Assessment of anti-nucleosome antibody (ANuA) isotypes for the diagnosis and prediction of systemic lupus erythematosus and lupus nephritis activity

Zeng, Yanli; Xiao, Yun; Zeng, Fan-Xiang; Li, Jiang; Yan, Shuidi; Wang, Xuelian; Lin, Qiaoduan; Yu, Liang; Lu, Xinxin; Zhang, Yan; Yuan, Lin

doi:10.1007/s10238-022-00942-w

Cited by 5 publications

(1 citation statement)

References 30 publications

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“…To demonstrate that these IgA autoantibodies were also present in our patient's blood, we measured their levels with the same approach in serum samples. In this regard, we found that circulating IgA anti-dsDNA or IgA antinucleosome levels were highly increased in SLE patients, as expected, since they have been reported as elevated previously (50,51).…”

Section: Discussionsupporting

confidence: 88%

Salivary IgA subtypes as novel disease biomarkers in systemic lupus erythematosus

Romero‐Ramírez

Sosa-Hernández²,

Cervantes‐Díaz³

et al. 2023

Front. Immunol.

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IntroductionImmunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially expressed in mucosal sites like the colon. In clinical practice, immunoglobulins are typically measured in venous or capillary blood; however, alternative samples, including saliva, are now being considered, given their non-invasive and easy collection nature. Several autoimmune diseases have been related to diverse abnormalities in oral mucosal immunity, such as rheumatoid arthritis, Sjogren’s syndrome, and systemic lupus erythematosus (SLE).MethodsWe decided to evaluate the levels of both IgA subtypes in the saliva of SLE patients. A light chain capture-based ELISA measured specific IgA1 and IgA2 levels in a cohort of SLE patients compared with age and gender-matched healthy volunteers.ResultsSurprisingly, our results indicated that in the saliva of SLE patients, total IgA and IgA1 subtype were significantly elevated; we also found that salivary IgA levels, particularly IgA2, positively correlate with anti-dsDNA IgG antibody titers. Strikingly, we also detected the presence of salivary anti-nucleosome IgA antibodies in SLE patients, a feature not previously reported elsewhere.ConclusionsAccording to our results and upon necessary validation, IgA characterization in saliva could represent a potentially helpful tool in the clinical care of SLE patients with the advantage of being a more straightforward, faster, and safer method than manipulating blood samples.

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Section: Discussionsupporting

confidence: 88%