Assessment of Apical Expression of Alpha-2 Integrin, Heat Shock Protein, and Proinflammatory and Immunoregulatory Cytokines in Response to Endodontic Infection

Bambirra, Wilson; Maciel, Kamilla Faria; Thebit, Marcela Marçal; Brito, Luciana Carla Neves de; Vieira, Leda Quércia; Sobrinho, Antônio Paulino Ribeiro

doi:10.1016/j.joen.2015.03.002

Cited by 27 publications

(26 citation statements)

References 36 publications

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“…() Orbis –2 mm past WL301After Instrumenting–ELISAPBS (300 µL)3003010 at − g Bambirra et al . ()––2 mm past WL602After Instrumenting7 daysqPCRTRIzol (−µL)10–25 at 12 000 g de Brito et al . ()––2 mm past WL602After Instrumenting7 daysqPCRTRIzol (−µL)10–25 at 12 000 g Ferreira et al .…”

Section: Resultsmentioning

confidence: 99%

“…Four studies used 1 point per sample(Wahlgren et al 2002, Liu et al 2003, Pezelj-Ribari c et al 2007, Shahriari et al 2011 whereas five used 2(Atao glu et al 2002, Alptekin et al 2005a,b, Ehsani et al 2012, Grga et al 2013, eleven used 3(Henriques et al 2011, Tavares et al 2012, Rechenberg et al 2014, Bambirra et al 2015, Martinho et al 2015, Sette-Dias et al 2016, four used ≤ 5…”

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confidence: 99%

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A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

et al. 2019

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Aim The primary aim was to identify techniques used to sample and analyse periradicular tissue fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach. Methodology Data Sources: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey. Eligibility Criteria: Clinical studies published until 1 June 2018 which utilized orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded. Study appraisal and methods: In accordance with PRISMA guidelines, data were extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted, and studies were critically appraised using a modified version of the Cochrane risk of bias tool. Results Study Characteristics: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n = 27), fine needle aspiration (n = 4) and filter strips (n = 2). Assay techniques included enzyme‐linked immunosorbent assay (n = 18), quantitative polymerase chain reaction (n = 9), radioimmunoassay (n = 4), colorimetric assay (n = 2), immunofluorometric assay (n = 1) and cytometric bead array (n = 1). Forty‐five different inflammatory mediators were targeted at the proteomic/metabolomic (n = 25) or transcriptomic level (n = 9). Limitations: Significant heterogeneity exists within the methodology, and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols. Conclusions Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during root canal treatment. The most studied analytes were IL‐1β and TNF‐α. Implications: Further research is required to develop an optimized PTF sampling and eluting protocol to overcome methodological heterogeneity, and future studies are advised to follow a standardized approach to reporting their methodology.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

et al. 2019

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“…Bambirra et al reported that MRNA expression of IL-8 and other pro-inflammatory cytokines decreased 7 days after chemo-mechanical debridement, due to the reduction in microbial load (14). Bambirra et al reported that MRNA expression of IL-8 and other pro-inflammatory cytokines decreased 7 days after chemo-mechanical debridement, due to the reduction in microbial load (14).…”

Section: Discussionmentioning

confidence: 99%

“…The pulp chamber was dried, and samples were collected by three paper points introduced consecutively into the canal to extend 2 mm beyond the apex (as measured radiographically) and left undisturbed for one minute (14). Access cavity was prepared using size #2 and #4 round tungsten carbide burs (Dentsply Sirona Endodontics, York, PA, USA).…”

Section: Sample Collectionmentioning

confidence: 99%

Effect of root canal debridement on inflammatory cytokine levels

et al. 2018

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In endodontic infections, inflammatory mediators such as cytokines are released, recruited and retained until the infection is eradicated. Root canal therapy is performed to prevent the spread of infection. The aim of this study was to investigate the effects of root canal debridement (cleaning and shaping) on periapical inflammation by measuring the levels of inflammatory cytokines, Interleukin-8 (IL-8) and Interleukin-10 (IL-10). The study includes twenty patients with pulp necrosis and asymptomatic apical periodontitis. Periradicular sample was collected using paper points before and after root canal debridement. Cytokine levels were determined by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). Data were analysed using paired t-test (PASW Statistics 18) (P = 0.05). All samples showed the presence of IL-8 and IL-10 prior to root canal debridement. Significantly reduced levels (P < 0.05) of IL-8 and IL-10 were detected after root canal debridement. In conclusion, root canal debridement significantly decreased the levels of the tested pro- and anti-inflammatory cytokine in the periradicular interstitial fluid.

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“…Although inflammation is an important biological process to protect the host and contain pathogens, excessive inflammation aggressively affects healthy tissues and may compromise tissue functionality (Bian et al 2012). In contrast, IL-10 is a cytokine that exhibits anti-inflammatory properties and plays a central role in reducing infection by limiting the immune response to pathogens and thus preventing damage to the host (Bambirra et al 2015). In contrast, IL-10 is a cytokine that exhibits anti-inflammatory properties and plays a central role in reducing infection by limiting the immune response to pathogens and thus preventing damage to the host (Bambirra et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Systemic administration of probiotics reduces the severity of apical periodontitis

Cosme‐Silva¹,

Dal‐Fabbro²,

Cintra³

et al. 2019

Int Endodontic J

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Aim To evaluate the effect of systemic administration of probiotics on the severity of apical periodontitis (AP). Methodology Twenty‐four male Wistar rats were used. AP was induced in the maxillary left/right first molars. The animals were arranged into groups: Control, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Probiotics were administered orally for gavage (109 colony‐forming units diluted in 5 mL of water for 30 days) during the development of AP. After 30 days, cardiac puncture was performed to analyse the complete blood count. Moreover, microbiological analysis of the root canal contents and saliva was performed. Then, the animals were euthanized and the jaw removed for histopathological and IL‐10, IL‐1β and IL‐6 immunolabeling analyses. After the Shapiro–Wilk test of normality, the Kruskal–Wallis followed by Dunn's test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P < 0.05). Results No significance difference was observed in the blood profiles and in the counts of microorganisms from the saliva samples among the groups (P > 0.05). Total microorganism counts in the root canal, the inflammatory infiltrate and the immunostaining for IL‐1β and IL‐6 in AP were significantly lower in the probiotic groups when compared with the control group (P < 0.05). IL‐10 was significantly more immunolabled in the probiotic groups than in the control group (P < 0.05). Conclusion Supplementation with probiotics (Lactobacillus rhamnosus and Lactobacillus acidophilus) had a significant effect on the severity of apical periodontitis in rats, demonstrating the anti‐inflammatory effect of probiotics on the development of apical periodontitis.

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Assessment of Apical Expression of Alpha-2 Integrin, Heat Shock Protein, and Proinflammatory and Immunoregulatory Cytokines in Response to Endodontic Infection

Cited by 27 publications

References 36 publications

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

Effect of root canal debridement on inflammatory cytokine levels

Systemic administration of probiotics reduces the severity of apical periodontitis

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