Assessment of Acid Phosphatase Production by In vitro Cultures of Atropa acuminata

Abstract

The potential of various culture lines of Atropa acuminata were investigated for resourcing acid phosphatase (ACP) (3.1.3.2). Crude enzyme extract comprised of a mixture of four isoforms, distinguishable by polyacrylamide gel electrophoresis (PAGE) with molecular weight ranging from 39 to 215 kDa. In vitro regenerated proliferative shoots, callus and roots showed higher specific activity (2.49, 3.41, 2.91 U/mg protein, respectively) as compared to in vivo grown plants (0.71 U/mg protein). ACP activity in root cultures increased progressively up to 4.6 U/mg during the entire growth period (2 -24 weeks), whereas in case of shoot cultures, the specific activity escalated to 2.49 U/mg at 8 weeks, which then declined subsequently (1.95 U/mg). Similarly, callus cultures initially showed a higher phosphohydrolytic activity (3.41 U/mg protein) until 8 weeks by which period, it decreased with the passage of growth period. The present studies reveal an alternate system for resourcing of ACP from Atropa acuminata.

Dynamics of acid phosphatase production by cell suspension system and its further characterization

“…Seedlings of A. acuminata were transferred aseptically into MS media and different morphogenetic lines were regenerated as reported earlier (Ahuja et al, 2002). Nondifferentiated callus culture with higher enzyme activity (Khan et al, 2016) was exploited for further studies.…”

“…Since static callus cultures exhibited the highest APase activity during early stages of culture growth (Khan et al, 2016), thus it was developed as a progressively proliferating suspension culture by culturing the cells in MS media containing NAA and BAP (1 mgL -1 ) for further experimentations to scale up the production of APase.…”