Assessment of a two-step high-performance liquid chromatographic assay using dual-wavelength ultraviolet monitoring for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol in human serum or plasma

Norris, Ross; Thomas, Mansel J.; Craswell, Peter W.

doi:10.1016/s0378-4347(00)83564-1

Cited by 20 publications

(7 citation statements)

References 13 publications

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“…showed a correlation of 0.89 [15] and 0.94 [16] between HPLC and CPBA for total serum 25OHD measurement, which is similar to our observation. In a recent study conducted to assess the inter-laboratory variation for the measurement of serum 25OHD, Lips et al [17] reported that the mean serum 25OHD was about 80% higher when measured by CPBA than by HPLC.…”

Section: Resultssupporting

confidence: 91%

Effect of Vitamin D supplement use on serum concentrations of total 25OHD levels in elderly women

Rapuri¹,

Gallagher²

2004

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Resultssupporting

confidence: 91%

Effect of Vitamin D supplement use on serum concentrations of total 25OHD levels in elderly women

Rapuri¹,

Gallagher²

2004

The Journal of Steroid Biochemistry and Molecular Biology

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“…This method does not distinguish between 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3. However, it has been shown previously by Norris et al (1986) using an HPLC procedure that the concentration of 25-hydroxyvitamin D2 is less than 5 nmol/l in samples taken from elderly residents at the same institution irrespective of total 25(OH)D. Interassay CVs were derived from 1 2 assays performed over 4 months using low and high 25(OH)D concentrations (low mean 27.6 nmol/l, CV 7.2%; high mean 180.0 nmol/l, CV 5.0%).…”

Section: (Oh)d Measurementsmentioning

confidence: 85%

The influence of sunlight or dietary vitamin D on plasma 25‐hydroxyvitamin D in institutionalized elderly patients in a sub‐tropical climate

Lovell

Byth

Craswell

et al. 1988

J Human Nutrition Diet

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Plasma 25-hydroxyvitamin D (25(OH)D) concentrations in a group of thirtyeight nursing-home residents (age range 55-95 years) living in a sub-tropical climate (Brisbane, Australia) were less than 25 nmol/l in 50% of cases. The effects of endogenously and exogenously derived vitamin D have been studied over three months (April-June) using the following treatment groups: A, placebo; B, daily sun exposure; C, 18.8 nmol vitamin D/day and D, 56.3 nmol vitamin D/day. Exposure to ultra-violet radiation of group B patients was monitored by meter and palysulphone film. one month of treatment and groups B and C, respectively, after two months (P

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“…A study assessed the stability of different vitamin D metabolites including 1,25OHD and 24,25OHD reported that 1,25OH in serum is stable for 57 days at −80 °C, 24h at 4 °C, and unstable at room temperature [28]. Similarly, older studies investigated the stability of vitamin D in samples stored at -20 °C showed 10% decrease in vitamin D concentration after 3 months [29,30]. On the other hand, a study evaluated a short term vitamin D stability in serum and plasma samples reported no significant change at 24 °C during 72 hours [31].…”

Section: Influence Of Temperature On Vitamin D Metabolitesmentioning

confidence: 99%

Pre-Analytical Factors Influence Vitamin D Measurement In Clinical Laboratory

Alnagar

2018

int.jour.sci.res.mana

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Abstract:It has been more than 90 years since the discovery of vitamin D and its capability to cure rickets in children. Recently, awareness is growing of the prevalence of vitamin D deficiency in the general population in association with an increased risk of several diseases. Analyses of vitamin D levels in blood samples provide direct measures of exposure to dietary or supplemented vitamin. This review is focused on the pre-analytical factors and challenges are known to affect the concentrations of vitamin D biomarkers. The pre-analytical measurement of disease relevant vitamin D deficiency in blood can vary as a function of differences in sample handling and processing. It could impact the accurate measurement of the diseases relevant vitamin D deficiency. Majority of the analysis in a clinical biochemistry laboratory requires serum or plasma as the analytical specimen and obtaining serum or plasma from a primary sample is an important step of pre-analytical procedure. Although vitamin D is considered as a relatively stable analyte, effect of pre-analytical conditions and stability of vitamin D in serum and plasma needs to be identified clearly.

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Assessment of a two-step high-performance liquid chromatographic assay using dual-wavelength ultraviolet monitoring for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol in human serum or plasma

Cited by 20 publications

References 13 publications

Effect of Vitamin D supplement use on serum concentrations of total 25OHD levels in elderly women

Effect of Vitamin D supplement use on serum concentrations of total 25OHD levels in elderly women

The influence of sunlight or dietary vitamin D on plasma 25‐hydroxyvitamin D in institutionalized elderly patients in a sub‐tropical climate

Pre-Analytical Factors Influence Vitamin D Measurement In Clinical Laboratory

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