Assessment of antioxidant activity in Vaucheria sessilis extracts and their efficacy against isolated Candida spp. from diabetic foot ulcers

Abstract

This study was carried out in order to assess the biological efficacy of separated extracts of V. sessilis as an Antioxidant and antifungal against isolated candida spp. from diabetic foot ulcers. Several tests were used to identify the presence of active chemical compounds in V. sessilis and we note that the V. sessilis is rich in active substances. showed phytochemical results were found for the extracted ethanol 70% contain carbohydrates, flavonoids, phenols, alkaloids, glycosides, triterpenoid and saponin, and negative detection for Tannin, Steroid, and Amino acid. The antioxidant activity of V. sessilis extracts was assessed by decreasing the radical scavenging activity of (1,1-diphenyl-2-picrylhydrazyl (DPPH). Results exhibited that all extracts have antioxidant activity with different concentrations, showed the superiority of the polyphenol 86.02 % inhibition ratio at (1000 μg/ ml), flavonoid 84.05 % at (1000 μg/), and hexane extract 68.5 % at (1000 μg / ml) compared to ascorbic acid, which recorded 81.17% at a concentration of 1000 μg/ml. Whereas, the hexane extract recorded the lowest inhibition rate at (50 μg /ml). Efficacy showed of separated extracts from V. sessilis as antifungal activity using well diffusion plate method. The polyphenol extract (VP) was the highest activity against all isolated candida spp. from foot ulcers of diabetic patients, while the other extracts showed varying activity against different candida spp.at P. value < 0.01. The results that a high Zone rate of inhibition/mm 2 was (26.7 ± 0.6) against C. albicanis, while no inhibition/mm 2 (9.8± 0.8) for hexane extract against the same candida. HPLC analysis indicated the presence of 7 compounds in separated polyphenol extract of V.sessilis (Chlorogenic acid, Ferulic acid, Gallic acid, Rutin, Qurcetine, Kaempferol and Apigenin) with specific retention times. The detected compounds possess antioxidant and antimicrobial activities. These results suggested that separated extracts from V. sessilis have high antioxidant and antifungal activities. In the future, V. sessilis extracts could become one of the important alternatives in infection treatment, even if in a limited way due to the spread of candida spp. that are resistant to antifungals.