Assessment of antioxidant, antidiabetic, antiobesity, and anti-inflammatory properties of a Tannat winemaking by-product

Fernández-Fernández, Adriana Maite; Iriondo‐DeHond, Amaia; Dellacassa, Eduardo; Medrano-Fernández, Alejandra; Castillo, M. Dolores del

doi:10.1007/s00217-019-03252-w

Cited by 37 publications

(61 citation statements)

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“…Seeds and skins of Tannat grape pomace were manually separated and the skin was submitted to drying at 40 • C up to constant weight, achieved at 24 h [3]. Skin was then powdered by employing a domestic mill.…”

Section: Grape Pomace Treatment and Extract Preparationmentioning

confidence: 99%

“…During red winemaking, anthocyanins still remain in high amount at the pomace due to skin matrix retention [2]. Phenolic compounds of Tannat grape skin have been associated to antioxidant, antidiabetic, antiobesity, and anti-inflammatory properties [3].…”

Section: Introductionmentioning

confidence: 99%

“…TPC was performed by the Folin-Ciocalteau method [9] with modifications described by Fernández-Fernández et al [3] using a gallic acid standard curve ranged from 0.05 to 1.0 mg/mL. Results were expressed as mg GAE/g of dry sample.…”

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confidence: 99%

“…ABTS method [10] with modifications [3] was performed to determine electron transfer (ET) capacity using a trolox calibration curve (0.25 to 1.5 mM). Hydrogen atom transfer (HAT) capacity was performed by ORAC-FL assay [11] with some modifications [3]. Trolox calibration curve was constructed through the area under the curve (AUC) and results were expressed as µmol TE/g of dry sample.…”

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confidence: 99%

“…Previous to ROS determinations, cell viability was tested on CCD-18Co and RAW264.7 cells by MTT assay according to Fernández-Fernández et al [3] description to determine the possible cytotoxic effect of samples (extract and digest). Intracellular ROS were measured using the fluorescent probe DCFH-DA.…”

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confidence: 99%

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In Vitro Bioaccessibility of Extractable Compounds from Tannat Grape Skin Possessing Health Promoting Properties with Potential to Reduce the Risk of Diabetes

Fernández-Fernández

Iriondo‐DeHond

Nardin

et al. 2020

Foods

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Diabetes pathogenesis encompasses oxidative stress, inflammation, insulin malfunctioning and partial or total insulin secretion impairment, which leads to a constant hyperglycemia. Polyphenols are known to possess bioactive properties, being Tannat grape skin a natural and sustainable source of these compounds. The present study aimed to find out the bioaccessibility of health-promoting molecules composing a multifunctional extract from Tannat grape skin obtained under hydro-alcoholic-acid conditions. The identification of phenolic compounds in the samples was performed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Subsequently, the samples were in vitro digested mimicking the human oral gastrointestinal conditions and the bioactivity of the digest (antioxidant, anti-inflammatory and modulation of glucose metabolism) was assessed. Effect on glucose metabolism was estimated by measuring carbohydrases activity and the functionality of glucose transporters of small intestine cells in presence and absence of the digested extract. Flavonoids, phenolic acids and phenolic alcohols were the major phenol compounds detected in the extract. The bioaccessible compounds protected the intestinal cells and macrophages against the induced formation of reactive oxygen species (ROS) and nitric oxide (NO). In addition, glucose transporters were inhibited by the digested extract. In conclusion, the bioaccessible compounds of the extract, including phenols, modulated key biochemical events involved in the pathogenesis of diabetes such as oxidative stress, inflammation and glucose absorption. The extract was effective under prevention with co-administration conditions supporting its potential for either reducing the risk or treating this disease.

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Section: Grape Pomace Treatment and Extract Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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