Assessment of acute renal allograft dysfunction by MRI diffusion techniques

Sherif, Mohamed Fouad; Alghar, Mohamad I. Abu; Alshafe, Mohamad H.; Badra, A

doi:10.1016/j.ejrnm.2018.06.018

Cited by 2 publications

(6 citation statements)

References 19 publications

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“…Cells were cultured with Dulbecco Modified Eagle Media/F12 supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA), 100 U/ml penicillin G, 100 μg/ml streptomycin, and 0.25 μg/ml amphotericin B (Invitrogen) at 37°C in a humidified atmosphere of 5% CO 2 . To construct in vitro ischemia/reoxygenation model, cells were cultured in a hypoxic atmosphere containing 1% O 2 , 94% N 2 , and 5% CO 2 for 3 hours, followed by reoxygenation maintained in complete medium in 21% O 2 at different time points (1,3,6,12, and 24 hours).…”

Section: Renal Morphologymentioning

confidence: 99%

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Inhibition of lncRNA MEG3 protects renal tubular from hypoxia‐induced kidney injury in acute renal allografts by regulating miR‐181b/TNF‐α signaling pathway

Pang

Gao

Shang

et al. 2019

J of Cellular Biochemistry

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Early damage to transplanted organs initiates excess inflammation that deteriorates existing injury, which is a leading cause of graft loss. Long noncoding RNAs (lncRNAs) are recently thought to play a significant role in cellular homeostasis during pathological process of kidney diseases. The aim of this study was to assess the function and mechanism of lncRNA, maternally expressed gene 3 (MEG3), on early renal allografts pathogenesis. Real-time polymerase chain reaction (RT-PCR) analysis found that the levels of MEG3 and miR-181b-5p were increased and decreased respectively in grafted kidney.The Western blot assay showed that TNF-alpha was upregulated in the kidney and in HK-2 cells. Administering MEG3-specific small interfering RNA (siRNA) in mice silenced MEG3 expression and protected kidney renal allograft from injury. Bioinformatical analysis and luciferase assay indicated that MEG3 is a target of miR-181b-5p. MEG3 inhibition and overexpression promoted and suppressed miR-181b-5p levels respectively. In addition, Western blot and immunohistochemical staining suggested that decreased TNF-alpha expression was observed in the kidney. In contrary to MEG3, miR181b overexpression attenuated hypoxia-induced HK-2 cell apoptosis, as well as suppressed hypoxiainduced TNF-alpha upregulation. In luciferase reporter assay, we confirmed that miR-181b directly bound to the 3′-untranslated region (3′-UTR) of TNFalpha, thereby negatively regulating the TNF-alpha expression. Our data suggested that MEG3 functions as a competing endogenous RNA for miR-181b to regulate the TNF-alpha expression in hypoxia-induced kidney injury in acute renal allografts. K E Y W O R D S acute renal allografts, hypoxia-induced kidney injury, maternally expressed gene 3, miR-181b, renal tubular J Cell Biochem. 2019;120:12822-12831. wileyonlinelibrary.com/journal/jcb 12822 |

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Section: Renal Morphologymentioning

confidence: 99%

“…Renal transplantation is considered as primary treatment option for patients with end‐stage kidney disease . Acute rejection is the most lethal cause of early renal allograft failure .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of lncRNA MEG3 protects renal tubular from hypoxia‐induced kidney injury in acute renal allografts by regulating miR‐181b/TNF‐α signaling pathway

Pang

Gao

Shang

et al. 2019

J of Cellular Biochemistry

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“…Table (1) illustrates statistically significant difference between cases with medical and surgical complications as regard conventional technique with higher abnormal findings among cases with medical complications than cases with surgical complications. No statistically significant difference is detected between groups with complications as regard MRI & MRU findings.…”

Section: Resultsmentioning

confidence: 97%

“…RENAL transplantation is the most effective treatment option in patients with end-stage renal disease. Studies have shown that the 5-year survival after renal transplantation is 70%, as compared to 30% survival in patients receiving dialysis [1] .…”

Section: Introductionmentioning

confidence: 99%

“…The most important role of diagnostic radiological methods is to identify multiple complications in the post-transplant period [1] . Surgical complications of renal transplantation include peri-nephric collections in the form of urinomas, lymphoceles and hematomas and Vascular complications such as renal artery stenosis, renal vein thrombosis, renal vein stenosis, renal artery thrombosis, internal arteriovenous fistulas and pseudo aneurysms, external arteriovenous fistulas.…”

Section: Introductionmentioning

confidence: 99%

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Multimodality Radiological Diagnosis of Post Renal Transplantation Complications

GAML¹,

ELHAK

2022

The Medical Journal of Cairo University

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Background: Renal transplantation is the most effective treatment option in patients with end-stage renal disease. Studies have shown that the 5-year survival after renal transplantation is 70%, as compared to 30% survival in patients receiving dialysis.For precise planning, early detection of problems, and workflow effectiveness, it is essential to adopt the proper diagnostic technique in preoperative analysis and also in postoperative follow-up procedure. Aim of Study:To detect the of renal transplant complications by MRI and/or renal scintigraphy compared to renal biopsy in a selected cases.Patients and Methods: This study involved 90 renal transplant patients. There were 63 (70%) male patients and 27 (30%) female patients. They varied in age from 24 to 45 years (mean age 34.5 years). These 90 patients were subdivided into three groups as patients of medical complication (30 patients), patients of surgical complications (30 patients) and Control group of thirty recipient patients. Gray scale Ultrasonography and color Doppler Sonography Applied routinely on all examined transplant recipients. These subjects have been assessed via MRI using 3 Tesla MRI scanner, (Philips, Ingenia).Radio-isotope diuretic renogram by Philips Gamma Camera using Tc99-DTPA radioactive tracer. Performed routinely after 2 weeks from transplantation to all cases as basal study.Results: Study included 90 transplant recipients. That were subdivided into three groups, Group of medical complication Their ages ranged from 20-41 years mean (30.67±9.95) & group of surgical complication; their ages ranged from 23-44 mean (33.90±10.88) and the control group includes 30 transplant recipients normal subjects, their ages ranged from 23-43 years mean (33.47±9.67). Males represented 76.7% and females represented 7% of patients with medical complications. Males represented 73.3% and females represented 26.7% of patients with surgical complications. Males represented 60% and females represented 40% of patients with normal transplanted kidney functions.There is statistically significant lower mean ADC among medical complications than control groups (1.71 versus 2.02,

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Assessment of acute renal allograft dysfunction by MRI diffusion techniques

Cited by 2 publications

References 19 publications

Inhibition of lncRNA MEG3 protects renal tubular from hypoxia‐induced kidney injury in acute renal allografts by regulating miR‐181b/TNF‐α signaling pathway

Inhibition of lncRNA MEG3 protects renal tubular from hypoxia‐induced kidney injury in acute renal allografts by regulating miR‐181b/TNF‐α signaling pathway

Multimodality Radiological Diagnosis of Post Renal Transplantation Complications

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