were determined. Enzyme producing isolates were confirmed by spectrophotometric measurements with crude enzyme extracts, birchwood xylan and glucose were used as substrates. Most of the isolates (fifty nine) were positive for xylan degradation while only sixteen of them were positive for glucose isomerase activity. Fourteen of the isolates showed both xylanase and glucose isomerase activity. None of the isolates belong to the genera Paenibacillus, Aneurinibacillus, and Proteobacter were glucose isomerase positive, although the glucose isomerase activity of Geobacillus isolates were notably high. Both xylanase and glucose isomerase activities were observed at 50-60 °C which is suitable for biotechnological applications.

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“…xylanases (1,EC 3.2.1.8. xyl) are hydrolytic enzymes, which is being commercially important industrial enzyme. It can withstand at certain level of alkalinothermophilics conditions but depends on its source organism (Chapla et al, 2012;Saleem et al, 2012).…”

Section: Extra-cellularmentioning

confidence: 99%

Stable activity of extra-cellular xylanases and its phylogeny in different Bacillus species

2017

Int. J. Biosci.

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The production of cellulose free xylanase has increased its demand for waste treatment in pulping and bleaching industrial processes. Four Bacillus strains were selected on the basis of their habitation for the production of xylanase in xylan based nutrient culture. The secreted xylanase was referenced with protein marker for size of 20.3kDa in B. subtilis 168, 20.4kDa in B. pumilus, 28.6kDa B. cereus and 23.3kDa in B. amyloliquefaciens on SDS-PAGE. High activity of xylanase was observed in B. pumilus than B. amyloliquefaciens and B. cereus under harsh extremophillic culture conditions like as 28ºC with pH 4.0 and 50ºC with pH 10.0. The phylogenetic tree shows divergence of xylanase produce by B. pumilus from other 4 Bacillus species because of its evolution in proteins sequence for adaptation to severe habitate-conditions. In optimum culture medium, maximum cell biomass produced by B. pumilus (p ≤0.05) and significantly higher total extra-cellular protein contents in B. subtilis cultures. It might be permissibly nice, if origin of xylanases could be excised from B. pumilus and produced with B. subtilis 168 that could bring a revolution in pulp and paper industry. Preference for B. subtilis 168, it is being a safe bacterium for neighborhood.

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Assessment of a Thermostable Xylanase from Paenibacillus sp. ASCD2 for Application in Prebleaching of Eucalyptus Kraft Pulp

Cited by 19 publications

References 21 publications

Bacterial xylanases: biology to biotechnology

Bacterial xylanases: biology to biotechnology

Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey

Stable activity of extra-cellular xylanases and its phylogeny in different Bacillus species

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