Assessment of autoimmunity-inducing potential using the Brown Norway rat challenge model

White, Kimber L.; David, Daniel W; Butterworth, Leon; Klykken, Paal C.

doi:10.1016/s0378-4274(99)00225-8

Cited by 17 publications

(12 citation statements)

References 11 publications

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“…This phenomenon is not unique and other inorganic salts, such as HgCl 2 , have been shown in the past to cause an elevation of total IgE in the BN rat [31]. As expected only the rats that also received HDM during sensitisation showed significant levels of serum HDM-specific IgE.…”

Section: Discussionsupporting

confidence: 72%

House dust mite induces direct airway inflammationin vivo: implications for future disease therapy?

Alba

Raemdonck²,

Dekkak³

et al. 2009

Eur Respir J

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House dust mite (HDM) is the major source of allergen in house dust and is strongly associated with the development of asthma. HDM can evoke a direct, nonallergic inflammatory reaction in vitro. We aimed to determine whether this apparent nonallergic, inflammatory response can be observed in a more complex in vivo setting.Vehicle, Alum TM or HDM (Dermatophagoides pteronyssinus 5 mg, i.p. with Alum) sensitisedBrown-Norway rats were challenged intratracheally with vehicle (saline), HDM (Der p 10 mg) or heat-inactivated HDM on day 21. Lung function changes and the associated inflammatory response were evaluated. Tissue and bronchoalveolar lavage from Alum TM sensitised Der p challenged animals exhibited strong eosinophilia and neutrophilia associated with an early release of pro-inflammatory cytokines (interleukin-13 and 1b, eotaxin and thymus and activation-regulated chemokine). This response was not attenuated by removal of HDM-associated protease activity. Interestingly, the vehicle sensitised group (no Alum TM ) lacked this inflammatory response.HDM allergen evokes nonallergic airways inflammation with an inflammatory profile similar to that of the asthmatic airway. This response, independent of the protease activity of the HDM extract, appeared to be linked to prior administration of the adjuvant Alum TM and the subsequent increase in total immunoglobulin E. This finding could have important implications in the development of future asthma therapies.

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Section: Discussionsupporting

confidence: 72%

House dust mite induces direct airway inflammationin vivo: implications for future disease therapy?

Alba

Raemdonck²,

Dekkak³

et al. 2009

Eur Respir J

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“…Similar findings were obtained in MRL +/+ mice following exposure to TCE for up to 22 weeks via the drinking water (Gilbert et al, 1999). In contrast to D-penicillamine and mercuric chloride, TCE produced no immune changes in BrownNorway rats, another autoimmunity-prone rodent strain (White et al, 2000).…”

Section: G Ravel Et Alsupporting

confidence: 76%

Trichloroethylene Does Not Accelerate Autoimmune Diabetes in NOD Mice

Ravel¹,

Christ²,

Perron-Lepage³

et al. 2005

Journal of Immunotoxicology

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Pre-existing or contributing risk factors, including genetic predisposition and environmental influences, are largely thought to play a crucial (though ill-elucidated) role in the development of autoimmunity. Trichloroethylene (TCE) is a widely used organic solvent, which has been suspected of increasing the prevalence of autoimmune diseases, e.g., lupus, following environmental contamination. Although few epidemiological data are available, several studies reported an accelerated and more severe disease in TCEexposed autoimmunity-prone MRL +/+ mice. To test whether TCE can exert similar deleterious effects on organ-specific autoimmune diseases, non obese diabetic (NOD) mice were given 5 mg/ml TCE via the drinking water for 12 weeks. TCE administration induced a decrease in CD44 + splenic T-cells and CD45RB high , CD54 + blood and splenic T-cells. Conversely, the number of CD45RB low splenocytes was increased. Interestingly, the progressive increase in serum TNF-α and IFN-γ levels normally seen with age in these mice was inhibited by TCE. There was also a relative lower incidence of histological changes in the pancreas of TCE-exposed NOD mice than in unexposed mice. Contrary to what has been found in systemic models of autoimmunity, TCE did not accelerate the diabetes of NOD mice and may have a protective effect. This finding suggests that comparative studies using different genetically related autoimmune-prone models are needed to investigate the role of xenobiotics in the precipitation of autoimmunity, particularly in sensitive populations.

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“…Also, a negative relationship was suggested between the TCE concentration and serum IgE (R 2 = -0.10). Inconsistent with our findings in exposed rats is the fact that a straight relationship between TCE inhalation and serum IgE levels has not been approved, with studies either reporting no effect or conflicting findings based on the concentration and length of the exposure period (17,18).…”

Section: Discussioncontrasting

confidence: 79%

Toxicological Effects of Inhalation Exposure to Trichloroethylene on Serum Immunoglobulin and Electrolyte Levels in Rats

et al. 2019

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Background: Trichloroethylene (TCE) is one of the most important environmental contaminants that can introduce both occupational and public health because of its widespread use. Objectives: This study was aimed to investigate effects of inhalation exposure to TCE on serum immunoglobulin and electrolyte levels under controlled laboratory conditions. Methods: Five equal groups (5 animals each) were randomly selected from a total of 25 adult male Sprague-Dawley rats. TCE vapor was generated by dynamic evaporation method in exposure chamber. The animals were exposed to TCE at five different concentration levels (0 ppm as control, 10, 100, 250, and 400 ppm through 8 hours inhalation during a day, for 10 consecutive days. Results: Based on results, IgG, IgM and IgA levels were significantly increased in rats following exposure to various levels of TCE ≥ 100 ppm (P < 0.05). Conversely, TCE exposure significantly caused a decrease in the levels of IgE in the case group with those values compared to control group (P < 0.05). Contrariwise, serum levels of Ca were significantly increased in rats exposed to TCE levels above 250 ppm, the high and very high exposure level (P < 0.05). At a concentration of 100 and 400 ppm, changes were noted in serum P levels (P < 0.05). A moderate positive relationship was found between serum P and the TCE concentration in sub-acute exposure (R 2 = 0.66, P < 0.05). Conclusions:In conclusion, sub-acute inhalation exposure to TCE (≥ 100 ppm) is related to the electrolyte and immunoglobulin toxicity in the form of increased Ca, P, IgA, IgG and IgM and decreased Cl, Na and IgE.

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Assessment of autoimmunity-inducing potential using the Brown Norway rat challenge model

Cited by 17 publications

References 11 publications

House dust mite induces direct airway inflammationin vivo: implications for future disease therapy?

House dust mite induces direct airway inflammationin vivo: implications for future disease therapy?

Trichloroethylene Does Not Accelerate Autoimmune Diabetes in NOD Mice

Toxicological Effects of Inhalation Exposure to Trichloroethylene on Serum Immunoglobulin and Electrolyte Levels in Rats

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