Assessment of acid phosphatase enzyme and influence of potassium iodide on its production in the yeast form of Sporothrix schenckii

Abstract

Background: Sporotrichosis is caused by a dimorphic fungal species, Sporothrix schenckii (S. schenckii). The enzyme acid phosphatase is pervasive among yeast and yeast like fungi. It has been studied in various fungi like Aspergillus oryzae, Candida albicans etc. but in S. schenckii little is known about enzyme acid phosphatase. The present study depicts the in-vitro influence of Potassium Iodide (KI) on the enzyme acid phosphatase produced by the S. schenckii (yeast form).Methods: A master culture was prepared by incorporating the standard strain of S. schenckii in YNB (Yeast Nitrogen Base) medium and was incubated at 37ºC. After preparing the increasing concentrations with KI in YNB medium, 1.0 mL suspension of master culture was inoculated into each bottle and incubated at 37ºC for different time period 6th, 12th, 18th day (early, mid, peak of log period) respectively. After centrifuging, a 5% homogenate was prepared, which was used for acid phosphatase enzyme assay.Results: The mean acid phosphatase level of control specimen was 20.9±2.01, 50.0±2.25, 45.0±5.10 μg and test specimens was ranged from 14.9±4.89 to 20.2±3.49, 10.2±4.19 to 40.0±6.39 and 10.0±1.81 to 34.7±6.08 μg on day 6, 12 and 18 respectively. The mean value was lower significantly for all the test concentrations as compared to control (p<0.05).Conclusions: The low activity of the enzyme acid phosphatase indicates that KI has inhibitory effect on the growth of S. schenckii that has led to decrease in the activity of the enzyme.

Assessment of transaminase enzymes and effect of potassium iodide on its production in the yeast form of Sporothrix schenckii

“…[13][14][15][16] S. schenckii was sub-cultured from the slope of Sabouraud's dextrose agar (SDA) in 50 mL of YNB (Yeast Nitrogen Base, HiMedia, Mumbai) medium, which was placed in a screw-capped bottle and incubated at 37ºC to create a master culture. After injecting the right amount of YNB medium on day seven, the growth of S. schenckii in the bottle was brought to 90% transmission at 540 nm, following the instructions provided by Bareja et al 6 The resulting master culture was used for further study. The YNB medium was produced and then added to 150 screw-capped bottles with a 160 mL capacity in 50 mL aliquots.…”

“…Several fungi include transaminases, also known as aminotransferases, which are a component of protein. 6 Aspartate aminotransferase (AST) and alanine aminotransferase (ALT), transaminases that cause sporotrichosis, have not been studied in relation to S. schenckii. Since the early 20 t h century, potassium iodide (KI) has been used traditionally to treat sporotrichosis with excellent results; nevertheless, the precise mechanism of action is still unknown.…”

Expression of transaminase enzymes and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii

“…A 5% homogenate was prepared from each weighed tissue in ice-cold distilled water and was used for subsequent enzyme assay. 1,4 The transaminases enzymes, aspartate aminotransferase and alanine aminotransferase were determined by the method of Reitman and Frankel. 5 The enzymes AST and ALT were estimated in per mg wt.…”

“…lymphocutaneous, localized cutaneous, disseminated, mucosal, skeletal and visceral. 1 Pathogenic potential of S. schenckii is not as much known on mammalian host due to scarcity of enzymatic information. Transaminases or aminotransferases being a part of protein is found in various fungi.…”