Assessment of anticancer activity of <i>Pulicaria undulata</i> on hepatocellular carcinoma HepG2 cell line

Emam, Manal A.; Khattab, Hemmat; Hegazy, Marwa G. A.

doi:10.1177/1010428319880080

Cited by 30 publications

(23 citation statements)

References 47 publications

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“…The total phenolics and flavonoids contents in the P. undulata were quantitatively determined [2,28]. Phenolics and flavonoids contents were measured as gallic acid, and Mass fragmentation patterns for other compounds in Table 1 are available in the Supplementary File.…”

Section: Total Phenolics and Flavonoids Contentsmentioning

confidence: 99%

Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert

Mohammed

Al-Omar

Khan

et al. 2021

Plants

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Pulicaria undulata (L.) C. A. Mey has multiple uses as part of traditional medicament, and several biological activities of the plant have been corroborated in the scientific literature. The current work evaluates the phytochemical constituents and biological properties of the water-ethanol extract of the P. undulata growing in Qassim, the central arid regions of Saudi Arabia. Qualitative UPLC-ESIQ-TOF analysis identified 27 compounds belonging to the phenolics, flavonoids, triterpenes, coumarins, and fatty acids chemical class. The quantitative analysis exhibited 33.3 mg/g GAE (Gallic Acid Equivalents), and 10.8 mg/g QE (Quercetin Equivalent) of the phenolics and flavonoids in the plant’s concentrated (to dryness) water-ethanol extract. The trace elements analysis of the plant’s dry powder established the presence of copper (20.13 µg/kg), and zinc (68.2 µg/kg) in the higher levels of occurrences. In terms of the antioxidant potential of the plant’s extract, the ferric-reducing, and free-radicals scavenging activities were recorded at 47.11 mg/g, and 19.13 mg/g equivalents of the concentrated to dryness water-ethanol extract of the plant. The water-ethanol extract of P. undulata also exhibited antimicrobial activity against the tested Gram-positive bacteria, while no activity was observed against the tested Gram-negative bacteria, or the fungi. The MIC (minimum inhibitory concentration) values were in the range of 49 to 1563 µg/mL, whereas the MBC (minimum bactericidal concentration) values ranged from 49 to 3125 µg/mL, against the tested Gram-positive bacteria. The P. undulata water-ethanol extract also exhibited potent cytotoxic effects with the IC50 value at 519.2 µg/mL against the MCF-7 breast cancer cell-lines, followed by the anticancer activity of erythroleukemic cell-lines, K562 at 1212 µg/mL, and pancreatic cell-lines, PANC-1, at 1535 µg/mL, as compared to the normal fibroblast cells (4048 µg/mL). The Annexin-V assay demonstrated that, as the P. undulata extract’s dose increased from IC50 to twice of the IC50, the percentage of necrosis was found to be increased in the late apoptosis of the cancer cells. These data confirmed the P. undulata extract’s ability to inhibit several human cancer cell lines’ growth in comparison to other local halophytes. The antimicrobial activity of the plant was also confirmed.

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Section: Total Phenolics and Flavonoids Contentsmentioning

confidence: 99%

Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert

Mohammed

Al-Omar

Khan

et al. 2021

Plants

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“…While the crude methanol extract recorded the lowest cytotoxic effect against HEPG2, MCF7 and HCT 116 with (IC 50 = 51.4, 105.1 and 86.7 µg / ml, respectively). AnEgyptian study by (Emam et al, 2019) recorded that methanol crude extract of P. undulata has cytotoxic activity against HEPG2 with IC 50 = 27.7 mg /ml and Hussien et al (2016) reported that the crude extract (CH 2 Cl 2 /MeOH) of P. undulata showed excellent cytotoxic activity against both MCF-7 cells and HEPG2 cells with IC 50 41.6 and 40.7 µg/ml respectively.…”

Section: Cytotoxic Activitymentioning

confidence: 99%

“…The genus Pulicaria that is belonging to Asteraceae includes about 75 species distributed widely in Asia, Africa, and Europe (Kalwij, 2012). They used in traditional medication as antihyperglycemic, and antispasmodic drugs, also they show anticancer, antioxidant and antimicrobial properties (Emam et al, 2019). The chemical elaboration of Pulicaria species revealed the presence of various SMs, such as mono-, sesqui-, and diterpenoids, lavonoids, and phenolics (Hegazy and Emam, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antitumor, Antimicrobial activities and Phytochemicals Constituent of different Extracts of Pulicaria undulata (Forssk.) Oliver. Grown Naturally in Saudi Arabia

Alshehr

Ghobashy

2020

ijrps

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Antitumor and antimicrobial resistance are a habitual global issue, which continually demands finding new natural compounds to encounter the resistance. Pulicaria undulata (Forssk.) Oliver. (Asteraceae family) has numerous promising medicinal properties. The recent work aimed at determination of antitumor effects of three extracts of P. undulata on three types of human carcinoma; HEPG-2 hepatocellular carcinoma, MCF-7 breast carcinoma and HCT-116 colon carcinoma cell lines. Anticancer activity was assessed through studying the viability of the cancer cells and apoptotic pathway. Also, antimicrobial potency of different extracts was assessed against studied human pathogens (five Gram negative bacteria, two Gram positive bacteria and yeast). The results reveal that chloroform extract has different levels of cytotoxicity toward the three types of cancer cell lines. A considerable decline in cancer cell rates has been linked to increasing in concentration of plant extract. The half maximal inhibitory concentration IC 50 value was 3.01 µg/ mL for the HepG-2, 16.4 µg/mL for the MCF-7, and 7.4 µg/ mL for HCT-116. Followed by the ethyl acetate extract which showed strong cytotoxic activity against HEPG2 with IC 50 = 12.2 µg ∕ml and moderate activities against MCF7 and HCT 116 and recorded (IC 50 = 26.7 and 26.4 µg ∕ml, respectively). While the crude methanol extract recorded the lowest cytotoxic effect against HEPG2, MCF7 and HCT 116 with (IC 50 = 51.4, 105.1 and 86.7 µg ∕ ml, respectively). Chloroform and ethyl acetate extracts have a high antimicrobial activity more than methanol extract against the pathogens being studied. HPLC and GCMs Analysis identified numerous chemical compounds of P. undulata extracts with various therapeutic benefits. In conclusion, P. undulata has the potential to act as an antimicrobial agent against various pathogenic microbes and is a promising wild herb for the treatment of cancer.

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“…Significant biological activities have been reported for Pulicaria sp. such as antispasmodic [ 13 ], antioxidant and cytotoxic activity [ 14 ], antimicrobial activity [ 15 ] and antihistaminic activity [ 16 ]. Previous phytochemical investigation on Pulicaria sp.…”

Section: Introductionmentioning

confidence: 99%

VEGFR-Mediated Cytotoxic Activity of Pulicaria undulata Isolated Metabolites: A Biological Evaluation and In Silico Study

Elhady

Abdelhameed

Zekry

et al. 2021

Life

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Natural products play a remarkable role not only in the synthesis, design, and discovery of new drugs but also as the most prominent source of drugs and bioactive substances. Adding to the search for new sources of safe innovative antitumor drugs, here we reported a phytochemical study on Pulicaria undulata which revealed promising antiangiogenic agents. Six compounds were isolated and identified as xanthoxyline (1), stigmasterol (2), oleanolic acid (3), salvigenin (4), rhamnetin (5) and dihydroquercetin-4′-methyl ether (6) using nuclear magnetic resonance (NMR) spectroscopic techniques. Compound 3 and 4 are first reported in Pulicaria genus. Both the extract and isolated compounds were evaluated for in vitro antiproliferative activity against breast cancer cell line (MCF-7). In vivo antiproliferative activity against Ehrlich’s ascites carcinoma (EAC) were also assessed. The P. undulata extract and isolates showed significant reduction in tumor weight, decreased both serum vascular endothelial growth factor B (VEGF-B) levels and vascular endothelial growth factor receptor 2 (VEGFR-2) expression significantly compared to the control EAC group, suggesting an antiangiogenic activity through the inhibition of VEGF signaling. Besides, they displayed reduction in CD34 expression, confirming their antiangiogenic effect. Moreover, the potential affinity of isolated compounds to human estrogen nuclear receptor-alpha (hER-α), the most recognized modulator of VEGFR-2 expression, was virtually estimated through molecular modeling studies. The most promising activity profiles were assigned to the investigated flavonoids, compounds 4–6, as well as the alkyl-phenylketone, compound 1. Additionally, these four top active compounds showed respective high to intermediate docking scores while possessing preferential binding with hER-α critical pocket residues. Based on the provided data, these isolated compounds illustrated promising inhibitors of VEGF-stimulated angiogenesis, which could be a possible mechanism for their anticancer activity.

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Assessment of anticancer activity of Pulicaria undulata on hepatocellular carcinoma HepG2 cell line

Cited by 30 publications

References 47 publications

Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert

Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert

Antitumor, Antimicrobial activities and Phytochemicals Constituent of different Extracts of Pulicaria undulata (Forssk.) Oliver. Grown Naturally in Saudi Arabia

VEGFR-Mediated Cytotoxic Activity of Pulicaria undulata Isolated Metabolites: A Biological Evaluation and In Silico Study

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