Assessment of acute pancreatitis severity via determination of serum levels of hsa‑miR‑126‑5p and IL‑6

Chen, Yingjie; Lin, Tianlai; Cai, Zhe; Yan, Chang-Hu; Gou, Sen-Ren; Zhuang, Yaodong

doi:10.3892/etm.2020.9458

Cited by 15 publications

(16 citation statements)

References 35 publications

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“…A multicenter trial revealed that at a PCT value of 3.8 ng/ml within 96 h after the onset of symptoms, diagnostic sensitivity and specificity for pancreatic necrosis or death were 93% and 79%, respectively 29 . Some studies have also confirmed the potential value of IL‐6 as a prognostic indicator in SAP 30,31 . However, to the best of our knowledge, to date, only 1 study published in 2020 has reported the use of HBP to predict persistent organ failure in AP patients with a specificity of 74%, a sensitivity of 90%, and an AUC of 0.82 25 .…”

Section: Discussionmentioning

confidence: 99%

“… 29 Some studies have also confirmed the potential value of IL‐6 as a prognostic indicator in SAP. 30 , 31 However, to the best of our knowledge, to date, only 1 study published in 2020 has reported the use of HBP to predict persistent organ failure in AP patients with a specificity of 74%, a sensitivity of 90%, and an AUC of 0.82. 25 Our study revealed that the concentrations of analyzed biomarkers were all significantly higher in deaths than in survivors, suggesting that elevated levels were important risk factors for the development of AP.…”

Section: Discussionmentioning

confidence: 99%

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Combined detection of procalcitonin, heparin‐binding protein, and interleukin‐6 is a promising assay to diagnose and predict acute pancreatitis

Zhong

Wang

et al. 2021

Clinical Laboratory Analysis

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Background Acute pancreatitis (AP), one of the most common clinical emergencies, is characterized by variable clinical features and inadequate diagnostic methods. At present, the commonly used indicators do not have high specificity and do not necessarily reflect disease severity. We therefore aimed to investigate diagnostic and prognostic value of plasma procalcitonin, heparin‐binding protein, and interleukin‐6 for acute pancreatitis by separate detection and joint detection. Methods The study involved 451 participants, including 343 AP patients and 108 healthy controls. We analyzed the association of the three biomarkers with the severity and prognosis of AP. Results A statistically significant increase in the mean plasma analyte levels was detected in the study group compared to the control group. Multivariate comparison showed that plasma levels of PCT, HBP, and IL‐6 were all significantly different among the three groups at different sampling times (1st, 3rd, 7th, and 10th day of admission) (p < 0.01). The combination of the three indicators had significantly higher diagnostic value than either the individual markers or pairwise combinations (p < 0.001). The levels of the three were all significantly higher in severe acute pancreatitis (SAP) patients than in non‐SAP patients (p < 0.001); meanwhile, patients with high levels had a worse prognosis than those with low levels (p < 0.05). In multivariate analysis adjusted for age and sex, high levels of PCT, HBP, and IL‐6 were found to be independently associated with the development of AP. Conclusions It dramatically improved the diagnostic power of AP when PCT, HBP, and IL‐6 were combined; high PCT, HBP, and IL‐6 levels within 3 days of admission may be the potentially useful indicators for predicting SAP.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Combined detection of procalcitonin, heparin‐binding protein, and interleukin‐6 is a promising assay to diagnose and predict acute pancreatitis

Zhong

Wang

et al. 2021

Clinical Laboratory Analysis

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“…In the study, IL-10 production was increased in diabetic BMC when compared with wt BMC (Figure 5 AP [22]. IL-10 is produced by activated T cells such as Treg, Th17, and Th1 cells and macrophages stimulated by bacterial lipopolysaccharides and catecholamines or clearance of apoptotic cells [23].…”

Section: Discussionmentioning

confidence: 79%

Diabetic Bone Marrow Cell Injection Accelerated Acute Pancreatitis Progression

Luo

Yan

Zhang

et al. 2021

Journal of Immunology Research

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Acute pancreatitis (AP) is one of the leading causes of hospital admission, 20% of which could progress to the severe type with extensive acinar cell necrosis. Clinical studies have reported that diabetes is an independent risk factor of the incidence of AP and is associated with higher severity than nondiabetic subjects. However, how diabetes participates in AP progression is not well defined. To investigate this question, wild-type (wt) and diabetic db/db mice at the age of 16 weeks were used in the study. AP was induced in wt recipients by 10 injections of 50 μg/kg caerulein with a 1 h interval. One hour after the last caerulein injection, bone marrow cells (BMC) isolated from wt and db/db mice were injected intraperitoneally into the recipients ( 1 × 10 7 cells/recipient). The recipients with no BMC injection served as controls. Thirteen hours after BMC injection, serum lipase activity was 1.8- and 1.3-folds higher in mice that received db/db BMC, compared with those with no injection and wt BMC injection, respectively ( p ≤ 0.02 for both). By H&E staining, the overall severity score was 14.7 for no cell injection and 16.6 for wt BMC injection and increased to 22.6 for db/db BMC injection ( p ≤ 0.002 for both). In particular, mice with db/db BMC injection developed more acinar cell necrosis and vacuolization than the other groups ( p ≤ 0.03 for both). When sections were stained with an antibody against myeloperoxidase (MPO), the density of MPO+ cells in pancreatitis was 1.9- and 1.6-folds higher than wt BMC and no BMC injection groups, separately ( p ≤ 0.02 for both). Quantified by ELISA, db/db BMC produced more IL-6, GM-CSF, and IL-10 compared with wt BMC ( p ≤ 0.04 for all). In conclusion, BMC of db/db mice produced more inflammatory cytokines. In response to acinar cell injury, diabetic BMC aggravated the inflammation cascade and acinar cell injury, leading to the progression of acute pancreatitis.

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“…The severity of AP is associated with increased serum levels of inflammatory cytokines markers including TNF‐α, IL‐1β, IL‐6, IL‐8, and IL‐10 (Chen et al, 2021). Furthermore, IL‐6 is identified as a significant inflammatory cytokine marker in the development of AP (Ćeranić et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

TSPAN1 silencing protects against cerulein‐induced pancreatic acinar cell injury via targeting AGR2

Wang

Huang

2021

Drug Development Research

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Acute pancreatitis (AP) is an inflammatory gastrointestinal disorder affecting the pancreas. Previous study reported that tetraspanin 1 (TSPAN1) expression was significantly upregulated in the pancreas of AP patients. However, the underlying molecular mechanism of TSPAN1 in the pathogenesis of AP remains unclear. Thus, the aim of the present study was to investigate the potential role of TSPAN1 in development of AP. RT‐qPCR was carried out to quantify the relative mRNA levels of TSPAN1 and anterior gradient‐2 (AGR2). The CCK‐8 assay was used to detect the cell viability. The TUNEL assay was performed to visualize the apoptotic cells. Western blot was performed to determine the expressions of proteins related to endoplasmic reticulum (ER) stress and apoptosis. ELISA kits were adopted to detect the concentration of inflammatory cytokines including TNF‐α and IL‐6. Finally, immunoprecipitation (IP) was used to verify the interaction between TSPAN1 and AGR2. TSPAN1 was upregulated in serum of AP patients and AP cell models. TSPAN1 silencing promoted the cell proliferation and inhibited inflammatory response in cerulein‐induced AR42J cells. Moreover, TSPAN1 induced endoplasmic reticulum stress by binding AGR2. Interestingly, the overexpression of AGR2 abolished the effects of TSPAN1 silencing on cell proliferation and inflammatory response in cerulein‐induced AR42J cells. In summary, TSPAN1 silencing protects against cerulein‐induced pancreatic acinar cell injury through inhibiting ER stress‐mediated by AGR2. Hence, TSPAN1 may serve as a promising therapeutic target for AP treatment.

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Assessment of acute pancreatitis severity via determination of serum levels of hsa‑miR‑126‑5p and IL‑6

Cited by 15 publications

References 35 publications

Combined detection of procalcitonin, heparin‐binding protein, and interleukin‐6 is a promising assay to diagnose and predict acute pancreatitis

Combined detection of procalcitonin, heparin‐binding protein, and interleukin‐6 is a promising assay to diagnose and predict acute pancreatitis

Diabetic Bone Marrow Cell Injection Accelerated Acute Pancreatitis Progression

TSPAN1 silencing protects against cerulein‐induced pancreatic acinar cell injury via targeting AGR2

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