Assessment of antioxidant and antidiabetic properties of Picralima nitida seed extracts

Abstract

The study investigated the antioxidant potential as well as the inhibitory potential of the seed extracts of Picralima nitida on α-amylase and α-glucosidase enzymes. The methanolic, aqueous and coconut water extracts were obtained using 70% methanol, distilled water and coconut water, respectively. Antioxidant properties were studied in vitro using DPPH (1, 1-diphenyl-2-pricrylhydrazyl) radical scavenging, Ferric Reducing Antioxidant Power (FRAP), Total Antioxidant Capacity (TAC), Hydroxyl Radical Averting Capacity (HORAC), Total Phenol Content (TPC), and Total Flavonoid Content (TFC) assays. Different concentrations (0.1 -0.5 mg/ml) of the extracts were subjected to α-amylase and αglucosidase inhibitory assays using acarbose as standard. Absorbance was measured at 540 (for αamylase) and 405 nm (α-glucosidase). The percentage of α-amylase and α-glucosidase inhibitory activity of the extracts and their IC 50 values were determined. The seed extracts of P. nitida showed significant antioxidant potential. The inhibitory activity of the extracts on α-amylase and α-glucosidas compared favourably with acarbose with the methanolic extract possessing the highest inhibitory activity. The methanolic extract also possessed the highest antioxidant capacity with the lowest IC 50 value among the extracts. The results indicate that the seed extracts of P. nitida possess significant antioxidant properties and are effective inhibitors of α-amylase and α-glucosidase enzymes.

Phytochemical Composition, Antioxidant Activity and Toxicity of Aqueous Extract of Picralima nitida in Drosophila melanogaster

“…Previous studies have shown that various extracts of the plant are good sources of these compounds. 6,7,40,41 The result obtained from the study also showed that APN could scavenge DPPH. The ascorbic acid, however, was able to scavenge DPPH more effectively than APN.…”

Chemical composition and in vitro antioxidant properties of water‐soluble extracts obtained from Bangladesh vegetables

“…The differences between our work and previous studies may be due to the type of extraction solvent used because some of the previous studies used solvents and not water. For example, the TPC of picralima seed from aqueous extraction was reported to be 435.87 ± 1.76 mg GAE/g, whereas it was 356.53 ± 4.67 mg GAE/g for the methanol extract (Akinwunmi & Amadi, 2019). Similarly, the TPC content of date plum water extract was 14.5 ± 0.20 mg GAE/g when compared to 1.6 ± 0.12 mg GAE/g for the acetone extract (Gao et al., 2014).…”

Ethnobotanical survey and phytogeographical study of plants species from genus Acacia in Bnin

“…Leaves were the most used part. Many previous studies showed the use of leaves in the preparation of various recipes in traditional medicine (Akinwunmi and Amadi, 2019;Zahoor et al, 2017;Odewo and Adeyemo, 2018;Balcha, 2014).…”