Assessment of Age-Related Polyploidy in Quercus Robur L. Somatic Embryos and Regenerated Plants Using DNA Flow Cytometry

Endemann, M.; Hristoforoglu, K.; Stauber, Thomas; Wilhelm, Eva

doi:10.1023/a:1012426306493

Cited by 52 publications

(14 citation statements)

References 25 publications

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“…These percentages are in general higher than those reported for other grapevine cultivars by Kuksova et al (1997) and Leal et al (2006). By contrast, Endemann et al (2001) observed a higher percentage of polyploids in Quercus, but it must be taken into consideration that their cultures were 7 years old.…”

Section: Resultscontrasting

confidence: 67%

Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

Prado

Rodriguez²,

Rey

et al. 2010

Plant Cell Tiss Organ Cult

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Flow cytometry and microsatellite analyses were used to evaluate the trueness-to-type of somatic embryogenesis-regenerated plants from six important Spanish grapevine (Vitis vinifera L.) cultivars. Tetraploid plants were regenerated through somatic embryogenesis from all of the cultivars tested with the exception of 'Merenzao'. In addition, an octoploid plant was obtained in the cv. 'Albariño', and two mixoploids in 'Torrontés'. The most probable origin of these ploidy variations is somaclonal variation. The cv. 'Brancellao' presented significantly more polyploids (28.57%) than any other cultivar, but it must be noted that 50% of the adult field-grown 'Brancellao' mother plants analysed were mixoploid. Hence, it is probable that these polyploids originated either from somaclonal variation or by separation of genotypically different cell layers through somatic embryogenesis. Microsatellite analysis of somatic embryogenesis-regenerated plants showed true-totype varietal genotypes for all plants except six 'Torrontés' plants, which showed a mutant allele (231) instead of the normal one (237) at the locus VVMD5. There was not a clear relationship between the occurrence of the observed mutant regenerated plants and the callus induction media composition, the developmental stage of the inflorescences, the type of explant used for starting the cultures or the type of germination (precocious in differentiation medium or normal in germination medium) in any of the cultivars tested, except 'Torrontés'. The mutant plants described herein have been transplanted to soil for future evaluation of putative phenotypic traits of interest. These mutants can be useful both for breeding programs and for functional genomic approaches aimed at increasing knowledge of the biology of grapevine.

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Section: Resultscontrasting

confidence: 67%

Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

Prado

Rodriguez²,

Rey

et al. 2010

Plant Cell Tiss Organ Cult

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“…induced by in vitro culture, showing that most embryogenic calli maintained in medium containing 2,4-D for approximately 5 years maintained their ploidy level (Winkelmann et al 1998). In the same way, tetraploid plants of Quercus robur L. were identified in 8% of clones tested over 7 years in culture (Endemann et al 2001). Relative ploidy stability was observed for Quercus suber L. during the process of somatic embryogenesis (Loureiro et al 2005).…”

Section: Flow Cytometrymentioning

confidence: 65%

Ploidy stability of somatic embryogenesis-derived Passiflora cincinnata Mast. plants as assessed by flow cytometry

Pinto¹,

Barros²,

Viccini

et al. 2010

Plant Cell Tiss Organ Cult

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In this study, flow cytometric analysis was used to evaluate the genetic stability of Passiflora cincinnata Mast. plants regenerated via primary and secondary somatic embryogenesis. Embryogenic calli obtained from culturing zygotic embryos on Murashige and Skoog (MS) medium containing 18.1 lM 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.4 lM benzyladenine (BA) were transferred to differentiation medium. Torpedo and cotyledonary embryos were obtained. These primary embryos were maintained on differentiation medium to generate secondary embryos. Conversion of primary and secondary embryos yielded 305 and 138 normal plants, respectively. Almost 90% of plantlets survived following acclimatization. Flow cytometric analysis revealed that seed-derived plants had on average 3.01 pg nuclear DNA (2C), and all plants, except for a single plant regenerated via primary embryogenesis, maintained their ploidy. This single plant contained more than twice the average DNA content: 6.21 pg (4C). Epidermal stomata of leaves of the tetraploid plant were larger but lower in density than those of diploid plants, indicating that stomatal characteristics are useful in distinguishing between diploid and tetraploid plants of passion fruit. In summary, the procedure we employed to regenerated P. cincinnata plants via somatic embryogenesis generated mostly genetically true-to-type plants.

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“…In contrast, the occurrence of somaclonal variation in somatic embryogenesis, detected by SSRs and other molecular markers has been reported by several authors for other woody species such as Prunus persica (Hashmi et al 1997), Carya illinoinensis (Vendrame et al 1999), Quercus spp. (Hornero et al 2001;Endemann et al 2001;Wilhelm et al 2005;Lopes et al 2006) and Pinus sylvestris (Burg et al 2007).…”

Section: Resultsmentioning

confidence: 99%

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Lopes

Capelo

Brito

et al. 2008

Trees

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The crop species Olea europaea L. (olive tree) is of great economic importance in the Mediterranean region. Hence, many efforts have been done in the last decades to propagate this commercially valuable species by in vitro methods. On the other hand, the lesser known Olea maderensis (Lowe) Rivas Mart. & Del Arco which is a native species of the Madeira Archipelago has only been the subject of micropropagation from nodal stem cuttings. Therefore, in this work we analysed the stability of ten nuclear simple sequence repeat (SSR) markers at successive stages of the somatic embryogenesis process in two adult trees belonging to these two species from the Madeira Archipelago. For the induction of somatic embryogenesis, petiole and leaf explants were cultivated on solid Murashige and Skoog medium (MS) with 12.25 lM indole-3-butyric acid (IBA) and 4.56 lM of zeatin, in the dark. After 3 months, different callus tissues (non-embryogenic, pre-embryogenic and embryogenic) developed from leaf explants and petioles were later transferred to MS medium without growth regulators in the dark. All ten SSR markers were able to distinguish between species. However, no mutations were found at the SSR loci at any of the successive developmental stages from PEMs (pre-embryogenic masses) to somatic embryos. This genetic uniformity was observed within material derived from each genotype/species and its respective donor plant. Therefore, we conclude that the genomic integrities of both O. europaea and O. maderensis were maintained throughout the stages of the embryogenic processes in study suggesting the absence of somaclonal variation.

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Assessment of Age-Related Polyploidy in Quercus Robur L. Somatic Embryos and Regenerated Plants Using DNA Flow Cytometry

Cited by 52 publications

References 25 publications

Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

Ploidy stability of somatic embryogenesis-derived Passiflora cincinnata Mast. plants as assessed by flow cytometry

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

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