Assessment of antibacterial activity of crude leaf and root extracts of <i>Cassia alata</i> against <i>Neisseria gonorrhea</i>.

Abstract

Background: Gonorrhea is a major sexually transmitted disease worldwide and for its control, effective treatment is essential. However as more strains of Neisseria gonorrhoeae continuously develop resistance to several drugs, this strategy obliges scientists to discover newer effective drugs. Objectives: To ascertain whether crude leaf and root extracts of Cassia alata (Caesalpiniaceae) have antimicrobial activity against clinically resistant Neisseria gonorrhoeae bacteria. To determine and compare the MICs of their ether and methanol extracts. Materials and methods: Ether and methanol extracts were prepared from the plant parts. 12-375mg/ml of serially diluted ether extracts in DMSO and methanol extracts in water were tested using agar-well diffusion method against Neisseria gonorrhea clinical isolate cultured on MTM agar. MICs were determined from corresponding concentration-response curves. Ceftriaxone was used as positive control, whereas DMSO and water as negative controls. Results: All the crude extracts showed concentration-dependent Neisseria gonorrhea inhibition. Ether extracts for both leaves and roots gave lower MICs compared to those of methanol. Ether root extract showed the highest potency. Conclusions: Both the leaf and the root of Cassia alata plant have activity against clinically resistant Neisseria gonorrhoeae; the root having the higher activity. Lipophilic solvent, ether, give more potent antigonorrhoeal extracts. As expected Cassia alata plant in Central Uganda also has antibacterial activity.

Activity of the Medicinal Plant Parkia Javanica Against Neisseria Gonorrhoeae Multi Drug Resistant and Other Clinical Isolates

“…These MIC values were much higher than those reported in similar studies [25,26]. The MIC concentrations observed in our study were, however, lower than those reported by Kuete et al [27].…”

Antifungal Activity and Fourier Transform Infrared Spectrometric Characterization of Aqueous Extracts of Acacia senegal and Acacia tortilis on Phytopathogenic Fungi

“…Although several studies have evaluated the antimicrobial activities of gum exudates against a broad range of pathogens [7, [26][27][28][29], antifungal activity against the plant pathogenic fungi viz., A. alternata, H. rostratum, and F. solani remains highly elusive. Therefore, the present study explored the in vitro antifungal activity of aqueous extracts of A. senegal and A. tortilis against three plant pathogenic fungi (A. alternata, H. rostratum and F. solani).…”

Senna alata leaf extract restores insulin sensitivity in high-fat diet-induced obese mice

“…It has numerous pharmacological activities such as antitumor [10], anthelmintic [11], anti-bacterial [12], anti-oxidant and antiinflammatory [13], and anti-diabetic activities [14]. A recent study found that S. alata (250 and 500 mg/kg) improved abnormal lipid metabolism by suppressing hepatic lipogenic genes, sterol regulatory element binding protein 1c (SREBP1c), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) [15].…”