Assessment of Antioxidant and Anticancer Activities of Microgreen Alga Chlorella vulgaris and Its Blend with Different Vitamins

Abstract

There is a very vital antioxidant extracted from microgreen alga. Chlorella vulgaris has major advantages and requires high yield worldwide. Some microalgae require vitamins for their growth promotion. This study was held to determine the impact of different vitamins including Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), and Ascorbic acid (c) at concentrations of 0.02, 0.04, 0.06, and 0.08 mg/L of each. Each vitamin was added to the BG11 growth medium to determine the effect on growth, total carbohydrate, total protein, pigments content, antioxidant activities of Chlorella vulgaris. Moreover, antitumor effects of methanol extract of C. vulgaris without and with the supplement of thiamine against Human prostate cancer (PC-3), Hepatocellular carcinoma (HEPG-2), Colorectal carcinoma (HCT-116) and Epitheliod Carcinoma (Hela) was estimated in vitro. C. vulgaris supplemented with various vitamins showed a significant increase in biomass, pigment content, total protein, and total carbohydrates in comparison to the control. Thiamine was the best vitamin influencing as an antioxidant. C. vulgaris supplemented with thiamine had high antitumor effects in vitro. So, it’s necessary to add vitamins to BG11 media for enhancement of the growth and metabolites.

Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds

“…The AE of Chlorella sorokiniana also showed high cytotoxicity (IC 50 = 59.21 ± 10.3 µg/mL) against the drug-resistant HCT-15 CRC line, although in this case, cytotoxic activity decreased (IC 50 = 165.41 ± 7.34 µg/mL) when the AE was obtained after a treatment with proteases (HP), indicating the possible contribution of a protein in the antiproliferative activity of this drug-resistant cell line. Our results are even more promising than those reported in the literature obtained with various extraction methods from different species of Chlorella [21,22,37]. Regarding the two archaea species analyzed, it should be noted that of the different extraction methods used, only the AE and HP showed antiproliferative activity against CRC cells.…”

“…A methanolic extract of this microalga was tested on hepatocellular carcinoma (HEPG-2), human prostate cancer (PC-3) and colorectal cancer (HCT-116) cell lines. The data obtained show that for the colorectal cancer line there was an inhibition of cell growth of around 70% at a dose of 100 µg/mL [21]. The ethanolic extract of Chlorella vulgaris has also been studied in vitro in lung (A549), breast (MCF7), cervical (Hela), and hepatocellular (Huh7) cancer cell lines, showing an inhibition of cell growth in all lines tested [22].…”

The Influence of Diet and Its Components on the Development and Prevention of Hepatocellular Carcinoma (HCC)

“…Carotenoids and epigallocatechin gallate have been identified in studies using the applied extract [ 97 ]. Ragaa A. Hamouda et al demonstrated the anti-cancer effect of methanol extract from chlorella on HCC [ 98 ]. In animal studies with developed non-alcoholic fatty liver disease, alga supplementation improved the insulin sensitivity and lipid profile and reduced inflammation [ 99 ].…”

Complementary and Alternative Therapies in Oncology

“…Animal and in vitro studies have shown its antiproliferative effects on liver and colorectal cancer cells [6,7]. Lycopene isolated from chlorella inhibited the growth of prostate cancer cells in [64]. In an animal model, chlorella extract reduced bone marrow suppression caused by cisplatin [8].…”