Assessment of anti-oxidant, anti-epileptic and anti-anxiety activities of pomegranate leaf (Punica grantum) in mice

Abstract

The aim of this research was to assess the anti-oxidant, anti-epileptic and anti-anxiety properties of the flavonoids obtained from the Pomegranate ( L.). Methanol and ethyl Punica granatumMaterials and Methods: acetate solvent was used for Soxhlet extraction and water was used for cold maceration and the extracts were screened for the presence of different phytochemicals. Anti-oxidant activity was assessed using DPPH method by using ascorbic acid as standard and IC50 value was calculated.Flavonoid obtained from the methanolic Results and Conclusion: extract showed highest free radical scavenging activity with IC50 value of 31.48µg/ml. Anti-epileptic activity was assessed using the MES model using Phenytoin (25 mg/kg) as standard and MEP and EAP were given at the doses of 100mg/kg and 200 mg/kg in which 200mg/kg methanolic extract showed the least duration of tonic clonic convulsion (26.67 0.33 sec). Actophotometer test was done to evaluate the anti-anxiety activity in which Diazepam (3 mg/kg) + standard drug was used and MEP and EAF (100mg/kg and 200mg/kg) was administered to the mice. The result showed that the MEF 200mg/kg showed the least movement during the 3 minutes time.