Assessment of antioxidant and xanthine oxidase inhibitory activity of Triadica cochinchinensis stem bark

Abstract

Triadica cochinchinensis has been used traditionally to treat diseases related to oxidative stress. Nonetheless, little is known about its biological activity. This study aimed to evaluate the antioxidant and xanthine oxidase inhibitory activity of this plant. For this purpose, qualitative phytochemicals, total phenolic, total flavonoid, antioxidant assays (DPPH and FRAP), and xanthine oxidase assay were evaluated towards methanol and organic fractions of its stem bark. Results showed the occurrence of flavonoids, tannins, terpenoids, steroids, and saponins in the methanol extract. The extract and its two fractions (ethyl acetate and methanol) exhibited promising radical scavenging (IC50 values between 3.6-4.5 μg/mL) and ferric reduction activities (4.2-5.5 μg/μg of equivalent trolox amount). They also exhibited inhibition on the xanthine oxidase activity ranging from 43.8 to 80.5% at a dose of 100 μg/mL. These activities could be attributed to the amount of phenolics in the methanol extract and active fractions (136.6-174.1 mg GAE/g of sample). Our results suggested that the methanol extract of T. cochinchinensis stem bark could be used as a promising source of lead molecules for antioxidant and xanthine oxidase inhibitors from natural source.

Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits

“…According to our previous study with modifications 5,7 , total phenolics in the ethanol extract and organic fractions of M. scabra fruits were evaluated spectrophotometrically using the Folin-Ciocalteu (FC) method. A linear gallic acid standard curve was first established within a concentration range of 6.25 to 100 µg/mL versus absorbance of molybdenum-tungsten blue complex at 635 nm.…”

“…Total flavonoids content in the ethanol extract and organic fractions of M. scabra fruits was evaluated spectrophotometrically using the aluminum chloride method as previously reported with modifications 5,7 . A linear quercetin standard curve was first established within a concentration range of 1.56 to 100 µg/mL versus the absorbance of the yellow complex at 425 nm.…”

“…DPPH assay was carried out to determine the radical scavenging activity of ethanol extract and organic fractions of M. scabra fruits towards free radicals 5,7 . The assay was performed qualitatively using dot-blot staining on TLC plate and quantitatively using the spectrophotometric method.…”

(−)-Gallocatechin Gallate: A Novel Chemical Marker to Distinguish Triadica cochinchinensis Honey

“…Triadica cochinchinensis is a tree or shrub belonging to the Euphorbiaceae family ( Figure 1 A) and one of the main nectar plants in southern China during summer time, showing a long flowering stage and high nectar production [ 16 , 17 ]. Traditionally used in Chinese herbal medicine, its leaves, bark and roots can be used to treat various internal pathological conditions, including nephritis, oedema, ascites, constipation and dysuria, as well as external pathologies, such as allergic dermatitis, mastitis, bruises, wounds and snakebites [ 18 , 19 ]. Recent studies have shown that T. cochinchinensis leaf and stem parts contain various bioactive compounds, including diterpenoids, phenolics, flavonoids and tannins, which are the main components imparting antioxidant, anti-inflammatory, antibacterial, hepatoprotective and antidiabetic effects [ 20 , 21 ].…”